Pollen fertility in Musa: Viability in cultivars grown in Southern Australia

2004 ◽  
Vol 55 (10) ◽  
pp. 1085 ◽  
Author(s):  
J. A. Fortescue ◽  
D. W. Turner
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Diploid Species ◽  
Female Fertility ◽  
Viable Pollen ◽  
Meiotic Restitution ◽  
Different Levels

Pollen viability was examined by recording the number of viable as well as non-viable microspores in anthers just before anthesis, as it could be an efficient and rapid indicator of female fertility. It was thought that competency in meiotic restitution in microspores could indicate similar competency in megaspores. Pollen fertility was compared among seeded diploids and edible triploids of Musa and correlated with ovule fertility. Viability was examined using the Alexander’s pollen stain procedure. The seeded diploid species M. acuminata, M. balbisiana, and M. ornata had 3 times more viable pollen than the edible tetraploids (AAAB). M. balbisiana and M. ornata had significantly more viable pollen than M. acuminata. The tetraploids contained 3 times more viable pollen than the edible triploids AAA and ABB and 4 times more than the AAB cultivars. The genome A or B did not affect pollen viability within the triploid cultivars examined. The AAA triploid Gros Michel had the highest percentage of viable pollen at 13% and Green/Red the lowest at 3%. Pollen viability was influenced by meiotic disturbances and an association was made between viability of pollen and viability of embryo sacs. Pollen viability from anthers of Australian grown species and cultivars of Musa a. ssp. (undescribed subspecies) was compared with published reports of pollen viability from Indian grown species and cultivars. The pollen viability between the Indian cultivated and wild diploids of Musa a. ssp. was similar at 50–66%, but less than the pollen viability of Australian diploid Musa a. ssp of 84%. The Indian-grown triploid cultivars had 21–29% viable pollen and the tetraploids had 28% viable pollen, whereas the Australian grown triploids had 6–10% viable pollen and the tetraploids 29% viable pollen. There was a difference in pollen viability between genome groups and within genome groups. Different species and cultivars of Musa possess different levels of competency in the production of microspores, which correlated positively with levels of megaspore fertility in the same species and cultivars.

scholarly journals The influence of pollen viability on seed set and fruit mass in strawberry (fragaria x ananassa duch.)

2012 ◽  
Vol 61 (1) ◽  
pp. 79-84
Author(s):  
Elżbieta Kaczmarska ◽  
Agnieszka M. Dobrowolska ◽  
Jerzy A. Hortyński
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Great Influence ◽  
Pollen Grains ◽  
Fragaria X Ananassa ◽  
Strawberry Fruit ◽  
Viable Pollen ◽  
Small Fruit ◽  
Horticultural Plant ◽  
Fruit Mass

This research was conducted in 2006 in the Experimental Station of the Department of Genetics and Horticultural Plant Breeding in Felin near Lublin. It included 7 cultivars of strawberry ('Pastel', 'Salut', 'Teresa', 'Jota', 'Paula', 'Senga Sengana', 'Plena') and two selected clones: 2395 and 3995 that belonged to the Department. Analysis of pollen fertility was carried out on the basis of materials collected on five occasions: on May 17 (at the beginning of florescence), May 20 and May 24 (the peak of florescence) and on June 2 and June 13 (the end of florescence). Smear preparations stained with 2% acetocarmin and glycerin solution (1:1) were used to test pollen viability. The highest percent of viable pollen in 2006 was in cultivar 'Jota' (70.88%). The lowest viability had pollen of 'Teresa' cultivar (33.83%). The average content of viable pollen grains was the lowest on May 20 (36.79%). The highest pollen fertility was noted on June 2 (62.15%), the percent of fertile pollen on a similar level was observed on May 17 and 24 and June13. The cultivar 'Jota' was characterized by an increased level of pollen fertility that was quite high during the whole period of florescence. The number of seeds has a great influence on the proper development of spurious strawberry fruit. The mass of seeds from a single fruit has a great influence on the mass of fruits in both large and small fruit categories. Pollen viability affected the mass of seeds on big fruits (r = 0.444), but there is no clear direct relationship between pollen fertility and mass of strawberry fruit(r = -0.193 and r = -0.052).

scholarly journals The effects of fosetyl-Al application on morphology and viability of Lycopersicon esculentum Mill. pollen

2008 ◽  
Vol 54 (No. 8) ◽  
pp. 336-340 ◽  
Author(s):  
İ. Öztürk Çalı
Keyword(s):  
Lycopersicon Esculentum ◽  
Pollen Fertility ◽  
Pollen Viability ◽  
Polar View ◽  
The Other ◽  
Control Group ◽  
Recommended Dosage ◽  
Viable Pollen ◽  
Pollen Types ◽  
Viability Level

