Comparison of different pollen viability assays to evaluate pollen fertility of potato dihaploids

Euphytica ◽  
1991 ◽  
Vol 56 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Bodo R. Trognitz
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability

scholarly journals Biology of Flowering, Fruit and Viability of Pollen of Varieties and Forms of Quince Cultivated on the Territory of the Nakhchivan Autonomous Republic

2021 ◽  
Vol 7 (3) ◽  
pp. 64-69
Author(s):  
L. Bayramov
Keyword(s):  
Pollen Fertility ◽  
Pollen Germination ◽  
Glucose Solution ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Weather Conditions ◽  
Distilled Water ◽  
Water Control ◽  
Germinated Pollen ◽  
Autonomous Republic

Abstract. The zones of distribution of varieties and forms of quince on the territory of the Nakhchivan Autonomous Republic have been established, phenological observations have been carried out, their flowering and fruiting have been studied. On the territory of the Autonomous Republic, flowering of varieties and forms of quince begins in the second decade of April, depending on the distribution zone, with an average daily temperature of 12–13 °C and lasts 12–13 days, depending on weather conditions. Each flower has 10–12 stamens arranged in one row. The article also studied the viability of pollen in a number of quince varieties. Pollen viability was studied in the varieties Sary, Tursh, Ordubad, Gara and wild forms. Pollen fertility was determined by staining with acetocarmine. Pollen germinates in 2–5–10–15 and 20% glucose solution. Counting of germinated pollen grains was carried out under a microscope. The study showed that of all the experimental varieties, the pollen fertility of the Sary quince and Tursh quince varieties is high (up to 96.6–97.1%). The best medium for the germination of quince pollen is a 10–15% glucose solution. Pollen germination in this solution reaches 47.4–88.0%. In distilled water (control), the germination of quince pollen reached from 9.7% to 35.6% for varieties. Quince pollen remains viable for 31–43 days.

scholarly journals The influence of pollen viability on seed set and fruit mass in strawberry (fragaria x ananassa duch.)

2012 ◽  
Vol 61 (1) ◽  
pp. 79-84
Author(s):  
Elżbieta Kaczmarska ◽  
Agnieszka M. Dobrowolska ◽  
Jerzy A. Hortyński
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Great Influence ◽  
Pollen Grains ◽  
Fragaria X Ananassa ◽  
Strawberry Fruit ◽  
Viable Pollen ◽  
Small Fruit ◽  
Horticultural Plant ◽  
Fruit Mass

This research was conducted in 2006 in the Experimental Station of the Department of Genetics and Horticultural Plant Breeding in Felin near Lublin. It included 7 cultivars of strawberry ('Pastel', 'Salut', 'Teresa', 'Jota', 'Paula', 'Senga Sengana', 'Plena') and two selected clones: 2395 and 3995 that belonged to the Department. Analysis of pollen fertility was carried out on the basis of materials collected on five occasions: on May 17 (at the beginning of florescence), May 20 and May 24 (the peak of florescence) and on June 2 and June 13 (the end of florescence). Smear preparations stained with 2% acetocarmin and glycerin solution (1:1) were used to test pollen viability. The highest percent of viable pollen in 2006 was in cultivar 'Jota' (70.88%). The lowest viability had pollen of 'Teresa' cultivar (33.83%). The average content of viable pollen grains was the lowest on May 20 (36.79%). The highest pollen fertility was noted on June 2 (62.15%), the percent of fertile pollen on a similar level was observed on May 17 and 24 and June13. The cultivar 'Jota' was characterized by an increased level of pollen fertility that was quite high during the whole period of florescence. The number of seeds has a great influence on the proper development of spurious strawberry fruit. The mass of seeds from a single fruit has a great influence on the mass of fruits in both large and small fruit categories. Pollen viability affected the mass of seeds on big fruits (r = 0.444), but there is no clear direct relationship between pollen fertility and mass of strawberry fruit(r = -0.193 and r = -0.052).

scholarly journals Pollen Fertility Status in Coastal Plant Species of Rotuma Island

