Localisation of the classical DNA satellites on human chromosomes as determined by primed in situ labelling (PRINS)

1997 ◽  
Vol 100 (3-4) ◽  
pp. 322-326 ◽  
Author(s):  
A. J. Therkelsen ◽  
A. Nielsen ◽  
S. K�lvraa
Keyword(s):  
Human Chromosomes

Isolation of microcell hybrid clones containing retroviral vector insertions into specific human chromosomes

1987 ◽  
Vol 7 (8) ◽  
pp. 2814-2820
Author(s):  
T G Lugo ◽  
B Handelin ◽  
A M Killary ◽  
D E Housman ◽  
R E Fournier
Keyword(s):  
High Efficiency ◽  
Human Fibroblasts ◽  
Retroviral Vector ◽  
Human Chromosomes ◽  
G418 Resistance ◽  
Microcell Hybrid ◽  
Dominant Selectable Marker ◽  
Hybrid Clones ◽  
Microcell Fusion

We sought an efficient means to introduce specific human chromosomes into stable interspecific hybrid cells for applications in gene mapping and studies of gene regulation. A defective amphotropic retrovirus was used to insert the gene conferring G418 resistance (neo), a dominant selectable marker, into the chromosomes of diploid human fibroblasts, and the marked chromosomes were transferred to mouse recipient cells by microcell fusion. We recovered five microcell hybrid clones containing one or two intact human chromosomes which were identified by karyotype and marker analysis. Integration of the neo gene into a specific human chromosome in four hybrid clones was confirmed by segregation analysis or by in situ hybridization. We recovered four different human chromosomes into which the G418 resistance gene had integrated: human chromosomes 11, 14, 20, and 21. The high efficiency of retroviral vector transformation makes it possible to insert selectable markers into any mammalian chromosomes of interest.

Progress in the molecular cytogenetics of man

1988 ◽  
Vol 319 (1194) ◽  
pp. 239-248 ◽  
Keyword(s):  
Dna Sequences ◽  
Recombinant Dna ◽  
Molecular Cytogenetics ◽  
Chromosome Analysis ◽  
Parental Origin ◽  
Human Chromosomes ◽  
Recombinant Dna Technology ◽  
Chromosome Deletions ◽  
Dna Technology

Recombinant DNA technology has contributed greatly to the precision of chromosome analysis in man. Breakpoints of chromosome deletions and rearrangements may be defined on a chromosome map whose landmarks are the loci of DNA sequences rather than Giemsa bands. Flow cytogenetics allows the extent of chromosome duplications and deletions to be measured more precisely than has hitherto been possible. DNA probes can reveal hidden translocations through the application of in situ hybridization, and may be used as markers to determine the parental origin of non-disjunction. It is evident that a study of the pathology of human chromosomes now requires the combined skills of recombinant DNA and cytology.

scholarly journals Fluorescence In Situ Hybridization (FISH) on Human Chromosomes Using Photoprobe Biotin-labeled Probes

2003 ◽  
Vol 51 (4) ◽  
pp. 549-551 ◽  
Author(s):  
Anja Weise ◽  
Peter Harbarth ◽  
Uwe Claussen ◽  
Thomas Liehr
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Dna Probes ◽  
Human Chromosomes ◽  
First Time ◽  
Established Technique ◽  
Whole Chromosome Painting

Fluorescence in situ hybridization (FISH) on human chromosomes in meta-and interphase is a well-established technique in clinical and tumor cytogenetics and for studies of evolution and interphase architecture. Many different protocols for labeling the DNA probes used for FISH have been published. Here we describe for the first time the successful use of Photoprobe biotin-labeled DNA probes in FISH experiments. Yeast artificial chromosome (YAC) and whole chromosome painting (wcp) probes were tested.

An in Situ Study of Variant Telomeric Repeats in Human Chromosomes

Genomics ◽  
1999 ◽  
Vol 58 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Kateřina Krejčí ◽  
Jørn Koch
Keyword(s):  
Human Chromosomes ◽  
Telomeric Repeats ◽  
In Situ Study

A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP

2008 ◽  
Vol 28 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jörn Bullerdiek ◽  
Jürgen Dittmer ◽  
Angelika Faehre ◽  
Sabine Bartnitzke ◽  
Volker Kasche ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Human Chromosomes ◽  
Nick Translation ◽  
In Situ Nick Translation ◽  
Eco Ri

scholarly journals Differences in the Localization and Morphology of Chromosomes in the Human Nucleus

1999 ◽  
Vol 145 (6) ◽  
pp. 1119-1131 ◽  
Author(s):  
Jenny A. Croft ◽  
Joanna M. Bridger ◽  
Shelagh Boyle ◽  
Paul Perry ◽  
Peter Teague ◽  
...  
Keyword(s):  
Cell Cycle ◽  
In Situ Hybridization ◽  
Chromosome Morphology ◽  
Human Chromosomes ◽  
Chromosome 18 ◽  
Chromosome Architecture ◽  
Internal Position ◽  
Preferential Localization ◽  
Human Chromosome 19

Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19. Human chromosome 19 is shown to adopt a more internal position in the nucleus than chromosome 18 and to be more extensively associated with the nuclear matrix. The more peripheral localization of chromosome 18 is established early in the cell cycle and is maintained thereafter. We show that the preferential localization of chromosomes 18 and 19 in the nucleus is reflected in the orientation of translocation chromosomes in the nucleus. Lastly, we show that the inhibition of transcription can have gross, but reversible, effects on chromosome architecture. Our data demonstrate that the distribution of genomic sequences between chromosomes has implications for nuclear structure and we discuss our findings in relation to a model of the human nucleus that is functionally compartmentalized.

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