Production and characterization of monoclonal antibodies against tissue specific epitopes on ABO blood group substances in saliva

1991 ◽  
Vol 104 (4) ◽  
pp. 193-196 ◽  
Author(s):  
Akihiko Kimura ◽  
Fuseo Matsumura ◽  
Ken-ichiro Sodesaki ◽  
Tsutomu Tsuji
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Group ◽  
Abo Blood Group ◽  
Tissue Specific ◽  
Blood Group Substances

A rheo-optical method for the study of aggregated particles: application to the characterization of ABO blood group monoclonal antibodies

2000 ◽  
Vol 39 (6) ◽  
pp. 595-600
Author(s):  
Jean-François Stoltz ◽  
Rodolphos Jose Rasia ◽  
Juana Rosa Valverde ◽  
L. Pla ◽  
Danielle Sontag ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Group ◽  
Optical Method ◽  
Abo Blood Group

Methods for detecting multiple blood-meals in mosquitoes (Diptera, Culicidae)

1976 ◽  
Vol 66 (4) ◽  
pp. 671-679 ◽  
Author(s):  
P. F. L. Boreham ◽  
J. K. Lenahan
Keyword(s):  
Aedes Aegypti ◽  
Blood Group ◽  
Anopheles Stephensi ◽  
Abo Blood Group ◽  
Multiple Blood ◽  
Blood Group Substances ◽  
Multiple Feeding ◽  
The Tropics ◽  
Short Time ◽  
Blood Meals

AbstractTwo techniques have been developed to investigate the incidence of multiple feeding by mosquitoes. One system detects the ABO blood group substances and can be used up to 24 h after feeding in the case of Anopheles stephensi List. and 30 h for Aedes aegypti (L.). It is limited by cross-reactions which develop between blood group substances as digestion occurs in the stomach of the mosquito. The second system detects the serum protein haptoglobins (Hp) and it is possible to detect the Hp type of blood in single feeds 20 h after feeding for Ae. aegypti and 16 h for A. stephensi. Multiple feeds taken within a short time of each other can be identified up to 16 h after completion of the meal. The minimum amount of blood necessary to effect an identification in a fresh two-part meal is 0·1 mg, which is approximately one-tenth of the total amount of blood taken. It is now therefore possible to measure multiple ‘cryptic meals’ taken from man, if they are of different Hp types. Identification of Hp from A. gambiae sp. A blood-meals has been successfully carried out using material sent from the tropics. Limitations of the techniques as applied to field collections are discussed.

Molecular characterization of the human ABO blood group orthologus system in pigs

2011 ◽  
Vol 42 (3) ◽  
pp. 325-328 ◽  
Author(s):  
D. T. Nguyen ◽  
H. Choi ◽  
H. Jo ◽  
J.-H. Kim ◽  
V. R. Dirisala ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Blood Group ◽  
Abo Blood Group

Characterization of murine monoclonal antibodies againstHelicobacter pylorilipopolysaccharide specific for Lexand Leyblood group determinants

2005 ◽  
Vol 83 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Eleonora Altman ◽  
Blair A Harrison ◽  
Tomoko Hirama ◽  
Vandana Chandan ◽  
Rebecca To ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Monoclonal Antibodies ◽  
Blood Group ◽  
Cell Envelope ◽  
Mucosal Cell ◽  
Blood Group Antigens ◽  
Binding Characteristics ◽  
Igg3 Subclass ◽  
H Pylori

The cell envelope of Helicobacter pylori contains lipopolysaccharide (LPS), the O-chain of which expresses type 2 Lexand Leyblood group antigens, which mimic human gastric mucosal cell-surface glycoconjugates and may contribute to the survival of H. pylori in gastric mucosa. Here we describe the generation of monoclonal antibodies specific for Lexand Leyblood group determinants and the characterization of their binding properties using purified, structurally defined H. pylori LPS, synthetic glycoconjugates, and H. pylori cells. Analysis of oligosaccharide binding by SPR provided a rapid and reliable means for characterization of antibody affinities. One of the antibodies, anti-Lex, was of IgG3 subclass and had superior binding characteristics as compared with the commercially available anti-LexIgM. These antibodies could have potential in the immunodiagnosis of certain types of cancer, in serotyping of H. pylori isolates, and in structure–function studies.Key words: Helicobacter pylori, lipopolysaccharide, monoclonal antibodies, Lewis determinants, immunodiagnosis.

scholarly journals Predictive Modeling of Poor Outcome in Severe COVID-19: A Single-Center Observational Study Based on Clinical, Cytokine and Laboratory Profiles

2021 ◽  
Vol 10 (22) ◽  
pp. 5431
Author(s):  
Óscar Gorgojo-Galindo ◽  
Marta Martín-Fernández ◽  
María Jesús Peñarrubia-Ponce ◽  
Francisco Javier Álvarez ◽  
Christian Ortega-Loubon ◽  
...  
Keyword(s):  
Hospital Admission ◽  
Predictive Model ◽  
Blood Group ◽  
Clinical Laboratory ◽  
Area Under The Curve ◽  
Abo Blood Group ◽  
Poor Outcome ◽  
Emergency Room Admission ◽  
Poor Outcomes

Pneumonia is the main cause of hospital admission in COVID-19 patients. We aimed to perform an extensive characterization of clinical, laboratory, and cytokine profiles in order to identify poor outcomes in COVID-19 patients. Methods: A prospective and consecutive study involving 108 COVID-19 patients was conducted between March and April 2020 at Hospital Clínico Universitario de Valladolid (Spain). Plasma samples from each patient were collected after emergency room admission. Forty-five serum cytokines were measured in duplicate, and clinical data were analyzed using SPPS version 25.0. Results: A multivariate predictive model showed high hepatocyte growth factor (HGF) plasma levels as the only cytokine related to intubation or death risk at hospital admission (OR = 7.38, 95%CI—(1.28–42.4), p = 0.025). There were no comorbidities included in the model except for the ABO blood group, in which the O blood group was associated with a 14-fold lower risk of a poor outcome. Other clinical variables were also included in the predictive model. The predictive model was internally validated by the receiver operating characteristic (ROC) curve with an area under the curve (AUC) of 0.94, a sensitivity of 91.7% and a specificity of 95%. The use of a bootstrapping method confirmed these results. Conclusions: A simple, robust, and quick predictive model, based on the ABO blood group, four common laboratory values, and one specific cytokine (HGF), could be used in order to predict poor outcomes in COVID-19 patients.

scholarly journals Characterization of a novel B(A) allele with BBBA type at the ABO blood group

2006 ◽  
Vol 51 (8) ◽  
pp. 732-736 ◽  
Author(s):  
Zhi-Hui Deng ◽  
Qiong Yu ◽  
Yan-Lian Liang ◽  
Da-Ming Wang ◽  
Yu-Qing Su ◽  
...  
Keyword(s):  
Blood Group ◽  
Abo Blood Group
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