scholarly journals In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy

Microscopy ◽  
2017 ◽  
Vol 66 (4) ◽  
pp. 272-282 ◽  
Author(s):  
Yanshu Zhang ◽  
Aiko Yoshida ◽  
Nobuaki Sakai ◽  
Yoshitsugu Uekusa ◽  
Masahiro Kumeta ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Actin Network ◽  
Cortical Actin ◽  
Force Microscopy ◽  
Atomic Force

Diabetes increases stiffness of live cardiomyocytes measured by atomic force microscopy nanoindentation

2014 ◽  
Vol 307 (10) ◽  
pp. C910-C919 ◽  
Author(s):  
Juan C. Benech ◽  
Nicolás Benech ◽  
Ana I. Zambrana ◽  
Inés Rauschert ◽  
Verónica Bervejillo ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Physiological Condition ◽  
Diabetic Mice ◽  
Cell Nuclei ◽  
Buffer Solution ◽  
Isolated Cardiomyocytes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Myocardial Fibers

Stiffness of live cardiomyocytes isolated from control and diabetic mice was measured using the atomic force microscopy nanoindentation method. Type 1 diabetes was induced in mice by streptozotocin administration. Histological images of myocardium from mice that were diabetic for 3 mo showed disorderly lineup of myocardial cells, irregularly sized cell nuclei, and fragmented and disordered myocardial fibers with interstitial collagen accumulation. Phalloidin-stained cardiomyocytes isolated from diabetic mice showed altered (i.e., more irregular and diffuse) actin filament organization compared with cardiomyocytes from control mice. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) pump expression was reduced in homogenates obtained from the left ventricle of diabetic animals compared with age-matched controls. The apparent elastic modulus (AEM) for live control or diabetic isolated cardiomyocytes was measured using the atomic force microscopy nanoindentation method in Tyrode buffer solution containing 1.8 mM Ca2+ and 5.4 mM KCl (physiological condition), 100 nM Ca2+ and 5.4 mM KCl (low extracellular Ca2+ condition), or 1.8 mM Ca2+ and 140 mM KCl (contraction condition). In the physiological condition, the mean AEM was 112% higher for live diabetic than control isolated cardiomyocytes (91 ± 14 vs. 43 ± 7 kPa). The AEM was also significantly higher in diabetic than control cardiomyocytes in the low extracellular Ca2+ and contraction conditions. These findings suggest that the material properties of live cardiomyocytes were affected by diabetes, resulting in stiffer cells, which very likely contribute to high diastolic LV stiffness, which has been observed in vivo in some diabetes mellitus patients.

In Vivo Atomic Force Microscopy of Surface Proteins onDeinococcus radiodurans

Langmuir ◽  
2001 ◽  
Vol 17 (9) ◽  
pp. 2624-2628 ◽  
Author(s):  
T. E. Lister ◽  
P. J. Pinhero
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Proteins ◽  
Force Microscopy ◽  
Atomic Force

In vivo nanomechanical imaging of blood-vessel tissues directly in living mammals using atomic force microscopy

2009 ◽  
Vol 95 (1) ◽  
pp. 013704 ◽  
Author(s):  
Youdong Mao ◽  
Quanmei Sun ◽  
Xiufeng Wang ◽  
Qi Ouyang ◽  
Li Han ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Vessel ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Changes in Skin Barrier Function after Repeated Exposition to Phospholipid-Based Surfactants and Sodium Dodecyl Sulfate In Vivo and Corneocyte Surface Analysis by Atomic Force Microscopy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 436
Author(s):  
Claudia Vater ◽  
Alexandra Apanovic ◽  
Christoph Riethmüller ◽  
Brigitte Litschauer ◽  
Michael Wolzt ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sodium Dodecyl ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Barrier Function ◽  
Water Control ◽  
Force Microscopy ◽  
Atomic Force ◽  
Forearm Skin

(1) Background: The aim of the study was to evaluate the effect of pure lecithins in comparison to a conventional surfactant on skin in vivo. (2) Methods: Physiological skin parameters were evaluated at the beginning and the end of the study (day 1 and day 4) (n = 8, healthy forearm skin) with an Aquaflux®, skin-pH-Meter, Corneometer® and an Epsilon® sensor. Confocal Raman spectroscopy was employed to monitor natural moisturizing factor, urea and water content of the participants’ skin. Tape strips of treated skin sites were taken and the collected corneocytes were subjected to atomic force microscopy. Circular nano objects were counted, and dermal texture indices were determined. (3) Results: Transepidermal water loss was increased, and skin hydration was decreased after treatment with SDS and LPC80. Natural moisturizing factor and urea concentrations within the outermost 10 µm of the stratum corneum were lower than after treatment with S75 or water. Dermal texture indices of skin treated with SDS were higher than skin treated with water (control). (4) Conclusions: Results suggest very good (S75) or good (LPC80) skin-tolerability of lecithin-based surfactants in comparison to SDS and encourage further investigation.

Investigation of the influence of phenosan, ichphan-10, and melafen on red blood cells in vivo by atomic force microscopy

2011 ◽  
Vol 441 (1) ◽  
pp. 245-247 ◽  
Author(s):  
V. I. Binyukov ◽  
O. M. Alekseeva ◽  
E. M. Mil ◽  
A. A. Albantova ◽  
S. G. Fattachov ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Mobility of nucleosomes is regulated by their binding partners

2022 ◽  
Author(s):  
Daniel P Melters ◽  
Keir C Neuman ◽  
Tatini Rakshit ◽  
Yamini Dalal
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
High Speed ◽  
Diffusion Constant ◽  
Chromatin Accessibility ◽  
Chromatin Dynamics ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force

Chromatin accessibility is modulated in a variety of ways, both to create open and closed chromatin states which are critical for eukaryotic gene regulation. At the mechanistic single molecule level, how accessibility is regulated remains a fundamental question in the field. Here, we use single molecule tracking by high-speed atomic force microscopy to investigate this question using chromatin arrays and extend our findings into the nucleus. By high-speed atomic force microscopy, we tracked chromatin dynamics in real time and observed that the essential kinetochore protein CENP-C reduces the diffusion constant of CENP-A nucleosomes and the linker H1.5 protein restricts H3 nucleosome mobility. We subsequently interrogated how CENP-C modulates CENP-A chromatin dynamics in vivo. Overexpressing CENP-C resulted in reduced centromeric transcription and impaired loading of new CENP-A molecules. These data suggest a model in which inner kinetochore proteins are critically involved in modulating chromatin accessibility and consequently, noncoding transcription at human centromeres.

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