An improved method for protoplast formation and its application in the fusion of Rhodotorula rubra with Saccharomyces cerevisiae

1987 ◽  
Vol 148 (1) ◽  
pp. 77-82 ◽  
Author(s):  
C. T. Evans ◽  
D. Conrad
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Improved Method ◽  
Protoplast Formation ◽  
Rhodotorula Rubra

An improved method for whole protein extraction from yeast Saccharomyces cerevisiae

Yeast ◽  
2011 ◽  
Vol 28 (11) ◽  
pp. 795-798 ◽  
Author(s):  
Tingting Zhang ◽  
Jie Lei ◽  
Hanjiang Yang ◽  
Kun Xu ◽  
Rui Wang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Extraction ◽  
Improved Method ◽  
Yeast Saccharomyces Cerevisiae

Ocena oddziaływania na grzyby drożdżopodobne olejku szałwiowego (Oleum Salviae)

2019 ◽  
Vol 20 (3) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alimentary Tract ◽  
Folk Medicine ◽  
Growth Control ◽  
Antimicrobial Properties ◽  
Chemical Compounds ◽  
Geotrichum Candidum ◽  
Rhodotorula Rubra ◽  
Oral Candidosis ◽  
Ancient Times

Introduction. The essential oils were known and used in ancient Times. The oil and extracts of sage are utilized in folk medicine and for food condiment, in cosmetics and perfumes. Sage (Salvia officinalis) belonging to the Labiatae family. Plant grow all over the world. Sage has a number of properties in it antiflogistic and antimicrobial. The oil is used for the treatment of different kind of diseases, including bronchitis, cough, arthritis, rheumatism, ulcers, inflammation of skin, alimentary tract and in Alzheimer’s disease. Etheric oil contain following compounds: α- and β-thujone, 1,8-cineole, camphor, borneol, α-pinene, β-pinene, β-caryophyllene, β-sabinene, limonene, α-humulene, myrcene, α-terpineol, viridiflorol and camphene. The chemical compounds of the oil have antioxidant and antimicrobial properties. Aim. The aim of the dates was to evaluate the susceptibility of yeastlike fungi to sage. Material and methods. A total 30 strains of yeastlike fungi isolated from patients with oral candidosis was tested. The strains were identified with system API 20 C AUX (BioMèrieux), production chlamydospore and pseudohyphe. The susceptibility (MIC) yeastlike fungi to sage oil was determined by means plate dilution technique in Sabouraud’s agar. The suspension contained 105 CFU per spot were spread Steers replicator over the surface of agar containing oil or without sage agar plates (strains growth control). Inoculated agar plates were incubated in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as a lowest concentration of the oil inhibited growth of fungal strains. Results. The results indicated that the sage oil was active against yeastlike fungi in concentrations 0.5-≥ 2.0 mg/ml. The MIC for 66% strains from genus Candida albicans ≥ 2.0 mg/ml. Similarly C. krusei strains were susceptible in range 0.5-≥ 2.0 mg/ml. The oil was less active towards C. glabrata and C. tropicalis strains (MIC 1.0-≥ 2.0 mg/ml). The most susceptible were the strains from genus of C. parapsilosis. The growth was inhibited within the range from 0.5 to 1.0 mg/ml. The strain from genus of Rhodotorula rubra was susceptible on 0.12-0.5 mg/ml and Saccharomyces cerevisiae on 0.25 mg/ml. Conclusions. Sage oil showed antifungal activity. The more susceptible to oil were the strains of Candida parapsilosis, Rhodotorula rubra and Saccharomyces cerevisiae. Oil was less active toward strains Candida glabrata, Candida tropicalis and Geotrichum candidum.

An improved method of xylose utilization by recombinant Saccharomyces cerevisiae

2012 ◽  
Vol 39 (10) ◽  
pp. 1477-1486 ◽  
Author(s):  
Tien-Yang Ma ◽  
Ting-Hsiang Lin ◽  
Teng-Chieh Hsu ◽  
Chiung-Fang Huang ◽  
Gia-Luen Guo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Xylose Utilization ◽  
Improved Method ◽  
Recombinant Saccharomyces Cerevisiae

Isolation and Characterization of Mutants of Saccharomyces cerevisiae Auxotrophic and Conditionally Auxotrophic for 5′ -dTMP

1974 ◽  
Vol 29 (11-12) ◽  
pp. 733-738 ◽  
Author(s):  
Martin Brendel ◽  
Wolfgang W. Fäth
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Complementation Group ◽  
Improved Method ◽  
Genetical Analysis ◽  
Type Locus ◽  
Isolation And Characterization ◽  
Folic Acid Antagonists ◽  
I Gene ◽  
Respiratory Deficient

Abstract An improved method for isolation of yeast m utants auxotrophic for 5′-dTM P is presented. The procedure employs the two folic acid antagonists am inopterin and sulfanilam ide (SAA). Selectiveness of the procedure depends on concentration of SAA and time of incubation. 44 mutants auxotrophic and 3 conditionally auxotrophic for 5′-dTMP were isolated. All belong to one complementation group. The corresponding gene was designated TMP1. Tetrad dissection revealed its chromosomal nature. TMP1 is not closely linked to the genes ADE2,, LEU1, ARG 4, ILV2, HIS5, LYS1 and the mating type locus. With the centromere-linked genes ARG4 and LEU1 I gene TMP1 exhibited second division segregation frequencies of 0.42 and 0.53 respectively, indicative of centromere-linkage. Strains auxotrophic and conditionally auxotrophic for 5′-dTM P were all respiratory deficient (petite). Genetical analysis indicates that the petite phenotype is due to loss of the rho factor in cells harbouring either tmp1 or tmp1ts alleles.

An improved method for lysis of Frankia with acharomopeptidase allows detection of new plasmids

1984 ◽  
Vol 30 (10) ◽  
pp. 1292-1295 ◽  
Author(s):  
Pascal Simonet ◽  
Andre Capellano ◽  
Elisabeth Navarro ◽  
Rene Bardin ◽  
Andre Moiroud
Keyword(s):  
Cell Walls ◽  
Improved Method ◽  
Protoplast Formation ◽  
In Situ Method ◽  
Large Plasmids

An enzyme recently described for Strepiomyces sp. protoplast formation was used to digest Frankia sp. cell walls. This new enzyme, achromopeptidase, allowed the use of an in situ method to detect large plasmids. Plasmids ranging in size from 7 to 190 kilobases were detected in some Frankia strains.

scholarly journals PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae

2013 ◽  
Vol 6 (1) ◽  
Author(s):  
Christine Bonnet ◽  
Céline Rigaud ◽  
Emilie Chanteclaire ◽  
Claire Blandais ◽  
Emilie Tassy-Freches ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Improved Method ◽  
Engineered Saccharomyces Cerevisiae

Improved Method for Ultrastructural Preservation of Saccharomyces cerevisiae JHY-31-11D

1999 ◽  
Vol 7 (1) ◽  
pp. 81-86
Author(s):  
JoAnne Johnson ◽  
Patricia K. Murray ◽  
Robert R. Maronpot
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Improved Method

An improved method for expression and purification of functional human Ca2+ transporter PMCA4b in Saccharomyces cerevisiae

2016 ◽  
Vol 120 ◽  
pp. 51-58 ◽  
Author(s):  
Isaac Corbacho ◽  
Francisco F. García-Prieto ◽  
Ara E. Hinojosa ◽  
María Berrocal ◽  
Ana M. Mata
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Improved Method ◽  
Expression And Purification
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