Characterization of the complex carbohydrates in the zona pellucida of mammalian oocytes using lectin histochemistry

1996 ◽  
Vol 20 (3) ◽  
pp. 225-236 ◽  
Author(s):  
F. Parillo ◽  
G. Stradaioli ◽  
C. Dall'aglio ◽  
A. Verini-Supplizi
Keyword(s):  
Zona Pellucida ◽  
Lectin Histochemistry ◽  
Complex Carbohydrates

Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽  
2000 ◽  
pp. 325-335 ◽  
Author(s):  
A Calvo ◽  
LM Pastor ◽  
S Bonet ◽  
E Pinart ◽  
M Ventura
Keyword(s):  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Apical Part ◽  
Seminiferous Tubules ◽  
In Situ Characterization ◽  
Intense Staining ◽  
Terminal Galactose ◽  
Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽  
2000 ◽  
pp. 111-120 ◽  
Author(s):  
JA Chapman ◽  
OW Wiebkin ◽  
WG Breed
Keyword(s):  
Glycine Max ◽  
Sialic Acid ◽  
Zona Pellucida ◽  
Fluorescein Isothiocyanate ◽  
Interspecific Variation ◽  
Lectin Histochemistry ◽  
Sialic Acids ◽  
Arachis Hypogea ◽  
Marsupial Species ◽  
Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

scholarly journals Visco-Hyperelastic Characterization of the Equine Immature Zona Pellucida

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1223
Author(s):  
Elisa Ficarella ◽  
Mohammad Minooei ◽  
Lorenzo Santoro ◽  
Elisabetta Toma ◽  
Bartolomeo Trentadue ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Soft Matter ◽  
Mechanical Response ◽  
Mechanical Characterization ◽  
Nonlinear Material ◽  
Prony Series ◽  
Critical Comparison ◽  
Highly Nonlinear ◽  
Good Agreement

This article presents a very detailed study on the mechanical characterization of a highly nonlinear material, the immature equine zona pellucida (ZP) membrane. The ZP is modeled as a visco-hyperelastic soft matter. The Arruda–Boyce constitutive equation and the two-term Prony series are identified as the most suitable models for describing the hyperelastic and viscous components, respectively, of the ZP’s mechanical response. Material properties are identified via inverse analysis based on nonlinear optimization which fits nanoindentation curves recorded at different rates. The suitability of the proposed approach is fully demonstrated by the very good agreement between AFM data and numerically reconstructed force–indentation curves. A critical comparison of mechanical behavior of two immature ZP membranes (i.e., equine and porcine ZPs) is also carried out considering the information on the structure of these materials available from electron microscopy investigations documented in the literature.

scholarly journals Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

1989 ◽  
Vol 22 (1) ◽  
pp. 1-14 ◽  
Author(s):  
J. F. MADRID ◽  
J. BALLESTA ◽  
M. T. CASTELLS ◽  
J. A. MARIN ◽  
L. M. PASTOR
Keyword(s):  
Intestinal Mucosa ◽  
Lectin Histochemistry

Complex Carbohydrates of Red Wine: Characterization of the Extreme Diversity of Neutral Oligosaccharides by ESI-MS

2015 ◽  
Vol 63 (2) ◽  
pp. 671-682 ◽  
Author(s):  
Thierry Doco ◽  
Pascale Williams ◽  
Emmanuelle Meudec ◽  
Véronique Cheynier ◽  
Nicolas Sommerer
Keyword(s):  
Red Wine ◽  
Esi Ms ◽  
Complex Carbohydrates

Mechanical property characterization of mouse zona pellucida

2003 ◽  
Vol 2 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Yu Sun ◽  
Kai-Tak Wan ◽  
K.P. Roberts ◽  
J.C. Bischof ◽  
B.J. Nelson
Keyword(s):  
Mechanical Property ◽  
Zona Pellucida ◽  
Property Characterization

Lectin histochemistry of complex carbohydrates in human cervix

1985 ◽  
Vol 17 (6) ◽  
pp. 627-654 ◽  
Author(s):  
B. A. Schulte ◽  
K. C. Poon ◽  
K. P. P. Rao ◽  
S. S. Spicer
Keyword(s):  
Lectin Histochemistry ◽  
Complex Carbohydrates ◽  
Human Cervix

Characterization of Human Sperm Binding to Homologous Recombinant Zona Pellucida Proteins

2011 ◽  
Vol 18 (9) ◽  
pp. 876-885 ◽  
Author(s):  
Mayel Chirinos ◽  
Cecilia Cariño ◽  
María Elena González-González ◽  
Ernesto Arreola ◽  
Rodrigo Reveles ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Human Sperm ◽  
Sperm Binding
Sign in / Sign up

Export Citation Format

Share Document