scholarly journals An in vivo study of electrical charge distribution on the bacterial cell wall by atomic force microscopy in vibrating force mode

Nanoscale ◽  
2015 ◽  
Vol 7 (19) ◽  
pp. 8843-8857 ◽  
Author(s):  
Christian Marlière ◽  
Samia Dhahri
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Charge Distribution ◽  
Bacterial Cell ◽  
Bacterial Cell Wall ◽  
Electrical Charge ◽  
Force Microscopy ◽  
Atomic Force ◽  
Force Mode

Probing Chemical and Physical Properties of Poplar Tension Wood Using Confocal Raman Microscopy and Pulsed Force Mode Atomic Force Microscopy

MRS Advances ◽  
2017 ◽  
Vol 2 (19-20) ◽  
pp. 1103-1109
Author(s):  
Mikhael Soliman ◽  
Laurene Tetard
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Plant Cell Wall ◽  
Cellulose Content ◽  
Force Microscopy ◽  
Atomic Force ◽  
Fundamental Understanding ◽  
Force Mode ◽  
Confocal Raman ◽  
Mode Atomic Force Microscopy

ABSTRACTLignocellulosic biofuels have been identified as a possible solution to contribute to the world’s demands in energy and environmental sustainability. However, the fundamental understanding of the physical and chemical traits hindering key reactions during biomass to biofuel conversion processes has been limited by the lack of suitable tools and by the large natural variability in such systems. Reaction wood constitutes a good model system to study variations of cellulose content, given the increase in cellulose content in the cell walls of the region under tension in the plant during growth. In this work, we use confocal Raman mapping and Pulsed Force Mode Atomic Force Microscopy (PFM) to explore the effect of variation in cellulose content on the structure and composition of the plant cell wall at the nanoscale. Using statistical analysis on Raman datasets, the characteristic peaks for cellulose and lignin are examined to reveal changes in peak positions across the different scanned regions of the cross section. PFM is used to study local mechanical properties of the different layers of the cell wall. Our approach facilitates the correlation of structure-composition traits of the plant cell wall for a more fundamental understanding of processes involved in biofuel research.

scholarly journals In vivo atomic force microscopy–infrared spectroscopy of bacteria

2018 ◽  
Vol 15 (140) ◽  
pp. 20180115 ◽  
Author(s):  
Kamila Kochan ◽  
David Perez-Guaita ◽  
Julia Pissang ◽  
Jhih-Hang Jiang ◽  
Anton Y. Peleg ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Single Cells ◽  
Bacterial Species ◽  
Molecular Changes ◽  
Cell Wall Components ◽  
Force Microscopy ◽  
Atomic Force ◽  
Wall Components

A new experimental platform for probing nanoscale molecular changes in living bacteria using atomic force microscopy–infrared (AFM–IR) spectroscopy is demonstrated. This near-field technique is eminently suited to the study of single bacterial cells. Here, we report its application to monitor dynamical changes occurring in the cell wall during cell division in Staphylococcus aureus using AFM to demonstrate the division of the cell and AFM–IR to record spectra showing the thickening of the septum . This work was followed by an investigation into single cells, with particular emphasis on cell-wall signatures, in several bacterial species. Specifically, mainly cell wall components from S. aureus and Escherichia coli containing complex carbohydrate and phosphodiester groups, including peptidoglycans and teichoic acid, could be identified and mapped at nanometre spatial resolution. Principal component analysis of AFM–IR spectra of six living bacterial species enabled the discrimination of Gram-positive from Gram-negative bacteria based on spectral bands originating mainly from the cell wall components. The ability to monitor in vivo molecular changes during cellular processes in bacteria at the nanoscale opens a new platform to study environmental influences and other factors that affect bacterial chemistry.

Diabetes increases stiffness of live cardiomyocytes measured by atomic force microscopy nanoindentation

2014 ◽  
Vol 307 (10) ◽  
pp. C910-C919 ◽  
Author(s):  
Juan C. Benech ◽  
Nicolás Benech ◽  
Ana I. Zambrana ◽  
Inés Rauschert ◽  
Verónica Bervejillo ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Physiological Condition ◽  
Diabetic Mice ◽  
Cell Nuclei ◽  
Buffer Solution ◽  
Isolated Cardiomyocytes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Myocardial Fibers

Stiffness of live cardiomyocytes isolated from control and diabetic mice was measured using the atomic force microscopy nanoindentation method. Type 1 diabetes was induced in mice by streptozotocin administration. Histological images of myocardium from mice that were diabetic for 3 mo showed disorderly lineup of myocardial cells, irregularly sized cell nuclei, and fragmented and disordered myocardial fibers with interstitial collagen accumulation. Phalloidin-stained cardiomyocytes isolated from diabetic mice showed altered (i.e., more irregular and diffuse) actin filament organization compared with cardiomyocytes from control mice. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) pump expression was reduced in homogenates obtained from the left ventricle of diabetic animals compared with age-matched controls. The apparent elastic modulus (AEM) for live control or diabetic isolated cardiomyocytes was measured using the atomic force microscopy nanoindentation method in Tyrode buffer solution containing 1.8 mM Ca2+ and 5.4 mM KCl (physiological condition), 100 nM Ca2+ and 5.4 mM KCl (low extracellular Ca2+ condition), or 1.8 mM Ca2+ and 140 mM KCl (contraction condition). In the physiological condition, the mean AEM was 112% higher for live diabetic than control isolated cardiomyocytes (91 ± 14 vs. 43 ± 7 kPa). The AEM was also significantly higher in diabetic than control cardiomyocytes in the low extracellular Ca2+ and contraction conditions. These findings suggest that the material properties of live cardiomyocytes were affected by diabetes, resulting in stiffer cells, which very likely contribute to high diastolic LV stiffness, which has been observed in vivo in some diabetes mellitus patients.

Mapping of electrical double-layer force between tip and sample surfaces in water with pulsed-force-mode atomic force microscopy

1997 ◽  
Vol 71 (18) ◽  
pp. 2632-2634 ◽  
Author(s):  
Tatsuya Miyatani ◽  
Miki Horii ◽  
Armin Rosa ◽  
Masamichi Fujihira ◽  
Othmar Marti
Keyword(s):  
Atomic Force Microscopy ◽  
Double Layer ◽  
Electrical Double Layer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Force Mode ◽  
Mode Atomic Force Microscopy

Atomic force microscopy based analysis of cell-wall elasticity in macroalgae

2018 ◽  
pp. 335-347 ◽  
Author(s):  
Thomas Torode ◽  
Marina Linardic ◽  
J. Louis Kaplan ◽  
Siobhan A. Braybrook
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Force Microscopy ◽  
Cell Wall Elasticity ◽  
Atomic Force

scholarly journals In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy

Microscopy ◽  
2017 ◽  
Vol 66 (4) ◽  
pp. 272-282 ◽  
Author(s):  
Yanshu Zhang ◽  
Aiko Yoshida ◽  
Nobuaki Sakai ◽  
Yoshitsugu Uekusa ◽  
Masahiro Kumeta ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Actin Network ◽  
Cortical Actin ◽  
Force Microscopy ◽  
Atomic Force
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