UV irradiation of interferon-treated chick embryo cells enhances sindbis virus growth

1976 ◽  
Vol 52 (4) ◽  
pp. 351-354
Author(s):  
Y. Umino ◽  
S. Kohno ◽  
S. Saito
Keyword(s):  
Chick Embryo ◽  
Uv Irradiation ◽  
Sindbis Virus ◽  
Virus Growth ◽  
Embryo Cells

Effect of interferon on Sindbis virus growth in chick-embryo cell cultures

1969 ◽  
Vol 15 (6) ◽  
pp. 605-610 ◽  
Author(s):  
Robert B. Stewart ◽  
Edward T. Sheaff
Keyword(s):  
Chick Embryo ◽  
Cell Cultures ◽  
Sindbis Virus ◽  
Embryo Cell ◽  
Virus Growth ◽  
Chick Embryo Cell ◽  
Embryo Cells ◽  
Number Of Cells

A study of the effect of interferon on the growth of Sindbis virus in cultures of chick-embryo cells has shown that interferon forms an association with cells (uptake) and that the action of interferon is concentration rather than amount dependent. Evidence has also been obtained that interferon acts to reduce the yield of virus from cells, but does not reduce the number of cells synthesizing virus (all or none effect).

scholarly journals Inhibition of Virus Growth by Ouabain: Effect of Ouabain on the Growth of HVJ in Chick Embryo Cells

1972 ◽  
Vol 9 (2) ◽  
pp. 234-243 ◽  
Author(s):  
Yoshiyuki Nagai ◽  
Koichiro Maeno ◽  
Masao Iinuma ◽  
Tetsuya Yoshida ◽  
Toshisada Matsumoto
Keyword(s):  
Chick Embryo ◽  
Virus Growth ◽  
Embryo Cells

Interaction of interferon with cells: the relationship of cell-associated interferon and antiviral activity

1970 ◽  
Vol 16 (12) ◽  
pp. 1303-1310 ◽  
Author(s):  
Edward T. Sheaff ◽  
Robert B. Stewart
Keyword(s):  
Chick Embryo ◽  
Antiviral Activity ◽  
Sindbis Virus ◽  
Quantitative Expression ◽  
Embryo Cells ◽  
Relationship Of ◽  
The Relationship

Studies of the effect of interferon on the growth of Sindbis virus in cultures of chick embryo cells have shown that interferon is taken up by cells and that such uptake must occur before antiviral activity is observed. Evidence is presented here that the quantitative expression of antiviral activity is dependent on the amount of interferon that becomes cell associated.

VARIATION IN SENSITIVITY OF AN INTERFERON ASSAY SYSTEM

1967 ◽  
Vol 13 (11) ◽  
pp. 1421-1425
Author(s):  
Robert B. Stewart ◽  
Sunidhkumar S. Gandhi
Keyword(s):  
Chick Embryo ◽  
Cell Cultures ◽  
Dose Response ◽  
Sindbis Virus ◽  
Primary Cultures ◽  
Assay System ◽  
Response Curves ◽  
Challenge Virus ◽  
Embryo Cells ◽  
Altered Sensitivity

Repeated assays of standard preparations of interferon carried out for over a year using primary cultures of chick-embryo cells and Sindbis virus in an assay system showed that cell cultures varied in their sensitivity to interferon. This altered sensitivity was not due to a change in the challenge virus nor to the time of exposure of cells to interferon. An analysis of the data showed that the slope of the dose–response curves remained constant although they could be displaced, indicating changes in sensitivity. Information was also obtained demonstrating that sensitivity of cells to interferon could vary within a single assay.

A substance enhancing virus growth and antagonistic to interferon action

1968 ◽  
Vol 14 (9) ◽  
pp. 965-969 ◽  
Author(s):  
Edward T. Sheaff ◽  
Robert B. Stewart
Keyword(s):  
Chick Embryo ◽  
Cell Line ◽  
Cell Cultures ◽  
Sindbis Virus ◽  
Embryo Cell ◽  
Virus Growth ◽  
Chick Embryo Cell ◽  
Similar Substance

An interferon antagonist was obtained free of both cells and virus from a hamster cell line (BHK-21) infected with Sindbis virus. This material was also found to enhance Sindbis virus growth. A method for the assay of the interferon antagonist is described which is based on its capacity to reduce the activity of interferon as measured by a lack of reduction in Sindbis virus plaque numbers. The antagonist was found to be resistant to ribonuclease and deoxyribonuclease but sensitive to trypsin. Evidence for the existence of a similar substance in Sindbis virus infected chick embryo cell cultures was also obtained.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

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