scholarly journals EVIDENCE FOR VARIATION IN THE QUANTITY OF DNA AMONG PLASTIDS OF ACETABULARIA

1970 ◽  
Vol 44 (2) ◽  
pp. 361-375 ◽  
Author(s):  
Christopher L. F. Woodcock ◽  
Lawrence Bogorad
Keyword(s):  
Electron Microscopy ◽  
Acridine Orange ◽  
Dna Content ◽  
Actinomycin D ◽  
Acridine Orange Staining ◽  
Tissue Sections ◽  
Unicellular Algae ◽  
Acetabularia Mediterranea

The DNA content of individual plastids of the giant unicellular algae Acetabularia mediterranea, and Polyphysa cliftoni was studied. Four methods were used for localizing DNA: acridine orange staining, radioautography following actinomycin D-3H treatment, electron microscopy of thin tissue sections, and electron microscopy of osomotically disrupted plastids. With each method, DNA was readily detected in 20–35% of plastids, but no DNA was observed in the remaining 65–80%. The results further showed that in those plastids with detectable DNA the amount of DNA present was variable. The sensitivity and reliability of the localization methods are discussed, and the possible implications of these findings are considered.

Microbial Biofilms in the Gut: Visualization by Electron Microscopy and by Acridine Orange Staining

2004 ◽  
Vol 28 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Daniel Palestrant ◽  
Zoie E. Holzknecht ◽  
Bradley H. Collins ◽  
William Parker ◽  
Sara E. Miller ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Acridine Orange ◽  
Acridine Orange Staining ◽  
Microbial Biofilms

Microbial Biofilms in the Gut: Visualization by Electron Microscopy and by Acridine Orange Staining

2004 ◽  
Vol 28 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Daniel Palestrant ◽  
Zoie E. Holzknecht ◽  
Bradley H. Collins ◽  
William Parker ◽  
Sara E. Miller ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Acridine Orange ◽  
Acridine Orange Staining ◽  
Microbial Biofilms

Head Size and DNA Content in Bacteriophage T4

1972 ◽  
Vol 30 ◽  
pp. 200-201
Author(s):  
Fred Eiserling ◽  
A. H. Doermann ◽  
Linde Boehner
Keyword(s):  
Electron Microscopy ◽  
Dna Content ◽  
Bacteriophage T4 ◽  
Head Size ◽  
Virus Particles ◽  
Altered Protein ◽  
A Cell ◽  
Bacterial Viruses ◽  
A Site ◽  
Protein Shell

The control of form or shape inheritance can be approached by studying the morphogenesis of bacterial viruses. Shape variants of bacteriophage T4 with altered protein shell (capsid) size and nucleic acid (DNA) content have been found by electron microscopy, and a mutant (E920g in gene 66) controlling head size has been described. This mutant produces short-headed particles which contain 2/3 the normal DNA content and which are non-viable when only one particle infects a cell (Fig. 1).We report here the isolation of a new mutant (191c) which also appears to be in gene 66 but at a site distinct from E920g. The most striking phenotype of the mutant is the production of about 10% of the phage yield as “giant” virus particles, from 3 to 8 times longer than normal phage (Fig. 2).

STUDIES ON THE BIOLOGICAL PROPERTIES AND CLASSIFICATION OF SV4 VIRUS

1965 ◽  
Vol 11 (2) ◽  
pp. 325-335 ◽  
Author(s):  
S. A. Sattar ◽  
K. R. Rozee
Keyword(s):  
Electron Microscope ◽  
Rhesus Monkey ◽  
Red Blood Cells ◽  
Acridine Orange ◽  
Blood Cells ◽  
Magnesium Chloride ◽  
Fluorescent Microscopy ◽  
Biological Properties ◽  
Acridine Orange Staining

Cytopathic changes in LLC-MK2 cells infected with SV4 virus, observed with the electron microscope and using acridine orange staining and fluorescent microscopy, have been shown to be similar to that caused by picornaviruses and members of the Columbia-SK virus group. The virus was found to be stabilized against heat in the presence of molar magnesium chloride, and to be stable at pH 3.5. The virus was non-pathogenic for suckling mice, failed to agglutinate sheep and human "O" red blood cells, but agglutinated rhesus monkey erythrocytes at 4 °C. On the basis of these properties and those already known, it was suggested that SV4 virus be placed in the group Enteroviruses of lower animals.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 535-540 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Acridine Orange ◽  
Particle Number ◽  
Amoeba Proteus ◽  
Acridine Orange Staining ◽  
Particle Population ◽  
Cell Age ◽  
Cytoplasmic Dna ◽  
Number Of Particles

Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.

Molecular Hybridization of Mouse Satellite DNA-Complementary RNA in Ultrathin Sections Prepared for Electron Microscopy

1974 ◽  
Vol 14 (2) ◽  
pp. 253-261
Author(s):  
J. JACOB ◽  
KATHERINE GILLIES ◽  
D. MACLEOD ◽  
K. W. JONES
Keyword(s):  
Electron Microscopy ◽  
Satellite Dna ◽  
Similar Distribution ◽  
Tissue Sections ◽  
Satellite Sequences ◽  
Interphase Cells ◽  
Nucleolar Chromatin ◽  
Ultrathin Sections ◽  
Mouse Satellite Dna

The feasibility of in situ hybridization in tissue sections prepared for electron microscopy has been examined using mouse satellite DNA-complementary RNA and mouse L cells. The results obtained are encouraging, although certain technical aspects require further clarification. In interphase cells, hybrid-forming sites occur in chromatin patches positioned along the nuclear envelope. It is also confirmed that satellite DNA occurs in nucleolus-associated chromatin. The results suggest that satellite sequences are present in intranucleolar and peri-nucleolar chromatin. A similar distribution is indicated for ribosomal cistrons.

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