Effects of temperature and medium composition on the ethanol tolerance ofBacillus stearothermophilus LLD-15

1991 ◽  
Vol 13 (9) ◽  
pp. 627-632 ◽  
Author(s):  
S. A. Amartey ◽  
D. J. Leak ◽  
B. S. Hartley
Keyword(s):  
Ethanol Tolerance ◽  
Medium Composition ◽  
Effects Of Temperature ◽  
Ofbacillus Stearothermophilus

scholarly journals Effects of Temperature, Salinity, and Medium Composition on Compatible Solute Accumulation byThermococcus spp

1998 ◽  
Vol 64 (10) ◽  
pp. 3591-3598 ◽  
Author(s):  
Pedro Lamosa ◽  
Lígia O. Martins ◽  
Milton S. Da Costa ◽  
Helena Santos
Keyword(s):  
Compatible Solutes ◽  
Medium Composition ◽  
Compatible Solute ◽  
Resonance Spectroscopy ◽  
Thermococcus Litoralis ◽  
Solute Accumulation ◽  
Organic Solute ◽  
Low Levels ◽  
Effects Of Temperature ◽  
Thermococcus Celer

ABSTRACT The effects of salinity and growth temperature on the accumulation of intracellular organic solutes were examined by nuclear magnetic resonance spectroscopy (NMR) in Thermococcus litoralis,Thermococcus celer, Thermococcus stetteri, andThermococcus zilligii (strain AN1). In addition, the effects of growth stage and composition of the medium were studied inT. litoralis. A novel compound identified as β-galactopyranosyl-5-hydroxylysine was detected in T. litoralis grown on peptone-containing medium. Besides this newly discovered compound, T. litoralis accumulated mannosylglycerate, aspartate, α-glutamate, di-myo-inositol-1,1′(3,3′)-phosphate, hydroxyproline, and trehalose. The hydroxyproline and β-galactopyranosyl-5-hydroxylysine were probably derived from peptone, while the trehalose was derived from yeast extract; none of these three compounds was detected in the other Thermococcus strains examined. Di-myo-inositol-1,1′(3,3′)-phosphate, aspartate, and mannosylglycerate were detected in T. celer and T. stetteri, and the latter organism also accumulated α-glutamate. The only nonmarine species studied, T. zilligii, accumulated very low levels of α-glutamate and aspartate. The levels of mannosylglycerate and aspartate increased in T. litoralis, T. celer, and T. stetteri in response to salt stress, while di-myo-inositol-1,1′(3,3′)-phosphate was the major intracellular solute at supraoptimal growth temperatures. The phase of growth had a strong influence on the types and levels of compatible solutes in T. litoralis; mannosylglycerate and aspartate were the major solutes during exponential growth, while di-myo-inositol-1,1′(3,3′)-phosphate was the predominant organic solute during the stationary phase of growth. This work revealed an unexpected ability of T. litoralis to scavenge suitable components from the medium and to use them as compatible solutes.

scholarly journals The Effects of Temperature and Culture Medium Composition on Protein Recombinant JSU-pET Expression

2019 ◽  
Vol 17 (2) ◽  
pp. 31
Author(s):  
Indriawati Indriawati ◽  
Slamet Diah Volkandari ◽  
Endang Tri Margawati
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
Culture Medium ◽  
Medium Composition ◽  
Protein Yield ◽  
Culture Temperature ◽  
Factors Influencing ◽  
Effects Of Temperature ◽  
The Right ◽  
Medium Culture

There are several factors influencing the protein expression. The study was aimed to increase the yield of protein recombinant JSU-pET by applying combination of temperature and medium culture composition. This research was composed in a 2x2 factorial design with two level temperatures of 37<sup>o</sup>C and 25<sup>o</sup>C and 2 medium compositions of LB and mLB (modified). <em>Escherichia coli</em> bearing JSU-pET plasmid were grown in two applied media and incubated in two applied temperatures. The cells were harvested by centrifugation the pellets were then lysed. The lysed cells were centrifuged, the supernatant then purified by Ni-NTA Resin Agarose. The purified JSU protein recombinant was characterized and quantified by spectrophotometer. The Result showed that the JSU protein recombinant was successfully expressed on the right size (37kDa) in all treatments even though the JSU-pET showed sharper band in m LB at 37<sup>o</sup>C. Statistically the protein yield was not significant different (P&gt;0.05), however culture temperature of 25<sup>o</sup>C showed higher yield (0.618±0.095 vs 0.704±0.094) than cultured at 37<sup>o</sup>C (0.598±0.137 vs 0.553±0.041), respectively either cultured in LB or mLB. This finding suggests that a lower culture temperature could increase protein yield both either in LB or modified LB.

Improving ethanol tolerance of a self-flocculating yeast by optimization of medium composition

2008 ◽  
Vol 24 (10) ◽  
pp. 2257-2261 ◽  
Author(s):  
Chuang Xue ◽  
Xin-Qing Zhao ◽  
Wen-Jie Yuan ◽  
Feng-Wu Bai
Keyword(s):  
Ethanol Tolerance ◽  
Medium Composition ◽  
Flocculating Yeast

The effects of temperature, incubation atmosphere, and medium composition on arthrospore formation in the fungus Trichophyton mentagrophytes

1979 ◽  
Vol 25 (3) ◽  
pp. 362-366 ◽  
Author(s):  
Ruth G. Emyanitoff ◽  
Tadayo Hashimoto
Keyword(s):  
Germ Tube ◽  
Antifungal Agents ◽  
Trichophyton Mentagrophytes ◽  
Growth Conditions ◽  
Medium Composition ◽  
Partial Replacement ◽  
Atmospheric Conditions ◽  
Effects Of Temperature ◽  
Sublethal Concentrations ◽  
Stimulation Of

Several growth conditions were found to allow abundant arthrospore formation in T. mentagrophytes. These included growth at 32–37 °C on Sabouraud's medium (1% neopeptone, 4% glucose) and growth at temperatures below 32 °C solely on neopeptone or other complex peptide sources without the addition of glucose, a supplementary carbon source. Sabouraud's medium did not allow arthropsore formation at 30 °C under normal atmospheric conditions. However, if oxygen tension were reduced by partial replacement of air with either N2 orCO2 arthrosporulation did occur on Sabouraud's medium at 30 °C. The rate of germ tube elongation was lower under those conditions which supported arthrospore formation, suggesting a correlation between decreased rate of hyphal extension and arthrospore formation. Stimulation of arthrospore formation by sublethal concentrations of several antifungal agents tends to support this hypothesis.

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