Effects of temperature and dilution rate on the copy number of recombinant plasmid in continuous culture ofBacillus stearothermophilus (pLP11)

1985 ◽  
Vol 27 (5) ◽  
pp. 721-728 ◽  
Author(s):  
Jun-ichi Koizumi ◽  
Yoshiaki Monden ◽  
Shuichi Aiba
Keyword(s):  
Continuous Culture ◽  
Dilution Rate ◽  
Copy Number ◽  
Recombinant Plasmid ◽  
Effects Of Temperature ◽  
Ofbacillus Stearothermophilus

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

scholarly journals Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture.

1983 ◽  
Vol 41 (1) ◽  
pp. 74-82 ◽  
Author(s):  
S A Morse ◽  
C S Mintz ◽  
S K Sarafian ◽  
L Bartenstein ◽  
M Bertram ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Serum Resistance

Effect of dilution rate on growth, productivity, cell cycle and size, and shear sensitivity of a hybridoma cell in a continuous culture

1993 ◽  
Vol 41 (4) ◽  
pp. 429-439 ◽  
Author(s):  
D. E. Martens ◽  
C. D. de Gooijer ◽  
C. A. M. van der Velden-de Groot ◽  
E. C. Beuvery ◽  
J. Tramper
Keyword(s):  
Cell Cycle ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Shear Sensitivity
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