The differential banding pattern produced by Actinomycin-D/Acridine-Orange counterstaining in metaphase chromosomes ofDrosophila melanogaster

1981 ◽  
Vol 37 (8) ◽  
pp. 822-823 ◽  
Author(s):  
R. Mezzanotte ◽  
L. Ferrucci
Keyword(s):  
Acridine Orange ◽  
Banding Pattern ◽  
Actinomycin D ◽  
Metaphase Chromosomes

scholarly journals Different sensitivity of chromatin to acid denaturation in quiescent and cycling cells as revealed by flow cytometry.

1979 ◽  
Vol 27 (1) ◽  
pp. 478-485 ◽  
Author(s):  
Z Darzynkiewicz ◽  
F Traganos ◽  
M Andreeff ◽  
T Sharpless ◽  
M R Melamed
Keyword(s):  
Acridine Orange ◽  
Condensed Chromatin ◽  
Metaphase Chromosomes ◽  
Quiescent Cells ◽  
Acid Denaturation ◽  
Interphase Cells ◽  
The Difference ◽  
Cell Subpopulations ◽  
G1 Cells ◽  
Dna Complex

The properties of DNA in situ as reflected by its staining with acridine orange are different in quiescent nonstimulated lymphocytes as compared with interphase lymphocytes that have entered the cell cycle after stimulation by mitogens. The difference is seen after cell treatment with buffers at pH 1.5 (1.3-1.9 range) followed by staining with acridine orange at pH 2.6 (2.3-2.9). Under these conditions the red metachromatic fluorescence of the acridine orange-DNA complex is higher in quiescent cells than in the cycling lymphocytes while the orthochromatic green fluorescence is higher in the cycling, interphase cells. The results suggest that DNA in condensed chromatin of quiescent lymphocytes (as in metaphase chromosomes) is more sensitive to acid-denaturation than DNA in dispersed chromatin of the cycling interphase cells. The phenomenon is used for flow cytometric differentiation between G0 and G1 cells and between G2 and M cells. In contrast to normal lymphocytes the method applied to neoplastic cells indicates the presence of cell subpopulations with condensed chromatin but with DNA content characteristic not only of G1 but also of S and G2 cells. The possibility that these cells represent quiescent (resting) subpopulations, arrested in G1, S and/or G2, is discussed.

Acridine Orange Potentiation of Actinomycin D Uptake and Activity

Science ◽  
1971 ◽  
Vol 174 (4010) ◽  
pp. 696-698 ◽  
Author(s):  
E. F. Roth ◽  
J. Kochen
Keyword(s):  
Acridine Orange ◽  
Actinomycin D

C-banded karyotypes of Brassica campestris, B. oleracea, and B. napus

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 583-589 ◽  
Author(s):  
Majlis Olin-Fatih ◽  
W. K. Heneen
Keyword(s):  
Banding Pattern ◽  
Chromosome Pair ◽  
Brassica Campestris ◽  
Brassica Species ◽  
Conclusive Evidence ◽  
Metaphase Chromosomes ◽  
Late Prophase ◽  
Nucleolar Chromosome ◽  
Centromeric Position ◽  
Similar Banding Pattern

The chromosome complements of three Brassica species, namely B. campestris (2n = 20), B. oleracea (2n = 18), and B. napus (2n = 38), were studied using the air-dry method and C-banding. Karyotypes and ideograms of late prophase chromosomes were constructed, since contracted metaphase chromosomes were generally not suitable for this purpose. The three species generally had a similar banding pattern, manifested in the presence of a centromeric C-band in all chromosomes and heterochromatic knobs at the telomeric end of some chromosomes. The centromeric C-bands were more pronounced in B. campestris than in B. oleracea. Depending on the centromeric position, the chromosomes were grouped into median, submedian, subterminal, and terminal types. All chromosome pairs were morphologically distinguishable. Only one nucleolar chromosome pair, with heterochromatic satellites, was observed in each species. When compared, it was possible to distinguish chromosomes of both B. campestris and B. oleracea type in B. napus, but conclusive evidence as to the origin of all chromosome pairs in B. napus was not at hand.Key words: Brassica, chromosomes, late prophase, C-bands, knob structures, karyotypes, idiograms.

