Living Renal Epithelial Cells Imaged by Atomic Force Microscopy

Nephron ◽  
1994 ◽  
Vol 66 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Hans Oberleithner ◽  
Albrecht Schwab ◽  
Wenhui Wang ◽  
Gerhard Giebisch ◽  
Forest Hume ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Epithelial Cells ◽  
Renal Epithelial Cells ◽  
Force Microscopy ◽  
Atomic Force

Alkaline Stress-Induced Cell Transformation

Physiology ◽  
1994 ◽  
Vol 9 (3) ◽  
pp. 165-168
Author(s):  
H Oberleithner ◽  
A Schwab
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Transformation ◽  
Alkaline Stress ◽  
Membrane Turnover ◽  
Force Microscopy ◽  
Cells In Culture ◽  
Atomic Force

Sustained alkalosis transforms epithelial cells in culture. Genotypically altered cells express an endogenous Ca2+ oscillator that probably is the motor for restless locomotion of these cells. Atomic force microscopy discloses membrane turnover processes during cell migration at the nanometer level.

Alkaline Stress-Induced Cell Transformation

Physiology ◽  
1994 ◽  
Vol 9 (4) ◽  
pp. 165-168
Author(s):  
Hans Oberleithner ◽  
Albrecht Schwab
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Transformation ◽  
Alkaline Stress ◽  
Membrane Turnover ◽  
Force Microscopy ◽  
Cells In Culture ◽  
Atomic Force

Sustained alkalosis transforms epithelial cells in culture. Genotypically altered cells express an endogenous Ca2+ oscillator that probably is the motor for restless locomotion of these cells. Atomic force microscopy discloses membrane turnover processes during cell migration at the nanometer level.

scholarly journals Observations of the Effects of Angiotensin II Receptor Blocker on Angiotensin II-Induced Morphological and Mechanical Changes in Renal Tubular Epithelial Cells Using Atomic Force Microscopy

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Jin Sug Kim ◽  
Gi-Ja Lee ◽  
Tae Won Lee ◽  
Chun Gyoo Ihm ◽  
Yu Ho Lee ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Angiotensin Ii ◽  
Epithelial Cells ◽  
Cell Surface ◽  
Angiotensin Ii Receptor Blocker ◽  
Ang Ii ◽  
Tubular Epithelial Cells ◽  
Force Microscopy ◽  
Atomic Force

Objective. Angiotensin II (Ang II) plays a profibrotic role in the kidneys. Although many pathways of Ang II have been discovered, the morphological and mechanical aspects have not been well investigated. We observed the changes in tubular epithelial cells (TECs) after Ang II treatment with or without Ang II receptor blockers (ARBs) using atomic force microscopy (AFM). Methods. TECs were stimulated with Ang II with or without telmisartan, PD123319, and blebbistatin. AFM was performed to measure the cellular stiffness, cell volume, and cell surface roughness. Epithelial to mesenchymal transition markers were determined via immunocytochemistry. Results. After Ang II stimulation, cells transformed to a flattened and elongated mesenchymal morphology. Cell surface roughness and volume significantly increased in Ang II treated TECs. Ang II also induced an increase in phospho-myosin light chain and F-actin and a decrease in E-cadherin. Ang II coincubation with either telmisartan or blebbistatin attenuated these Ang II-induced changes. Conclusion. We report, for the first time, the use of AFM in directly observing the changes in TECs after Ang II treatment with or without ARBs. Simultaneously, we successfully measured the selective effect of PD123319 or blebbistatin. AFM could be a noninvasive evaluating strategy for cellular processes in TECs.

scholarly journals Mechanical Characterization of Human Corneal Epithelial Cells using Atomic Force Microscopy

2021 ◽  
Author(s):  
Wenyan Yu ◽  
Tong Liu ◽  
Jianqing Mi ◽  
hyock ju Kwon
Keyword(s):  
Mechanical Properties ◽  
Mechanical Property ◽  
Atomic Force Microscopy ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Force Microscopy ◽  
Nano Indentation ◽  
Atomic Force ◽  
Human Corneal Epithelial Cells

Abstract Background Cell mechanics focuses on the mechanical properties of the cells and how they affect biological behaviors of cells. Mechanical property change of cells can reflect specific diseases and conditions. Therefore, detecting the changes in cell mechanical properties can potentially be associated with diagnosis and treatment of diseases.MethodsMechanical properties of living and dead human corneal epithelial cells (HCECs) were investigated with nano-indentation method using atomic force microscopy (AFM). Sneddon model was adopted to analyze the contacts between pyramidal AFM tip and soft materials. Polyacrylamide (PAAm) substrates with different stiffness were fabricated and human corneal epithelial cells (HCECs) were cultured on them. By applying nano-indentation to HCECs, we examined the distribution of elasticity across cells, and studied the differences between living and dead cells. Findings The central part of a cell, closer to nucleus, was more compliant than the outer regions, toward the surface of the cell’s lipid bilayer. Also, HCECs became stiffer as the stiffness of the substrate increased. Moreover, it was found that dead cells have much higher elastic moduli and exhibited elastic behavior, while living cells showed highly viscoelastic behavior.InterpretationAFM probing is an effective tool for characterizing cell mechanical behavior. More indentation results should be made in further studies to form statistical model of elasticity distribution for HCECs. This study can be extended to investigate the mechanical behaviors of benign and malignant cells or drug interactions on cells, which can establish more probable mechanical property–disease connections.

scholarly journals Mechanical properties of the surface of gastric epithelial cells in patients with oncologic pathology

2021 ◽  
pp. 152-159
Author(s):  
A. S. Shaforost ◽  
E. V. Voropaev ◽  
S. L. Achinovich ◽  
R. A. Silin
Keyword(s):  
Experimental Data ◽  
Gastric Cancer ◽  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Epithelial Cells ◽  
Tumor Cells ◽  
Cortical Layer ◽  
Gastric Epithelial Cells ◽  
Force Microscopy ◽  
Atomic Force

Objective: to study the efficiency of the application of atomic force microscopy for the identification of differences in the structural and mechanical properties of gastric epithelial cells in normal conditions and in gastric cancer.Material and methods. The structural and mechanical properties of the membrane of the epithelial cells of the gastric mucosa in 7 patients with gastric cancer were assessed using atomic force microscopy in contact mode.Results. The comparison of the size and shape of gastric mucous cells (GLC), their healthy and tumor areas has showed that in both the cases they have a slightly elongated rounded shape and similar dimensions: length × width × height of the cell is norm (in μm): 7.5 × 6.8 × 0.6, and in case of oncologic pathology (in μm): 7.2 × 6.9 × 1.0. Tumor cells are characterized by a 13% increase in their surface roughness compared to normal epithelial cells. The lateral force microscopy method allows of obtaining data on the structure of the submembrane cortical layer of the cell cytoskeleton. According to experimental data, a 2.48-fold decrease in the value of the friction force (Fl) in epithelial cells from healthy areas of the coolant indicates increased density and integrity of the cortical layer of the cytoskeleton in normal conditions compared to those of tumor cells.Conclusion. The analysis of the experimental data has found that tumor cells from the gastric mucosa differ from healthy cells in terms of their nanomechanical properties: they are characterized by a rougher surface, which consists of fewer structural elements of a larger size. The work has showed the interrelation of the indicators of the frictional properties of the cell surface: the values of the friction forces and the roughness of the friction map.

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