2002 ◽  
Vol 20 (1) ◽  
pp. 30 ◽  
Author(s):  
Reijieli R. Rigamoto ◽  
Anand P. Tyagi
Keyword(s):  
Population Size ◽  
Plant Species ◽  
Pollen Fertility ◽  
Pollen Viability ◽  
Coastal Areas ◽  
High Pollen ◽  
Fertility Status ◽  
Coastal Species ◽  
Wide Range ◽  
Coastal Plant

and Abstract The Island of Rotuma is facing widespread destruction and degradation of its flora. This investigation was conducted to determine the pollen fertility status in thirty two species of economic and environmentally important coastal species, which are reproducing freely thus most suitable to rehabilitate coastal areas. Pollen fertility status in these species varied from as low as 38% in Canavalia cathertica to as high as 100% in Hernandia nymphaeifolia and Vigna marina. It was interesting to note that species within same genus showed wide range of pollen viability. However, most of the species investigated had high pollen viability especially in those species, which grow close to sea. It was recorded that pollen fertility was low in small fragmented populations. This showed the effect of population size on reproduction. Larger sized populations showed high percentage of pollen fertility.

scholarly journals Molecular characterization of two natural hybrids from the Iberian Peninsula: Ranunculus × luizetii and R. × peredae (Ranunculaceae)

Biologia ◽  
2012 ◽  
Vol 67 (5) ◽  
Author(s):  
Eduardo Cires ◽  
Candela Cuesta ◽  
José Prieto
Keyword(s):  
Pollen Fertility ◽  
Fragment Length Polymorphism ◽  
Pollen Viability ◽  
Mean Value ◽  
Aflp Markers ◽  
Rdna Sequences ◽  
The Mean ◽  
Rdna Polymorphisms ◽  
Fertility Estimation

AbstractTwo nothospecies, Ranunculus × luizetii and R. × peredae (Ranunculaceae), were analyzed and discussed. For this purpose, Amplified Fragment Length Polymorphism (AFLP) markers, nuclear rDNA sequences (ITS1, 5.8S and ITS2) and pollen viability were conducted. The profiles of these hybrid samples were compared to their putative progenitors. Several additive polymorphic sites detected in the ITS sequences of the hybrid samples (R. × luizetii and R. × peredae) also confirmed their derived origins from ribotypes of their parental taxa (R. parnassiifolius subsp. parnassiifolius × R. pyrenaeus; R. amplexicaulis × R. cabrerensis subsp. cabrerensis, respectively). Despite the lack of exclusive AFLP markers reported in both hybrids, presumably due to effects of introgression, the concerted evolution of many rDNA polymorphisms towards either of the parental ribotypes indicated their ancient origin. Pollen fertility estimation in R. × luizetii presented a mean value of 60.58%, which showed that hybrid samples are well established and fertile. However, a larger difference was observed in R. × peredae, where the mean value of pollen fertility was very low (18.91%).

Pollen fertility in Musa: Viability in cultivars grown in Southern Australia

2004 ◽  
Vol 55 (10) ◽  
pp. 1085 ◽  
Author(s):  
J. A. Fortescue ◽  
D. W. Turner
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Diploid Species ◽  
Female Fertility ◽  
Viable Pollen ◽  
Meiotic Restitution ◽  
Different Levels