Banding in mitotic chromosomes of Brassica campestris var. pekinensis with a trypsin–Giemsa method

Genome ◽  
1992 ◽  
Vol 35 (5) ◽  
pp. 899-901 ◽  
Author(s):  
Soryu Nishibayasahi
Keyword(s):  
Banding Pattern ◽  
Brassica Campestris ◽  
Chromosome Size ◽  
Mitotic Chromosomes ◽  
Metaphase Chromosomes ◽  
Secondary Constrictions

In Brassica campestris var. pekinensis cv. CR-strong, the karyotype comprised 12 median, 6 submedian, and 2 sub-terminal chromosomes. Secondary constrictions were observed in the two subterminal chromosomes. Banding pattern appeared very clearly in metaphase chromosomes with a trypsin–Giemsa method. It was possible to classify the chromosomes into 10 types (C1–C10), based on the chromosome size, shape, and banding pattern.Key words: Brassica campestris var. pekinensis, mitotic chromosomes, G-banding.

scholarly journals Use of actinomycin D for the specific quenching of fluorescence of deoxyribonucleic acid in cells stained with acridine aminoderivatives.

1976 ◽  
Vol 24 (11) ◽  
pp. 1169-1172 ◽  
Author(s):  
A V Zelenin ◽  
E A Kirianova ◽  
V A Kolesnikov ◽  
N G Stepanova
Keyword(s):  
Acridine Orange ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Actinomycin D ◽  
Quenching Of Fluorescence ◽  
Approximate Estimation ◽  
In Cells

Actinomycin D specifically quenches the fluorescence of acridine orange and quinacrine bound to deoxyribonucleic acid in cytologic preparations, but does not change the fluorescence of these fluorochromes bound to RNA. The following fluorescence-cytochemical applications of techniques based on these findings can be suggested: (a) distinction between deoxyribonucleic acid and ribonucleic acid; (b) detection of double-stranded virus ribonucleic acid; (c) approximate estimation of the lengths of A-T sequences in deoxyribonucleic acid molecules.

scholarly journals Effect of actinomycin-D on the staining of whole cells with acridine orange.

1981 ◽  
Vol 29 (5) ◽  
pp. 644-648 ◽  
Author(s):  
S H Chambers ◽  
J V Watson
Keyword(s):  
Acridine Orange ◽  
Fluorescence Intensity ◽  
Actinomycin D ◽  
Whole Cells

Flow cytofluorometric techniques have been used to investigate the interference of actinomycin-D with the staining of acridine orange in whole cells. The results show that reduction in fluorescence intensity will only occur at actinomycin-D concentrations greater than 10(3) ng/ml in our system, providing the drug is washed out prior to staining.

Differential Acridine Orange Staining of Human Chromosomes

1972 ◽  
Vol 21 (4) ◽  
pp. 319-326
Author(s):  
G.L. Castoldi ◽  
G.D. Grusovin ◽  
G.L. Scapoli ◽  
R. Spanedda
Keyword(s):  
Acridine Orange ◽  
Banding Pattern ◽  
Human Chromosomes ◽  
Salt Solutions ◽  
Single Stranded Dna ◽  
Acridine Orange Staining ◽  
Double Stranded Dna ◽  
Previously Treated

SummaryThe acridine orange staining of metaphases previously treated with hot salt solutions, exhibits a differential banding pattern of the chromosomes. According to the physicochemical interpretation of the stained structures, the green and red fluorescent segments of the chromosomes should be considered as constituted respectively by double-stranded DNA and single-stranded DNA. The banding pattern is relatively consistent in different metaphases, although some occasional variations of the bands may be referred to the interference of chromosomal acid proteins. In general, the sequence of the bands is compatible with the picture of the reverse banding.

scholarly journals EVIDENCE FOR VARIATION IN THE QUANTITY OF DNA AMONG PLASTIDS OF ACETABULARIA

1970 ◽  
Vol 44 (2) ◽  
pp. 361-375 ◽  
Author(s):  
Christopher L. F. Woodcock ◽  
Lawrence Bogorad
Keyword(s):  
Electron Microscopy ◽  
Acridine Orange ◽  
Dna Content ◽  
Actinomycin D ◽  
Acridine Orange Staining ◽  
Tissue Sections ◽  
Unicellular Algae ◽  
Acetabularia Mediterranea

The DNA content of individual plastids of the giant unicellular algae Acetabularia mediterranea, and Polyphysa cliftoni was studied. Four methods were used for localizing DNA: acridine orange staining, radioautography following actinomycin D-3H treatment, electron microscopy of thin tissue sections, and electron microscopy of osomotically disrupted plastids. With each method, DNA was readily detected in 20–35% of plastids, but no DNA was observed in the remaining 65–80%. The results further showed that in those plastids with detectable DNA the amount of DNA present was variable. The sensitivity and reliability of the localization methods are discussed, and the possible implications of these findings are considered.

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