scholarly journals Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

1989 ◽  
Vol 22 (1) ◽  
pp. 1-14 ◽  
Author(s):  
J. F. MADRID ◽  
J. BALLESTA ◽  
M. T. CASTELLS ◽  
J. A. MARIN ◽  
L. M. PASTOR
Keyword(s):  
Intestinal Mucosa ◽  
Lectin Histochemistry

Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽  
2000 ◽  
pp. 325-335 ◽  
Author(s):  
A Calvo ◽  
LM Pastor ◽  
S Bonet ◽  
E Pinart ◽  
M Ventura
Keyword(s):  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Apical Part ◽  
Seminiferous Tubules ◽  
In Situ Characterization ◽  
Intense Staining ◽  
Terminal Galactose ◽  
Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

Pathology of Pullorum Disease and Molecular Characterization of Its Pthogen

2020 ◽  
Vol 23 (1) ◽  
pp. 25-35
Author(s):  
MM Rahman ◽  
J Alam ◽  
MM Rahman ◽  
MAHNA Khan ◽  
MG Haider
Keyword(s):  
Molecular Characterization ◽  
Molecular Identification ◽  
Intestinal Mucosa ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Histopathological Study ◽  
Isolation And Identification ◽  
Focal Necrosis ◽  
Salmonella Pullorum

This experiment was conducted to determine the pathology of pullorum disease of chickens and molecular identification of its pathogen. A total of 108 samples, including swabs from different organs were collected from different commercial layer farms of Gazipur district. The histopathological samples were collected in 10% formalin and the swabs were collected in tetrathionate broth. Samples were subjected to isolation and identification of the causal agent followed by gross and histopathological study of the affected visceral organs. Fifty eight out of 108 cloacal swabs (53.7%) were Salmonella positive. The percentage of Salmonella positive in liver swabs, spleen swabs, lung swabs and intestinal swabs from dead birds were 55.88%, 32.35%, 35.29% and 47.05%, respectively. On average, 52.94% livers of Salmonella affected birds were enlarged, congested and hemorrhagic and necrotic foci was present in 32.35% liver. Unabsorbed and coagulated yolk was found in 70.58% cases. From these 38.24% spleens were swollen and congested and 44.12 % kidneys were enlarged. At histopathology, 52.94% livers showed congestion, focal necrosis with multifocal infiltration of histiocytes in liver parenchyma. Focal necrosis and inflammatory cells were found in 70.58% spleen. Infiltration of heterophils in intestinal mucosa was found in 47.05% cases. 20.58% (7 out of 34) samples were PCR positive for Salmonella Pullorum organism. Ann. Bangladesh Agric. (2019) 23(1) : 25-35

Identification and Characterization of Insulin Receptors in Basolateral Membranes of Dog Intestinal Mucosa

Diabetes ◽  
1987 ◽  
Vol 36 (10) ◽  
pp. 1124-1129 ◽  
Author(s):  
R. L. Gingerich ◽  
W. R. Gilbert ◽  
P. G. Comens ◽  
J. R. Gavin
Keyword(s):  
Intestinal Mucosa ◽  
Insulin Receptors ◽  
Basolateral Membranes ◽  
Identification And Characterization

scholarly journals Characterization of the sugar chain expression of normal term human placental villi using lectin histochemistry combined with immunohistochemistry

2009 ◽  
Vol 72 (1) ◽  
pp. 35-49 ◽  
Author(s):  
Ayano Tatsuzuki ◽  
Taichi Ezaki ◽  
Yasuo Makino ◽  
Yoshio Matsuda ◽  
Hiroaki Ohta
Keyword(s):  
Lectin Histochemistry ◽  
Sugar Chain ◽  
Normal Term ◽  
Placental Villi

scholarly journals Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae

2017 ◽  
Vol 61 (3) ◽  
Author(s):  
Gianluca Accogli ◽  
Giovanni Scillitani ◽  
Donatella Mentino ◽  
Salvatore Desantis
Keyword(s):  
Surface Layer ◽  
Lectin Histochemistry ◽  
The Body ◽  
Octopus Vulgaris ◽  
Mucous Cells ◽  
Cell Type ◽  
Skin Mucus ◽  
The Common ◽  
Common Octopus

<p>The O<em>ctopus vulgaris</em> farming is impaired by the high mortality of the paralarvae during the first month of life. Several factors have been investigated in this regard, but no data exist on the body surface mucus, which represents the interface with the outside environment. This study included morphometric analysis and glycoconjugates characterization of skin mucus in reared <em>Octopus vulgaris</em> paralarvae during the first month of life. Four types of mucous cells were distinguished:  mucous 1 (m1) and mucous 2 (m2) cells were scattered in the mantle epidermis, mucous 3 (m3) and mucous 4 (m4) in the epithelium surrounding the sucker. Except for the presence of fucosylated and neutral glycoconjugates in all mucous cells, each cell type expressed a characteristic glycopattern. m2 and m4 contained also suphate and acid non-sulphate glycans, m3 lacked suphate glycoproteins. Lectin histochemistry showed that mantle mucous cells (m1, m2) expressed GlcNAc and lactosamine terminating glycans. m2 also contained GalNAc terminal or penultimate to sialic acid. m3 was distinguished by mannosylated glycans terminating with lactosamine and m4 by α2,6 sialoglycans. Glycoproteins terminating with lactosamine, Galβ1,3GalNAc, and α1,6-linked fucose were a common feature of paralarvae surface layer. Morphometry revealed a significant decrease of m1 and m2 abundance during the first month of life, afterwards the reared paralarvae died. Since the glycopattern did not change during the investigated period, the mantle mucous cells abundance could be related to the <em>Octopus vulgaris</em> paralarvae survival. </p>

Heterotopic gastric mucosa of the rectum—Characterization of endocrine and mucin-producing cells by immunocytochemistry and lectin histochemistry

1989 ◽  
Vol 32 (2) ◽  
pp. 159-164 ◽  
Author(s):  
F. Carlei ◽  
R. Pietroletti ◽  
D. Lomanto ◽  
P. Barsotti ◽  
A. Crescenzi ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Lectin Histochemistry ◽  
Heterotopic Gastric Mucosa

scholarly journals Characterization of glycoconjugates in the bovine endometrium and chorion by lectin histochemistry

Reproduction ◽  
1989 ◽  
Vol 87 (2) ◽  
pp. 509-517 ◽  
Author(s):  
L. Munson ◽  
J. J. Kao ◽  
D. H. Schlafer
Keyword(s):  
Lectin Histochemistry
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