The identification of human chromosomes by quinacrine fluorescence after hybridisation in situ

1980 ◽  
Vol 53 (3) ◽  
pp. 371-373 ◽  
Author(s):  
S. S. Lawrie ◽  
J. R. Gosden
Keyword(s):  
Human Chromosomes ◽  
Quinacrine Fluorescence

QUINACRINE FLUORESCENCE AND Q-BANDING PATTERNS OF HUMAN CHROMOSOMES.: I. Effects of Varying Factors

1975 ◽  
Vol 17 (1) ◽  
pp. 81-92 ◽  
Author(s):  
C. C. Lin ◽  
H. van de Sande ◽  
W. K. Smink ◽  
D. R. Newton
Keyword(s):  
Exposure Time ◽  
Uv Light ◽  
Human Chromosomes ◽  
Concave Mirror ◽  
Banding Patterns ◽  
Fluorescent Microscope ◽  
Sperm Dna ◽  
Quinacrine Fluorescence ◽  
Well Differentiated ◽  
Salmon Sperm Dna

Various factors involved in the production of "Q-bands" have been studied. It was found that a Zeiss standard WL fluorescent microscope required a shorter exposure time for photography as compared to a Zeiss photomicroscope. The minimal exposure time was obtained when the standard WL microscope was equipped with a UV light source containing a DC powered mercury burner and a concave mirror. Further, the pH and type of water used in the staining, washing and mounting of the slide were also important factors in producing clear and well differentiated "Q-bands". It also appears that the factors involved in the production of "Q-bands" effect the enhancement or quenching of fluorescence by poly d(A-T).poly d(A-T) and salmon sperm DNA or poly dG∙poly dC respectively. This preliminary report also suggests that DNA or polynucleotides with a specific base sequence may play an important role in Q-banding patterns on chromosomes.

Isolation of microcell hybrid clones containing retroviral vector insertions into specific human chromosomes

1987 ◽  
Vol 7 (8) ◽  
pp. 2814-2820
Author(s):  
T G Lugo ◽  
B Handelin ◽  
A M Killary ◽  
D E Housman ◽  
R E Fournier
Keyword(s):  
High Efficiency ◽  
Human Fibroblasts ◽  
Retroviral Vector ◽  
Human Chromosomes ◽  
G418 Resistance ◽  
Microcell Hybrid ◽  
Dominant Selectable Marker ◽  
Hybrid Clones ◽  
Microcell Fusion

We sought an efficient means to introduce specific human chromosomes into stable interspecific hybrid cells for applications in gene mapping and studies of gene regulation. A defective amphotropic retrovirus was used to insert the gene conferring G418 resistance (neo), a dominant selectable marker, into the chromosomes of diploid human fibroblasts, and the marked chromosomes were transferred to mouse recipient cells by microcell fusion. We recovered five microcell hybrid clones containing one or two intact human chromosomes which were identified by karyotype and marker analysis. Integration of the neo gene into a specific human chromosome in four hybrid clones was confirmed by segregation analysis or by in situ hybridization. We recovered four different human chromosomes into which the G418 resistance gene had integrated: human chromosomes 11, 14, 20, and 21. The high efficiency of retroviral vector transformation makes it possible to insert selectable markers into any mammalian chromosomes of interest.

Localisation of the classical DNA satellites on human chromosomes as determined by primed in situ labelling (PRINS)

1997 ◽  
Vol 100 (3-4) ◽  
pp. 322-326 ◽  
Author(s):  
A. J. Therkelsen ◽  
A. Nielsen ◽  
S. K�lvraa
Keyword(s):  
Human Chromosomes

Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase

1973 ◽  
Vol 18 (4) ◽  
pp. 297-306 ◽  
Author(s):  
D. Warburton ◽  
A. F. Naylor ◽  
F. E. Warburton
Keyword(s):  
Spatial Relations ◽  
Human Chromosomes ◽  
Quinacrine Fluorescence

Progress in the molecular cytogenetics of man

1988 ◽  
Vol 319 (1194) ◽  
pp. 239-248 ◽  
Keyword(s):  
Dna Sequences ◽  
Recombinant Dna ◽  
Molecular Cytogenetics ◽  
Chromosome Analysis ◽  
Parental Origin ◽  
Human Chromosomes ◽  
Recombinant Dna Technology ◽  
Chromosome Deletions ◽  
Dna Technology

Recombinant DNA technology has contributed greatly to the precision of chromosome analysis in man. Breakpoints of chromosome deletions and rearrangements may be defined on a chromosome map whose landmarks are the loci of DNA sequences rather than Giemsa bands. Flow cytogenetics allows the extent of chromosome duplications and deletions to be measured more precisely than has hitherto been possible. DNA probes can reveal hidden translocations through the application of in situ hybridization, and may be used as markers to determine the parental origin of non-disjunction. It is evident that a study of the pathology of human chromosomes now requires the combined skills of recombinant DNA and cytology.

scholarly journals Fluorescence In Situ Hybridization (FISH) on Human Chromosomes Using Photoprobe Biotin-labeled Probes

2003 ◽  
Vol 51 (4) ◽  
pp. 549-551 ◽  
Author(s):  
Anja Weise ◽  
Peter Harbarth ◽  
Uwe Claussen ◽  
Thomas Liehr
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Dna Probes ◽  
Human Chromosomes ◽  
First Time ◽  
Established Technique ◽  
Whole Chromosome Painting

Fluorescence in situ hybridization (FISH) on human chromosomes in meta-and interphase is a well-established technique in clinical and tumor cytogenetics and for studies of evolution and interphase architecture. Many different protocols for labeling the DNA probes used for FISH have been published. Here we describe for the first time the successful use of Photoprobe biotin-labeled DNA probes in FISH experiments. Yeast artificial chromosome (YAC) and whole chromosome painting (wcp) probes were tested.

An in Situ Study of Variant Telomeric Repeats in Human Chromosomes

Genomics ◽  
1999 ◽  
Vol 58 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Kateřina Krejčí ◽  
Jørn Koch
Keyword(s):  
Human Chromosomes ◽  
Telomeric Repeats ◽  
In Situ Study
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