Analysis of centromere size in human chromosomes 1, 9, 15, and 16 by electron microscopy

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 710-713 ◽  
Author(s):  
Laura Sanchez ◽  
Paulino Martínez ◽  
Vicente Goyanes
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Chromosome 9 ◽  
Interindividual Variation ◽  
Pericentromeric Heterochromatin ◽  
Chromosome 15 ◽  
Chromosome Size ◽  
Human Chromosomes ◽  
Positive Correlation ◽  
Whole Mount

Human chromosomes were treated with 5-azacytidine and analyzed by whole-mount electron microscopy. This base analogue produces undercondensation of heterochromatin and separation of the centromere from the bulk of pericentromeric heterochromatin in chromosomes 1, 9, 15, and 16, which allows clear delimitation of the centromere regions. A quantitative analysis of centromeres showed that chromosomes 1, 9, and 16 have centromeres of different size. The centromere of chromosome 15 is similar in size to that of chromosome 9 and different from those of chromosomes 1 and 16. No interindividual variation for centromere size was found. A positive correlation between centromere and chromosome size was found for the chromosomes analyzed.Key words: centromere size, human chromosomes, electron microscopy.

Collecting Human Chromosomes for Whole-Mount Electron Microscopy

1974 ◽  
Vol 49 (3) ◽  
pp. 133-136
Author(s):  
C. Wolff ◽  
C. Gilly ◽  
C. Mouriquand
Keyword(s):  
Electron Microscopy ◽  
Human Chromosomes ◽  
Whole Mount

The application of video-enhanced constrast/differential interference constrast microscopy in conjunction with whole mount electron microscopy to the study of organelle transport

1988 ◽  
Vol 46 ◽  
pp. 66-67
Author(s):  
J. H. Hayden
Keyword(s):  
Electron Microscopy ◽  
Rana Pipiens ◽  
Silver Film ◽  
Immunofluorescence Microscopy ◽  
Organelle Transport ◽  
Linear Element ◽  
Whole Mount ◽  
Carbon Coated ◽  
Linear Elements ◽  
Dic Microscopy

In a previous study, Allen video-enhanced constrast/differential interference constrast (AVEC-DIC) microscopy was used in conjunction with immunofluorescence microscopy to demonstrate that organelles and vesicle move in either direction along linear elements composed of microtubules. However, this study was limited in that the number of microtubules making up a linear element could not be determined. To overcome this limitation, we have used AVEC-DIC microscopy in conjunction with whole mount electron microscopy.Keratocytes from Rana pipiens were grown on glass coverslips as described elsewhere. Gold London Finder grids were Formvar- and carbon coated, and sterilized by exposure to ultraviolet light. It is important to select a Formvar film that gives a grey reflection when it is floated on water. A silver film is too thick and will detract from the image in the light microscope.

Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 14-15
Author(s):  
Conly L. Rieder
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Light Microscopy ◽  
Living Cell ◽  
Light And Electron Microscopy ◽  
Time Behavior ◽  
Dynamic Interactions ◽  
Positive Identification ◽  
Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

The quantitative analysis of polymorphism on human chromosomes 1, 9, 16 and Y

1980 ◽  
Vol 54 (2) ◽  
pp. 163-169 ◽  
Author(s):  
O. A. Podugolnikova ◽  
A. P. Korostelev
Keyword(s):  
Quantitative Analysis ◽  
Human Chromosomes

scholarly journals Staining and Embedding of Human Chromosomes for 3-D Serial Block-Face Scanning Electron Microscopy

BioTechniques ◽  
2014 ◽  
Vol 57 (6) ◽  
Author(s):  
Mohammed Yusuf ◽  
Bo Chen ◽  
Teruo Hashimoto ◽  
Ana Katrina Estandarte ◽  
George Thompson ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Chromosomes ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron
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