An improved method for lysis of Frankia with acharomopeptidase allows detection of new plasmids

1984 ◽  
Vol 30 (10) ◽  
pp. 1292-1295 ◽  
Author(s):  
Pascal Simonet ◽  
Andre Capellano ◽  
Elisabeth Navarro ◽  
Rene Bardin ◽  
Andre Moiroud
Keyword(s):  
Cell Walls ◽  
Improved Method ◽  
Protoplast Formation ◽  
In Situ Method ◽  
Large Plasmids

An enzyme recently described for Strepiomyces sp. protoplast formation was used to digest Frankia sp. cell walls. This new enzyme, achromopeptidase, allowed the use of an in situ method to detect large plasmids. Plasmids ranging in size from 7 to 190 kilobases were detected in some Frankia strains.

scholarly journals Application of Furcellaran Nanocomposite Film as Packaging of Cheese

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1428
Author(s):  
Agnieszka Pluta-Kubica ◽  
Ewelina Jamróz ◽  
Gohar Khachatryan ◽  
Adam Florkiewicz ◽  
Pavel Kopel
Keyword(s):  
Silver Nanoparticles ◽  
Nanocomposite Film ◽  
Microbiological Quality ◽  
Inhibitory Effect ◽  
Quality Properties ◽  
In Situ Method ◽  
And Migration ◽  
Total Bacteria

There is a serious need to develop and test new biodegradable packaging which could at least partially replace petroleum-based materials. Therefore, the objective of this work was to examine the influence of the recently developed furcellaran nanocomposite film with silver nanoparticles (obtained by an in situ method) on the quality properties of two cheese varieties: a rennet-curd (gouda) and an acid-curd (quark) cheese. The water content, physicochemical properties, microbiological and organoleptic quality of cheese, and migration of silver nanoparticles were examined. Both the number of Lactococcus and total bacteria count did not differ during storage of gouda regardless of the packaging applied. The number of Lactococcus decreased in analogous quark samples. The use of the film slowed down and inhibited the growth of yeast in gouda and quark, respectively. An inhibitory effect of this film on mold count was also observed; however, only regarding gouda. The level of silver migration was found to be lower in quark than in gouda. The film improved the microbiological quality of cheeses during storage. Consequently, it is worth continuing research for the improvement of this film in order to enable its use in everyday life.

Photocatalytic degradation of tetracycline under visible light using TiO2@sulfur doped carbon nitride nanocomposite synthesized via in-situ method

2021 ◽  
Vol 9 (4) ◽  
pp. 105560
Author(s):  
Krishnan Divakaran ◽  
Amanulla Baishnisha ◽  
Vellaichamy Balakumar ◽  
Krishnan Nattamai Perumal ◽  
Chandran Meenakshi ◽  
...  
Keyword(s):  
Visible Light ◽  
Photocatalytic Degradation ◽  
Carbon Nitride ◽  
In Situ Method

Measuring Reservoir Compaction Through Radioactive Marker Technique

2002 ◽  
Vol 124 (4) ◽  
pp. 269-275
Author(s):  
Paolo Macini ◽  
Ezio Mesini
Keyword(s):  
Land Subsidence ◽  
Reservoir Rock ◽  
Reservoir Compaction ◽  
Critical Comparison ◽  
In Situ Method ◽  
Sufficient Precision ◽  
Good Agreement

Radioactive Marker Technique (RMT), an in-situ method to measure reservoir rock compaction and to evaluate uniaxial compressibility coefficients Cm, is examined here. Recent field applications seems to confirm that RMT-derived Cm’s match with sufficient precision with those calculated from land subsidence observed over the field by means of geodetic surveys, but are not always in good agreement with those derived from lab measurements. In particular, here is reported an application of RMT in the Italian Adriatic offshore, which highlights the discrepancies of Cm’s measurements from lab and RMT. At present, these discrepancies aren’t thoroughly understood, so, from an applicative standpoint, it is still necessary to perform a critical comparison and integration between both set of data.

Control of Hierarchically Ordered Positive and Negative Replicas of Wood Cellular Structures by Surfactant-Directed Sol-Gel Mineralization

2002 ◽  
Vol 726 ◽  
Author(s):  
Yongsoon Shin ◽  
Jun Liu ◽  
Li-Qiong Wang ◽  
Jeong Ho Chang ◽  
William D. Samuels ◽  
...  
Keyword(s):  
Silicic Acid ◽  
Cell Walls ◽  
Cellular Structure ◽  
Sol Gel ◽  
Ceramic Materials ◽  
Silica Particles ◽  
Low Ph ◽  
Cellular Structures ◽  
Inner Surface

AbstractWe here report the synthesis of ordered ceramic materials with hierarchy produced by an in-situ mineralization of ordered wood cellular structures with surfactant-templated sol-gel at different pH. At low pH, a silicic acid is coated onto inner surface of wood cellular structure and it penetrates into pores left, where degraded lignin and hemicellulose are leached out, to form a positive replica, while at high pH the precipitating silica particles due to fast condensation clog the cells and pit structures to form a negative replica of wood. The calcined monoliths produced in different pHs contain ordered wood cellular structures, multi-layered cell walls, pits, vessels well-preserved with positive or negative contrasts, respectively. The surfactant-templated mineralization produces ordered hexagonal nanopores with 20Å in the cell walls after calcination.

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