Imaging the lamellipodium of migrating epithelial cells in vivo by atomic force microscopy

1993 ◽  
Vol 425 (5-6) ◽  
pp. 506-510 ◽  
Author(s):  
Hans Oberleithner ◽  
Gerhard Giebisch ◽  
John Geibel
Keyword(s):  
Atomic Force Microscopy ◽  
Epithelial Cells ◽  
Force Microscopy ◽  
Atomic Force

Diabetes increases stiffness of live cardiomyocytes measured by atomic force microscopy nanoindentation

2014 ◽  
Vol 307 (10) ◽  
pp. C910-C919 ◽  
Author(s):  
Juan C. Benech ◽  
Nicolás Benech ◽  
Ana I. Zambrana ◽  
Inés Rauschert ◽  
Verónica Bervejillo ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Physiological Condition ◽  
Diabetic Mice ◽  
Cell Nuclei ◽  
Buffer Solution ◽  
Isolated Cardiomyocytes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Myocardial Fibers

Stiffness of live cardiomyocytes isolated from control and diabetic mice was measured using the atomic force microscopy nanoindentation method. Type 1 diabetes was induced in mice by streptozotocin administration. Histological images of myocardium from mice that were diabetic for 3 mo showed disorderly lineup of myocardial cells, irregularly sized cell nuclei, and fragmented and disordered myocardial fibers with interstitial collagen accumulation. Phalloidin-stained cardiomyocytes isolated from diabetic mice showed altered (i.e., more irregular and diffuse) actin filament organization compared with cardiomyocytes from control mice. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) pump expression was reduced in homogenates obtained from the left ventricle of diabetic animals compared with age-matched controls. The apparent elastic modulus (AEM) for live control or diabetic isolated cardiomyocytes was measured using the atomic force microscopy nanoindentation method in Tyrode buffer solution containing 1.8 mM Ca2+ and 5.4 mM KCl (physiological condition), 100 nM Ca2+ and 5.4 mM KCl (low extracellular Ca2+ condition), or 1.8 mM Ca2+ and 140 mM KCl (contraction condition). In the physiological condition, the mean AEM was 112% higher for live diabetic than control isolated cardiomyocytes (91 ± 14 vs. 43 ± 7 kPa). The AEM was also significantly higher in diabetic than control cardiomyocytes in the low extracellular Ca2+ and contraction conditions. These findings suggest that the material properties of live cardiomyocytes were affected by diabetes, resulting in stiffer cells, which very likely contribute to high diastolic LV stiffness, which has been observed in vivo in some diabetes mellitus patients.

Living Renal Epithelial Cells Imaged by Atomic Force Microscopy

Nephron ◽  
1994 ◽  
Vol 66 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Hans Oberleithner ◽  
Albrecht Schwab ◽  
Wenhui Wang ◽  
Gerhard Giebisch ◽  
Forest Hume ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Epithelial Cells ◽  
Renal Epithelial Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy

Microscopy ◽  
2017 ◽  
Vol 66 (4) ◽  
pp. 272-282 ◽  
Author(s):  
Yanshu Zhang ◽  
Aiko Yoshida ◽  
Nobuaki Sakai ◽  
Yoshitsugu Uekusa ◽  
Masahiro Kumeta ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Actin Network ◽  
Cortical Actin ◽  
Force Microscopy ◽  
Atomic Force

Alkaline Stress-Induced Cell Transformation

Physiology ◽  
1994 ◽  
Vol 9 (3) ◽  
pp. 165-168
Author(s):  
H Oberleithner ◽  
A Schwab
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Transformation ◽  
Alkaline Stress ◽  
Membrane Turnover ◽  
Force Microscopy ◽  
Cells In Culture ◽  
Atomic Force

Sustained alkalosis transforms epithelial cells in culture. Genotypically altered cells express an endogenous Ca2+ oscillator that probably is the motor for restless locomotion of these cells. Atomic force microscopy discloses membrane turnover processes during cell migration at the nanometer level.

Alkaline Stress-Induced Cell Transformation

Physiology ◽  
1994 ◽  
Vol 9 (4) ◽  
pp. 165-168
Author(s):  
Hans Oberleithner ◽  
Albrecht Schwab
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Transformation ◽  
Alkaline Stress ◽  
Membrane Turnover ◽  
Force Microscopy ◽  
Cells In Culture ◽  
Atomic Force

Sustained alkalosis transforms epithelial cells in culture. Genotypically altered cells express an endogenous Ca2+ oscillator that probably is the motor for restless locomotion of these cells. Atomic force microscopy discloses membrane turnover processes during cell migration at the nanometer level.

In Vivo Atomic Force Microscopy of Surface Proteins onDeinococcus radiodurans

Langmuir ◽  
2001 ◽  
Vol 17 (9) ◽  
pp. 2624-2628 ◽  
Author(s):  
T. E. Lister ◽  
P. J. Pinhero
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Proteins ◽  
Force Microscopy ◽  
Atomic Force

In vivo nanomechanical imaging of blood-vessel tissues directly in living mammals using atomic force microscopy

2009 ◽  
Vol 95 (1) ◽  
pp. 013704 ◽  
Author(s):  
Youdong Mao ◽  
Quanmei Sun ◽  
Xiufeng Wang ◽  
Qi Ouyang ◽  
Li Han ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Vessel ◽  
Force Microscopy ◽  
Atomic Force
Sign in / Sign up

Export Citation Format

Share Document