Pollen viability was examined by recording the number of viable as well as non-viable microspores in anthers just before anthesis, as it could be an efficient and rapid indicator of female fertility. It was thought that competency in meiotic restitution in microspores could indicate similar competency in megaspores. Pollen fertility was compared among seeded diploids and edible triploids of Musa and correlated with ovule fertility. Viability was examined using the Alexander’s pollen stain procedure. The seeded diploid species M. acuminata, M. balbisiana, and M. ornata had 3 times more viable pollen than the edible tetraploids (AAAB). M. balbisiana and M. ornata had significantly more viable pollen than M. acuminata. The tetraploids contained 3 times more viable pollen than the edible triploids AAA and ABB and 4 times more than the AAB cultivars. The genome A or B did not affect pollen viability within the triploid cultivars examined. The AAA triploid Gros Michel had the highest percentage of viable pollen at 13% and Green/Red the lowest at 3%. Pollen viability was influenced by meiotic disturbances and an association was made between viability of pollen and viability of embryo sacs. Pollen viability from anthers of Australian grown species and cultivars of Musa a. ssp. (undescribed subspecies) was compared with published reports of pollen viability from Indian grown species and cultivars. The pollen viability between the Indian cultivated and wild diploids of Musa a. ssp. was similar at 50–66%, but less than the pollen viability of Australian diploid Musa a. ssp of 84%. The Indian-grown triploid cultivars had 21–29% viable pollen and the tetraploids had 28% viable pollen, whereas the Australian grown triploids had 6–10% viable pollen and the tetraploids 29% viable pollen. There was a difference in pollen viability between genome groups and within genome groups. Different species and cultivars of Musa possess different levels of competency in the production of microspores, which correlated positively with levels of megaspore fertility in the same species and cultivars.

scholarly journals No Correlation Between Pollen Fertility and Viability: Differential Measures of Male Gametophytic Fitness in Cannabis sativa L.

2021 ◽  
Author(s):  
Sydney B Wizenberg ◽  
Michelle Dang ◽  
Lesley Geills Campbell
Keyword(s):  
Pollen Fertility ◽  
Storage Time ◽  
Cannabis Sativa ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Relative Fitness ◽  
Male Fitness ◽  
Single Measure ◽  
Viability Analysis ◽  
Population Evolution

Pollen grains are male gametophytes, an ephemeral haploid generation of plants, commonly engaging in competition for a limited supply of ovules. Since differential male fertility may influence the direction and pace of population evolution, the relative fitness of pollen is regularly estimated as either pollen viability, the proportion of pollen containing intact cytoplasm's and regenerative nuclei, or pollen fertility, the frequency of pollen germinating under standardized conditions. Here, we estimated the relative fitness of pollen in a dioecious, wind-pollinated model system, Cannabis sativa, by characterizing pollen fertility and viability from multiple sires. Pollen fertility quickly declined within two weeks of anther dehiscence, and pollen stored under freezer conditions did not germinate regardless of storage time. In contrast, pollen viability declined slowly and persisted longer than the lifetime of a sporophyte plant under both room temperature and freezer conditions. Pollen samples that underwent both fertility and viability analysis displayed no significant correlation, implying researchers cannot predict pollen fertility from pollen viability, nor infer male gametophytic fitness from a single measure. Our work demonstrates two approaches to measure proxies of male fitness in C. sativa, and identifies new questions around what are valuable estimates of male fitness in plants.

scholarly journals SCREENING CAPSICUM GENOTYPES FOR INCREASED DROUGHT TOLERANCE BY IN VITRO POLLEN GERMINATION AND POLLEN TUBE LENGTH

2020 ◽  
Vol 23 (1) ◽  
pp. 52-58
Author(s):  
Y. Arnaoudova ◽  
B. Arnaoudov
Keyword(s):  
Drought Tolerance ◽  
Pollen Fertility ◽  
Pollen Germination ◽  
Male Gametophyte ◽  
Pollen Viability ◽  
Pollen Tubes ◽  
Tube Length ◽  
Vegetable Crops ◽  
Lower Productivity

Yield realizing in vegetable crops depends on several reproductive processes that include pollen fertility as well as pollen tubes germination and growth. The study was conducted as the pollen characteristics fertility and viability were used, as well as the productivity for evaluation of drought tolerance in four pepper genotypes Capsicum annuum L. From the conducted cytological analyses was found that decreasing the water standard with 50% exert negative influence over the male gametophyte and the pollen fertility decreases with up to 37.4% average. More sensitive to drought showed to be the plants from both cultivars Pazardzhishka kapia and Albena, in which lower productivity and lower restorable potential were found in comparison with those found in the mutant lines No.1936 and No.1917. Increased tolerance showed the male gametophyte of line No.1936. After a drought in it were found the highest values for pollen viability – 33.4% average pollen germination and 438.7µm pollen tubes length which was with proven mathematical difference compared with the rest of the genotypes. These results showed that pollen fertility analyses and pollen viability analyses in drought plants could be used for evaluation of the drought tolerance.

