Expression inEscherichia coli and immunological characterization of three zona pellucida proteins (ZP1, ZP2, and ZP3) from a marsupial, the brushtail possum (Trichosurus vulpecula)

2002 ◽  
Vol 64 (2) ◽  
pp. 136-143 ◽  
Author(s):  
Karen E. Mate ◽  
Janine M. Buist ◽  
Janine A. Duckworth
Keyword(s):  
Zona Pellucida ◽  
Brushtail Possum ◽  
Inescherichia Coli ◽  
Trichosurus Vulpecula ◽  
Immunological Characterization ◽  
Expression Inescherichia Coli

scholarly journals Immunogenicity and contraceptive potential of three infertility-relevant zona pellucida 2 epitopes in the marsupial brushtail possum (Trichosurus vulpecula)

Reproduction ◽  
2007 ◽  
Vol 133 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Janine A Duckworth ◽  
Kathryn Wilson ◽  
Xianlan Cui ◽  
Frank C Molinia ◽  
Phil E Cowan
Keyword(s):  
Amino Acids ◽  
Zona Pellucida ◽  
Keyhole Limpet Hemocyanin ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Incomplete Freund’S Adjuvant ◽  
Freund’S Adjuvant ◽  
Freund's Adjuvant ◽  
Phosphate Buffered Saline

In a previous study, three infertility-relevant epitopes of possum ZP2 (Pep12 (amino acids 111–125), Pep31 (amino acids 301–315), and Pep44 (amino acids 431–445)) were identified using sera from possums (Trichosurus vulpecula) immunized with recombinant possum zona pellucida 2 (ZP2) constructs, and a synthetic peptide library of possum ZP2 protein. In this study, the three peptides were conjugated to keyhole limpet hemocyanin and 300 μg of each conjugated peptide were administered subcutaneously to female possums (n = 20 per peptide) in complete Freund’s adjuvant. Immunogen doses were repeated 3 and 6 weeks later using incomplete Freund’s adjuvant. Control animals were immunized with either phosphate-buffered saline only (n = 10) or 300 μg keyhole limpet hemocyanin (n = 10), administered with the same adjuvants. Serum antibodies from animals immunized against these three epitopes bound to the corresponding possum ZP2 peptides, recombinant possum ZP2 protein constructs, and native zona. Possum fertility was assessed following superovulation and artificial insemination. Peptides Pep12 and Pep31 had no significant effects on fertility parameters (P > 0.05). However, animals immunized with Pep44 had lower egg fertilization rates (immunized 19.5% versus control 60.5%, P < 0.05) and produced significantly fewer embryos than control animals (immunized 0.5 embryos versus control 2.4 embryos, P < 0.05). The number of Pep44-immunized females that produced embryos was reduced by 64%. Identification and characterization of possum infertility-relevant epitopes on possum ZP2 protein will assist development of safe, humane, and possum-specific immunocontraceptive vaccines for controlling the introduced possums in New Zealand.

Chemical characterization of milk oligosaccharides of the common brushtail possum (Trichosurus vulpecula)

2014 ◽  
Vol 31 (5) ◽  
pp. 387-399 ◽  
Author(s):  
Tadasu Urashima ◽  
Saori Fujita ◽  
Kenji Fukuda ◽  
Tadashi Nakamura ◽  
Tadao Saito ◽  
...  
Keyword(s):  
Chemical Characterization ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Milk Oligosaccharides ◽  
The Common ◽  
Common Brushtail Possum

Cloning and characterization of a novel β-carotene hydroxylase gene fromLycium barbarumand its expression inEscherichia coli

2014 ◽  
Vol 61 (6) ◽  
pp. 637-645 ◽  
Author(s):  
Jiang Wu ◽  
Jing Ji ◽  
Gang Wang ◽  
Zhaodi Li ◽  
Jinjin Diao ◽  
...  
Keyword(s):  
Inescherichia Coli ◽  
Hydroxylase Gene ◽  
Expression Inescherichia Coli ◽  
Β Carotene

scholarly journals Erratum to: Chemical characterization of milk oligosaccharides of the common brushtail possum (Trichosurus vulpecula)

2014 ◽  
Vol 31 (8) ◽  
pp. 603-603
Author(s):  
Tadasu Urashima ◽  
Saori Fujita ◽  
Kenji Fukuda ◽  
Tadashi Nakamura ◽  
Tadao Saito ◽  
...  
Keyword(s):  
Chemical Characterization ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Milk Oligosaccharides ◽  
The Common ◽  
Common Brushtail Possum
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