Lectin histochemistry of complex carbohydrates in human cervix

B. A. Schulte; K. C. Poon; K. P. P. Rao; S. S. Spicer

doi:10.1007/bf01003516

Characterization of the complex carbohydrates in the zona pellucida of mammalian oocytes using lectin histochemistry

Veterinary Research Communications ◽

10.1007/bf00366920 ◽

1996 ◽

Vol 20 (3) ◽

pp. 225-236 ◽

Cited By ~ 12

Author(s):

F. Parillo ◽

G. Stradaioli ◽

C. Dall'aglio ◽

A. Verini-Supplizi

Keyword(s):

Zona Pellucida ◽

Lectin Histochemistry ◽

Complex Carbohydrates

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Comparison of proteoglycan expression in rat and human cervix using lectin histochemistry

American Journal of Obstetrics and Gynecology ◽

10.1016/j.ajog.2005.10.125 ◽

2005 ◽

Vol 193 (6) ◽

pp. S44

Author(s):

Huiling Ji ◽

Michael House ◽

Vit Long ◽

Paul McMillan ◽

Edward Chien

Keyword(s):

Lectin Histochemistry ◽

Human Cervix

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An Ultrastructural Study of the Proliferation Patterns of the Reserve Cells of the Human Cervix Uteri

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100093651 ◽

1972 ◽

Vol 30 ◽

pp. 80-81

Author(s):

Takanori Sohda ◽

Hiroshi Saito ◽

Goro Asano ◽

Katsunari Fukushi ◽

Katsuya Suzuki ◽

...

Keyword(s):

Ultrastructural Study ◽

Age Groups ◽

Point Of View ◽

University Hospital ◽

Cervix Uteri ◽

Morphological Aspect ◽

Functional Aspect ◽

Pathological Conditions ◽

Human Cervix ◽

Morphological Patterns

Recently, the functional aspect as well as morphological aspect of the reserve cells in the cervix uteri drew much attention in view of the carcinogenesis in squamocolumunar junction. In this communication, the authors elucidate the ultrastructural features of the reserve cells in patients of various age groups visiting our university hospital and affiliated hospital.From conventional light microscopic point of view, the reserve cells tend to be pronounced in various pathological conditions, such as the persisting inflammation, proliferative disorders and irritation of hormones. The morphological patterns of the reserve cells from various stage and degree of irritation were observed.

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Complex Carbohydrates Part D

10.1016/s0076-6879(00)x0263-7 ◽

1982 ◽

Keyword(s):

Part D ◽

Complex Carbohydrates

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Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽

10.1530/reprod/120.2.325 ◽

2000 ◽

pp. 325-335 ◽

Cited By ~ 29

Author(s):

A Calvo ◽

LM Pastor ◽

S Bonet ◽

E Pinart ◽

M Ventura

Keyword(s):

Ricinus Communis ◽

Lectin Histochemistry ◽

Apical Part ◽

Seminiferous Tubules ◽

In Situ Characterization ◽

Intense Staining ◽

Terminal Galactose ◽

Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

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Interspecific variation of zona pellucida glycoconjugates in several species of marsupial

Reproduction ◽

10.1530/reprod/119.1.111 ◽

2000 ◽

pp. 111-120 ◽

Cited By ~ 5

Author(s):

JA Chapman ◽

OW Wiebkin ◽

WG Breed

Keyword(s):

Glycine Max ◽

Sialic Acid ◽

Zona Pellucida ◽

Fluorescein Isothiocyanate ◽

Interspecific Variation ◽

Lectin Histochemistry ◽

Sialic Acids ◽

Arachis Hypogea ◽

Marsupial Species ◽

Similar Intensity

The zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. Ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. Sections were also incubated with either neuraminidase or saponified, respectively, before incubation with the lectins to identify saccharide residues masked by sialic acids or O-acetyl groups on sialic acids. The zonae pellucidae surrounding the oocytes of the marsupials demonstrated interspecific variation in glycoconjugate content, with mannose-containing glycoconjugates exhibiting the greatest variation. Some of the zona pellucida glycoconjugates of all species, except those of the opossums, were masked by sialic acid with an increase in fluorescence with lectins from Arachis hypogea (PNA), and Glycine max (SBA), after desialylation. The disaccharide beta-galactose(1-4)N-acetyl-D-glucosamine appeared to be conformationally masked by O-acetyl groups of sialic acids in the zonae pellucidae of all species, with an increase in fluorescence with the lectin from Erythrina cristagalli (ECA), after saponification. Similar intensity and localization of beta-(1-4)-N-acetyl-D-glucosamine, as shown by staining of the lectin from Triticum vulgaris (WGA), to the inner and outer regions of the zona pellucida, were found to those reported in eutherian species. WGA fluorescence became uniform throughout the zonae pellucidae after saponification, indicating differential O-acetylation of sialic acids on the internal compartment of the zonae pellucidae.

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