scholarly journals The effects of fosetyl-Al application on morphology and viability of Lycopersicon esculentum Mill. pollen

2008 ◽  
Vol 54 (No. 8) ◽  
pp. 336-340 ◽  
Author(s):  
İ. Öztürk Çalı
Keyword(s):  
Lycopersicon Esculentum ◽  
Pollen Fertility ◽  
Pollen Viability ◽  
Polar View ◽  
The Other ◽  
Control Group ◽  
Recommended Dosage ◽  
Viable Pollen ◽  
Pollen Types ◽  
Viability Level

In the present study, the effects of fungicide Aliette WG 800 [80% fosetyl-Al (aluminium tris-o-ethyl phosphonate)], widely used against <I>Phytophtora infestans</I> on tomatoes grown in greenhouse in Turkey, were studied on the morphology and viability of tomato (<I>Lycopersicon esculentum</I> Mill.) pollens. The fungicide was applied to tomatoes grown in greenhouse at recommended dosage (200 g/100 l water) and at double the recommended dosage (400 g/100 l water). The fungicide caused changes in the morphological structures of tomato pollens. Some pollen morphological structures that are not observed in the control group were encountered in the pollens in equatorial view and in polar view at 200 g/100 l treated groups. On the other hand, pollen viability level decreased as the dosage increased. Especially, non-viable pollen types such as wrinkled pollen or pollen with abnormal shape were encountered in the fungicide groups. It was expected that the pollen fertility as well as yield would decrease in future.

Pollen Biology of Calligonum mongolicum in the Middle Reaches of Heihe River

2011 ◽  
Vol 356-360 ◽  
pp. 2917-2920
Author(s):  
Cheng Chen Pan ◽  
Lin De Liu ◽  
Ha Lin Zhao ◽  
Xue Yong Zhao ◽  
Yue Li Hou ◽  
...  
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Fertility Rate ◽  
Heihe River ◽  
Pollen Production ◽  
Significant Difference ◽  
Pollen Longevity ◽  
Single Flower ◽  
Pollen Biology

The pollen–ovule ratios, pollen fertility rate, pollen viability and longevity, pollen histochemistry of Calligonum mongolicum flowers were determined in the field by the middle reaches of Heihe River. The main results were as follows: Calligonum mongolicum is completely fertile and has starchy pollen. Anthers matured heterotypically. The pollen longevity was about 10h. The pollen viability was higher than 70% at the beginning of anthesis on sunny days. No difference in the pollen production of single anther was found between different flower morphes, but significant difference was in that of single flower. The pollen-ovule ratios of different morph flowers were 1625±125, 1750±177, 1500±102, 1563±120, 1875±72. Pollen–ovule ratios (P/O) reveal that C. mongolicum is facultative autogamous.

Pollen Biology of Robinia pseudoacacia on Zhen Mountain in Yantai

2011 ◽  
Vol 356-360 ◽  
pp. 2748-2751
Author(s):  
Cheng Chen Pan ◽  
Lin De Liu ◽  
Ha Lin Zhao ◽  
Xue Yong Zhao ◽  
Li Zhang ◽  
...  
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Robinia Pseudoacacia ◽  
Pollen Grains ◽  
Fertility Rate ◽  
Shandong Province ◽  
Pollen Longevity ◽  
Pollen Biology

The pollen–ovule ratios, pollen fertility rate, pollen viability and longevity, pollen histochemistry of Robinia pseudoacacia flowers were determined in the field on Zhen Mountain in Yantai, Shandong province in China. The main results were as follows: Robinia pseudoacacia is completely fertile and has starchy pollen. The pollen longevity was about 80h. The pollen viability was higher than 90% at the beginning of anthesis on sunny days. 80h later, most pollen grains lose their viability. Pollen–ovule ratio (P/O) is 192±19. Accoring to Cruden, R. pseudoacacia is facultative autogamous.

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