A rheo-optical method for the study of aggregated particles: application to the characterization of ABO blood group monoclonal antibodies

2000 ◽  
Vol 39 (6) ◽  
pp. 595-600
Author(s):  
Jean-François Stoltz ◽  
Rodolphos Jose Rasia ◽  
Juana Rosa Valverde ◽  
L. Pla ◽  
Danielle Sontag ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Group ◽  
Optical Method ◽  
Abo Blood Group

Molecular characterization of the human ABO blood group orthologus system in pigs

2011 ◽  
Vol 42 (3) ◽  
pp. 325-328 ◽  
Author(s):  
D. T. Nguyen ◽  
H. Choi ◽  
H. Jo ◽  
J.-H. Kim ◽  
V. R. Dirisala ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Blood Group ◽  
Abo Blood Group

Characterization of murine monoclonal antibodies againstHelicobacter pylorilipopolysaccharide specific for Lexand Leyblood group determinants

2005 ◽  
Vol 83 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Eleonora Altman ◽  
Blair A Harrison ◽  
Tomoko Hirama ◽  
Vandana Chandan ◽  
Rebecca To ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Monoclonal Antibodies ◽  
Blood Group ◽  
Cell Envelope ◽  
Mucosal Cell ◽  
Blood Group Antigens ◽  
Binding Characteristics ◽  
Igg3 Subclass ◽  
H Pylori

The cell envelope of Helicobacter pylori contains lipopolysaccharide (LPS), the O-chain of which expresses type 2 Lexand Leyblood group antigens, which mimic human gastric mucosal cell-surface glycoconjugates and may contribute to the survival of H. pylori in gastric mucosa. Here we describe the generation of monoclonal antibodies specific for Lexand Leyblood group determinants and the characterization of their binding properties using purified, structurally defined H. pylori LPS, synthetic glycoconjugates, and H. pylori cells. Analysis of oligosaccharide binding by SPR provided a rapid and reliable means for characterization of antibody affinities. One of the antibodies, anti-Lex, was of IgG3 subclass and had superior binding characteristics as compared with the commercially available anti-LexIgM. These antibodies could have potential in the immunodiagnosis of certain types of cancer, in serotyping of H. pylori isolates, and in structure–function studies.Key words: Helicobacter pylori, lipopolysaccharide, monoclonal antibodies, Lewis determinants, immunodiagnosis.

scholarly journals Predictive Modeling of Poor Outcome in Severe COVID-19: A Single-Center Observational Study Based on Clinical, Cytokine and Laboratory Profiles

2021 ◽  
Vol 10 (22) ◽  
pp. 5431
Author(s):  
Óscar Gorgojo-Galindo ◽  
Marta Martín-Fernández ◽  
María Jesús Peñarrubia-Ponce ◽  
Francisco Javier Álvarez ◽  
Christian Ortega-Loubon ◽  
...  
Keyword(s):  
Hospital Admission ◽  
Predictive Model ◽  
Blood Group ◽  
Clinical Laboratory ◽  
Area Under The Curve ◽  
Abo Blood Group ◽  
Poor Outcome ◽  
Emergency Room Admission ◽  
Poor Outcomes

Pneumonia is the main cause of hospital admission in COVID-19 patients. We aimed to perform an extensive characterization of clinical, laboratory, and cytokine profiles in order to identify poor outcomes in COVID-19 patients. Methods: A prospective and consecutive study involving 108 COVID-19 patients was conducted between March and April 2020 at Hospital Clínico Universitario de Valladolid (Spain). Plasma samples from each patient were collected after emergency room admission. Forty-five serum cytokines were measured in duplicate, and clinical data were analyzed using SPPS version 25.0. Results: A multivariate predictive model showed high hepatocyte growth factor (HGF) plasma levels as the only cytokine related to intubation or death risk at hospital admission (OR = 7.38, 95%CI—(1.28–42.4), p = 0.025). There were no comorbidities included in the model except for the ABO blood group, in which the O blood group was associated with a 14-fold lower risk of a poor outcome. Other clinical variables were also included in the predictive model. The predictive model was internally validated by the receiver operating characteristic (ROC) curve with an area under the curve (AUC) of 0.94, a sensitivity of 91.7% and a specificity of 95%. The use of a bootstrapping method confirmed these results. Conclusions: A simple, robust, and quick predictive model, based on the ABO blood group, four common laboratory values, and one specific cytokine (HGF), could be used in order to predict poor outcomes in COVID-19 patients.

scholarly journals Characterization of a novel B(A) allele with BBBA type at the ABO blood group

2006 ◽  
Vol 51 (8) ◽  
pp. 732-736 ◽  
Author(s):  
Zhi-Hui Deng ◽  
Qiong Yu ◽  
Yan-Lian Liang ◽  
Da-Ming Wang ◽  
Yu-Qing Su ◽  
...  
Keyword(s):  
Blood Group ◽  
Abo Blood Group

Characterization of a novel O 1 variant allele at the ABO blood group locus

2001 ◽  
Vol 58 (6) ◽  
pp. 422-424 ◽  
Author(s):  
P. Bugert ◽  
L. Rütten ◽  
S. Goerg ◽  
H. Klüter
Keyword(s):  
Blood Group ◽  
Variant Allele ◽  
Abo Blood Group ◽  
Group Locus

scholarly journals Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant.

1983 ◽  
Vol 258 (8) ◽  
pp. 4890-4894 ◽  
Author(s):  
W W Young ◽  
H S Johnson ◽  
Y Tamura ◽  
K A Karlsson ◽  
G Larson ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Group ◽  
Human Blood ◽  
Human Blood Group ◽  
Lewis A

Characterization of the D, c, E and G antigens of the Rh blood group system with human monoclonal antibodies

1988 ◽  
Vol 25 (9) ◽  
pp. 925-930 ◽  
Author(s):  
C. Bloy ◽  
D. Blanchard ◽  
P. Lambin ◽  
D. Goossens ◽  
P. Rouger ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Group ◽  
Blood Group System ◽  
Human Monoclonal Antibodies ◽  
Group System ◽  
Rh Blood Group
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