scholarly journals Παραγωγή του yeast killer factor από διάφορα είδη candida torulopsis glabrata saccharomyces cerevisiae και rhodotorula rubra

1984 ◽  
Author(s):  
Ευάγγελος Πολυχρονόπουλος
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rhodotorula Rubra ◽  
Torulopsis Glabrata ◽  
Yeast Killer

Surface Structures in Budding Cells of Torulopsis Giabrata

1969 ◽  
Vol 27 ◽  
pp. 390-391
Author(s):  
Mercedes R. Edwards ◽  
Stanley Holt
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fine Structure ◽  
Yeast Species ◽  
Surface Structures ◽  
Logarithmic Phase ◽  
Torulopsis Glabrata ◽  
Conventional Methods

The fine structure of Saccharomyces cerevisiae has been investigated extensively while relatively little work has been done on other yeast species. The present study compares Torulopsis glabrata with S. cerevisiae. Cells in the logarithmic phase of growth were (a) quickly frozen in Freon 22, cut and replicated in a Balzer's apparatus according to Moor's technique; (b) fixed in glutaraldehyde-osmium, dehydrated, and embedded by conventional methods. Only the illustrations of the surface structures are presented.

Ocena oddziaływania na grzyby drożdżopodobne olejku szałwiowego (Oleum Salviae)

2019 ◽  
Vol 20 (3) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alimentary Tract ◽  
Folk Medicine ◽  
Growth Control ◽  
Antimicrobial Properties ◽  
Chemical Compounds ◽  
Geotrichum Candidum ◽  
Rhodotorula Rubra ◽  
Oral Candidosis ◽  
Ancient Times

Introduction. The essential oils were known and used in ancient Times. The oil and extracts of sage are utilized in folk medicine and for food condiment, in cosmetics and perfumes. Sage (Salvia officinalis) belonging to the Labiatae family. Plant grow all over the world. Sage has a number of properties in it antiflogistic and antimicrobial. The oil is used for the treatment of different kind of diseases, including bronchitis, cough, arthritis, rheumatism, ulcers, inflammation of skin, alimentary tract and in Alzheimer’s disease. Etheric oil contain following compounds: α- and β-thujone, 1,8-cineole, camphor, borneol, α-pinene, β-pinene, β-caryophyllene, β-sabinene, limonene, α-humulene, myrcene, α-terpineol, viridiflorol and camphene. The chemical compounds of the oil have antioxidant and antimicrobial properties. Aim. The aim of the dates was to evaluate the susceptibility of yeastlike fungi to sage. Material and methods. A total 30 strains of yeastlike fungi isolated from patients with oral candidosis was tested. The strains were identified with system API 20 C AUX (BioMèrieux), production chlamydospore and pseudohyphe. The susceptibility (MIC) yeastlike fungi to sage oil was determined by means plate dilution technique in Sabouraud’s agar. The suspension contained 105 CFU per spot were spread Steers replicator over the surface of agar containing oil or without sage agar plates (strains growth control). Inoculated agar plates were incubated in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as a lowest concentration of the oil inhibited growth of fungal strains. Results. The results indicated that the sage oil was active against yeastlike fungi in concentrations 0.5-≥ 2.0 mg/ml. The MIC for 66% strains from genus Candida albicans ≥ 2.0 mg/ml. Similarly C. krusei strains were susceptible in range 0.5-≥ 2.0 mg/ml. The oil was less active towards C. glabrata and C. tropicalis strains (MIC 1.0-≥ 2.0 mg/ml). The most susceptible were the strains from genus of C. parapsilosis. The growth was inhibited within the range from 0.5 to 1.0 mg/ml. The strain from genus of Rhodotorula rubra was susceptible on 0.12-0.5 mg/ml and Saccharomyces cerevisiae on 0.25 mg/ml. Conclusions. Sage oil showed antifungal activity. The more susceptible to oil were the strains of Candida parapsilosis, Rhodotorula rubra and Saccharomyces cerevisiae. Oil was less active toward strains Candida glabrata, Candida tropicalis and Geotrichum candidum.

Radioimmunoassay for yeast killer toxin from Saccharomyces cerevisiae

1981 ◽  
Vol 27 (8) ◽  
pp. 847-849
Author(s):  
Farooq A. Siddiqui ◽  
Howard Bussey
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ammonium Sulfate ◽  
Column Chromatography ◽  
Specific Activity ◽  
Killer Toxin ◽  
Ammonium Sulfate Precipitation ◽  
Antibody Preparation ◽  
Precipitation Band ◽  
Yeast Killer ◽  
Yeast Killer Toxin

Aradioimmunoassay was developed for the K1 killer toxin from strain T158C/S14a of Saccharomyces cerevisiae. 125I-labeled toxin was made to a specific activity of 100 μCi/mg of protein (1 μCi = 37 kBq). Antibody to purified toxin was prepared in rabbits using toxin cross-linked to itself. These antibodies, partially purified by 50% ammonium sulfate precipitation and Sepharose CL-6B column chromatography, produced one precipitation band with killer toxin and bound 125I-labeled toxin in a radioimmunoassay. The antibody preparation also bound with the toxins from another K1 killer, A364A, and three chromosomal superkiller mutants derived from it.

scholarly journals Killer toxin of Saccharomyces cerevisiae Y500-4L active against Fleischmann and Itaiquara commercial brands of yeast

1999 ◽  
Vol 30 (3) ◽  
pp. 253-257 ◽  
Author(s):  
Giselle A.M. Soares ◽  
Hélia H. Sato
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Elevated Temperatures ◽  
Killer Toxin ◽  
Toxin Production ◽  
Chromosomal Dna ◽  
Killer Activity ◽  
Killer Yeast ◽  
Torulopsis Glabrata ◽  
Hansenula Anomala ◽  
Killer Yeasts

The strain Saccharomyces cerevisiae Y500-4L, previously selected from the must of alcohol producing plants and showing high fermentative and killer capacities, was characterized according to the interactions between the yeasts and examined for curing and detection of dsRNA plasmids, which code for the killer character. The killer yeast S. cerevisiae Y500-4L showed considerable killer activity against the Fleischmann and Itaiquara commercial brands of yeast and also against the standard killer yeasts K2 (S. diastaticus NCYC 713), K4 (Candida glabrata NCYC 388) and K11 (Torulopsis glabrata ATCC 15126). However S. cerevisiae Y500-4L showed sensitivity to the killer toxin produced by the standard killer yeasts K8 (Hansenula anomala NCYC 435), K9 (Hansenula mrakii NCYC 500), K10 (Kluyveromyces drosophilarum NCYC 575) and K11 (Torulopsis glabrata ATCC 15126). No M-dsRNA plasmid was detected in the S. cerevisiae Y500-4L strain and these results suggest that the genetic basis for toxin production is encoded by chromosomal DNA. The strain S. cerevisiae Y500-4L was more resistant to the loss of the phenotype killer with cycloheximide and incubation at elevated temperatures (40oC) than the standard killer yeast S. cerevisiae K1.

scholarly journals Effect of yeast killer toxin on sensitive cells of Saccharomyces cerevisiae.

1981 ◽  
Vol 256 (20) ◽  
pp. 10420-10425
Author(s):  
P. de la Peña ◽  
F. Barros ◽  
S. Gascón ◽  
P.S. Lazo ◽  
S. Ramos
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Killer Toxin ◽  
Yeast Killer ◽  
Yeast Killer Toxin
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