The quantitative analysis of constitutive heterochromatic regions of human chromosomes 1, 9, and 16 in relation to size and inversion heteromorphisms in East Indians

1982 ◽  
Vol 38 (3) ◽  
pp. 324-326 ◽  
Author(s):  
R. S. Verma ◽  
J. Rodriguez ◽  
H. Dosik
Keyword(s):  
Quantitative Analysis ◽  
Human Chromosomes ◽  
East Indians ◽  
And Inversion

The quantitative analysis of polymorphism on human chromosomes 1, 9, 16 and Y

1980 ◽  
Vol 54 (2) ◽  
pp. 163-169 ◽  
Author(s):  
O. A. Podugolnikova ◽  
A. P. Korostelev
Keyword(s):  
Quantitative Analysis ◽  
Human Chromosomes

Quantitative analysis of C-bands based on optical density profiles in human chromosomes

1977 ◽  
Vol 39 (2) ◽  
pp. 169-175 ◽  
Author(s):  
V. A. Benyush ◽  
V. G. Luckash ◽  
A. V. Shtannikov
Keyword(s):  
Quantitative Analysis ◽  
Optical Density ◽  
Human Chromosomes ◽  
Density Profiles

The quantitative analysis of polymorphism on human chromosomes 1, 9, 16, and Y

1979 ◽  
Vol 49 (3) ◽  
pp. 251-260 ◽  
Author(s):  
O. A. Podugolnikova ◽  
H. M. Sushanlo ◽  
I. V. Parfenova ◽  
A. A. Prokofieva-Belgovskaja
Keyword(s):  
Quantitative Analysis ◽  
Human Chromosomes

Analysis of centromere size in human chromosomes 1, 9, 15, and 16 by electron microscopy

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 710-713 ◽  
Author(s):  
Laura Sanchez ◽  
Paulino Martínez ◽  
Vicente Goyanes
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Chromosome 9 ◽  
Interindividual Variation ◽  
Pericentromeric Heterochromatin ◽  
Chromosome 15 ◽  
Chromosome Size ◽  
Human Chromosomes ◽  
Positive Correlation ◽  
Whole Mount

Human chromosomes were treated with 5-azacytidine and analyzed by whole-mount electron microscopy. This base analogue produces undercondensation of heterochromatin and separation of the centromere from the bulk of pericentromeric heterochromatin in chromosomes 1, 9, 15, and 16, which allows clear delimitation of the centromere regions. A quantitative analysis of centromeres showed that chromosomes 1, 9, and 16 have centromeres of different size. The centromere of chromosome 15 is similar in size to that of chromosome 9 and different from those of chromosomes 1 and 16. No interindividual variation for centromere size was found. A positive correlation between centromere and chromosome size was found for the chromosomes analyzed.Key words: centromere size, human chromosomes, electron microscopy.

The quantitative analysis of polymorphism on human chromosomes 1, 9, 16, and Y

1979 ◽  
Vol 49 (3) ◽  
pp. 243-250 ◽  
Author(s):  
O. A. Podugolnikova ◽  
I. V. Parfenova ◽  
H. M. Sushanlo ◽  
A. A. Prokofieva-Belgovskaja
Keyword(s):  
Quantitative Analysis ◽  
Human Chromosomes

An Improved Quantitative Image Analyser

1977 ◽  
Vol 35 ◽  
pp. 226-227
Author(s):  
J.P. Fallon ◽  
P.J. Gregory ◽  
C.J. Taylor
Keyword(s):  
Feature Extraction ◽  
Quantitative Analysis ◽  
Frequency Content ◽  
General Sense ◽  
Physical Measurements ◽  
Quantitative Image ◽  
Image Analyser ◽  
Automatic Methods ◽  
Quantitative Results

Quantitative image analysis systems have been used for several years in research and quality control applications in various fields including metallurgy and medicine. The technique has been applied as an extension of subjective microscopy to problems requiring quantitative results and which are amenable to automatic methods of interpretation.Feature extraction. In the most general sense, a feature can be defined as a portion of the image which differs in some consistent way from the background. A feature may be characterized by the density difference between itself and the background, by an edge gradient, or by the spatial frequency content (texture) within its boundaries. The task of feature extraction includes recognition of features and encoding of the associated information for quantitative analysis.Quantitative Analysis. Quantitative analysis is the determination of one or more physical measurements of each feature. These measurements may be straightforward ones such as area, length, or perimeter, or more complex stereological measurements such as convex perimeter or Feret's diameter.

The Fine Structure of the Sheath of Volvox Carteri f. Weismannia

1977 ◽  
Vol 35 ◽  
pp. 346-347
Author(s):  
Regina Birchem
Keyword(s):  
Developmental Stages ◽  
Ruthenium Red ◽  
Sulfated Polysaccharides ◽  
Volvox Carteri ◽  
High Voltage Electron Microscopy ◽  
Ultrastructural Studies ◽  
Voltage Electron ◽  
Sheath Formation ◽  
And Inversion ◽  
Sheath Material

Spheroids of the green colonial alga Volvox consist of biflagellate Chlamydomonad-like cells embedded in a transparent sheath. The sheath, important as a substance through which metabolic materials, light, and the sexual inducer must pass to and from the cells, has been shown to have an ordered structure (1,2). It is composed of both protein and carbohydrate (3); studies of V. rousseletii indicate an outside layer of sulfated polysaccharides (4).Ultrastructural studies of the sheath material in developmental stages of V. carteri f. weismannia were undertaken employing variations in the standard fixation procedure, ruthenium red, diaminobenzidine, and high voltage electron microscopy. Sheath formation begins after the completion of cell division and inversion of the daughter spheroids. Golgi, rough ER, and plasma membrane are actively involved in phases of sheath synthesis (Fig. 1). Six layers of ultrastructurally differentiated sheath material have been identified.

Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

1982 ◽  
Vol 40 ◽  
pp. 332-333
Author(s):  
V. V. Damiano ◽  
R. P. Daniele ◽  
H. T. Tucker ◽  
J. H. Dauber
Keyword(s):  
Quantitative Analysis ◽  
Alveolar Macrophages ◽  
Bulk Sample ◽  
Silica Particles ◽  
Empirical Method ◽  
Phagocytic Cells ◽  
Calibration Standards ◽  
X Ray ◽  
Accurate Quantitative Analysis ◽  
Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

Lateral resolution of the electron microbeam analysis

1978 ◽  
Vol 36 (1) ◽  
pp. 542-543
Author(s):  
H.J. Dudek
Keyword(s):  
Quantitative Analysis ◽  
Depth Resolution ◽  
Lateral Resolution ◽  
Cylindrical Particle ◽  
Correction Procedure ◽  
X Ray ◽  
Element Concentrations ◽  
Microbeam Analysis ◽  
The Matrix

The chemical inhomogenities in modern materials such as fibers, phases and inclusions, often have diameters in the region of one micrometer. Using electron microbeam analysis for the determination of the element concentrations one has to know the smallest possible diameter of such regions for a given accuracy of the quantitative analysis.In th is paper the correction procedure for the quantitative electron microbeam analysis is extended to a spacial problem to determine the smallest possible measurements of a cylindrical particle P of high D (depth resolution) and diameter L (lateral resolution) embeded in a matrix M and which has to be analysed quantitative with the accuracy q. The mathematical accounts lead to the following form of the characteristic x-ray intens ity of the element i of a particle P embeded in the matrix M in relation to the intensity of a standard S

Sign in / Sign up

Export Citation Format

Share Document