ABO Blood Group Substances from Erythrocytes as �Lipid� Antigens

2015 ◽  
pp. 453-464
Author(s):  
J. Koscielak
Keyword(s):  
Blood Group ◽  
Abo Blood Group ◽  
Lipid Antigens ◽  
Blood Group Substances

Methods for detecting multiple blood-meals in mosquitoes (Diptera, Culicidae)

1976 ◽  
Vol 66 (4) ◽  
pp. 671-679 ◽  
Author(s):  
P. F. L. Boreham ◽  
J. K. Lenahan
Keyword(s):  
Aedes Aegypti ◽  
Blood Group ◽  
Anopheles Stephensi ◽  
Abo Blood Group ◽  
Multiple Blood ◽  
Blood Group Substances ◽  
Multiple Feeding ◽  
The Tropics ◽  
Short Time ◽  
Blood Meals

AbstractTwo techniques have been developed to investigate the incidence of multiple feeding by mosquitoes. One system detects the ABO blood group substances and can be used up to 24 h after feeding in the case of Anopheles stephensi List. and 30 h for Aedes aegypti (L.). It is limited by cross-reactions which develop between blood group substances as digestion occurs in the stomach of the mosquito. The second system detects the serum protein haptoglobins (Hp) and it is possible to detect the Hp type of blood in single feeds 20 h after feeding for Ae. aegypti and 16 h for A. stephensi. Multiple feeds taken within a short time of each other can be identified up to 16 h after completion of the meal. The minimum amount of blood necessary to effect an identification in a fresh two-part meal is 0·1 mg, which is approximately one-tenth of the total amount of blood taken. It is now therefore possible to measure multiple ‘cryptic meals’ taken from man, if they are of different Hp types. Identification of Hp from A. gambiae sp. A blood-meals has been successfully carried out using material sent from the tropics. Limitations of the techniques as applied to field collections are discussed.

scholarly journals ELISA METHOD TO DETECT ABO BLOOD GROUP IN EXTERNAL SECRETION FLUIDS

2019 ◽  
Vol 2 (1) ◽  
pp. 31-38
Author(s):  
Abdul Halim Sadikin ◽  
Yefta Moenadjat ◽  
Novi Sylvia Hardiany
Keyword(s):  
Blood Group ◽  
Positive Reaction ◽  
Enzymatic Reaction ◽  
Abo Blood Group ◽  
Elisa Method ◽  
Blue Colour ◽  
Label Antibody ◽  
Elisa Technique ◽  
Blood Group Substances

Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples.Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique.Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added.Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow.Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.

scholarly journals Serochemical Studies on ABO Blood Group Substances

1955 ◽  
Vol 31 (10) ◽  
pp. 740-744
Author(s):  
Tanemoto FURUHATA ◽  
Kazumichi TSUGE ◽  
Takeshi SUZUKI ◽  
Haruo KURODA ◽  
Akira MATSUYAMA ◽  
...  
Keyword(s):  
Blood Group ◽  
Abo Blood Group ◽  
Blood Group Substances

ABO Blood-group Substances in Gastrointestinal Tract

BMJ ◽  
1957 ◽  
Vol 1 (5018) ◽  
pp. 581-581 ◽  
Author(s):  
C. A. Pasternak ◽  
P. W. Kent
Keyword(s):  
Gastrointestinal Tract ◽  
Blood Group ◽  
Abo Blood Group ◽  
Blood Group Substances

The Correlation Between ABO Blood Group and Neonatal Disease Severity

PEDIATRICS ◽  
2016 ◽  
Vol 137 (Supplement 3) ◽  
pp. 461A-461A
Author(s):  
Kacie E. McMahon ◽  
Jonathan K. Muraskas
Keyword(s):  
Disease Severity ◽  
Blood Group ◽  
Abo Blood Group ◽  
Neonatal Disease
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