In the present study, the effects of fungicide Aliette WG 800 [80% fosetyl-Al (aluminium tris-o-ethyl phosphonate)], widely used against <I>Phytophtora infestans</I> on tomatoes grown in greenhouse in Turkey, were studied on the morphology and viability of tomato (<I>Lycopersicon esculentum</I> Mill.) pollens. The fungicide was applied to tomatoes grown in greenhouse at recommended dosage (200 g/100 l water) and at double the recommended dosage (400 g/100 l water). The fungicide caused changes in the morphological structures of tomato pollens. Some pollen morphological structures that are not observed in the control group were encountered in the pollens in equatorial view and in polar view at 200 g/100 l treated groups. On the other hand, pollen viability level decreased as the dosage increased. Especially, non-viable pollen types such as wrinkled pollen or pollen with abnormal shape were encountered in the fungicide groups. It was expected that the pollen fertility as well as yield would decrease in future.

scholarly journals Biology of Flowering, Fruit and Viability of Pollen of Varieties and Forms of Quince Cultivated on the Territory of the Nakhchivan Autonomous Republic

2021 ◽  
Vol 7 (3) ◽  
pp. 64-69
Author(s):  
L. Bayramov
Keyword(s):  
Pollen Fertility ◽  
Pollen Germination ◽  
Glucose Solution ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Weather Conditions ◽  
Distilled Water ◽  
Water Control ◽  
Germinated Pollen ◽  
Autonomous Republic

Abstract. The zones of distribution of varieties and forms of quince on the territory of the Nakhchivan Autonomous Republic have been established, phenological observations have been carried out, their flowering and fruiting have been studied. On the territory of the Autonomous Republic, flowering of varieties and forms of quince begins in the second decade of April, depending on the distribution zone, with an average daily temperature of 12–13 °C and lasts 12–13 days, depending on weather conditions. Each flower has 10–12 stamens arranged in one row. The article also studied the viability of pollen in a number of quince varieties. Pollen viability was studied in the varieties Sary, Tursh, Ordubad, Gara and wild forms. Pollen fertility was determined by staining with acetocarmine. Pollen germinates in 2–5–10–15 and 20% glucose solution. Counting of germinated pollen grains was carried out under a microscope. The study showed that of all the experimental varieties, the pollen fertility of the Sary quince and Tursh quince varieties is high (up to 96.6–97.1%). The best medium for the germination of quince pollen is a 10–15% glucose solution. Pollen germination in this solution reaches 47.4–88.0%. In distilled water (control), the germination of quince pollen reached from 9.7% to 35.6% for varieties. Quince pollen remains viable for 31–43 days.

scholarly journals Reproductive characterization of interspecific hybrids among Capsicum species

2011 ◽  
Vol 11 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Carlos Eduardo da Silva Monteiro ◽  
Telma Nair Santana Pereira ◽  
Karina Pereira de Campos
Keyword(s):  
Male Sterility ◽  
Interspecific Hybrids ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Male Sterile ◽  
Viable Pollen ◽  
Hybrid Combination ◽  
Capsicum Species ◽  
The Cross

The objective of this study was the reproductive characterization of Capsicum accessions as well as of interspecific hybrids, based on pollen viability. Hybrids were obtained between Capsicum species. Pollen viability was high in most accessions, indicating that meiosis is normal, resulting in viable pollen grains. The pollen viability of species C. pubescens was the lowest (27 %). The interspecific hybrids had varying degrees of pollen viability, from fertile combinations (C. chinense x C. frutescens and C. annuum x C. baccatum) to male sterile combinations. Pollen viability also varied within the hybrid combination according to accessions used in the cross. Results indicate that male sterility is one of the incompatibility barriers among Capsicum species since hybrids can be established, but may be male sterile.

scholarly journals Salt-inducible Kinases Are Critical Determinants of Female Fertility

Endocrinology ◽  
2020 ◽  
Vol 161 (7) ◽  
Author(s):  
Marah Armouti ◽  
Nicola Winston ◽  
Osamu Hatano ◽  
Elie Hobeika ◽  
Jennifer Hirshfeld-Cytron ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Follicle Stimulating Hormone ◽  
Female Fertility ◽  
Camp Signaling ◽  
Follicle Development ◽  
Pharmacological Modulation ◽  
Ovarian Granulosa Cells ◽  
Different Levels

Abstract Follicle development is the most crucial step toward female fertility and is controlled mainly by follicle-stimulating hormone (FSH). In ovarian granulosa cells (GCs), FSH activates protein kinase A by increasing 3′,5′-cyclic adenosine 5′-monophosphate (cAMP). Since cAMP signaling is impinged in part by salt-inducible kinases (SIKs), we examined the role of SIKs on the regulation of FSH actions. Here, we report that SIKs are essential for normal ovarian function and female fertility. All SIK isoforms are expressed in human and rodent GCs at different levels (SIK3&gt;SIK2&gt;SIK1). Pharmacological inhibition of SIK activity potentiated the stimulatory effect of FSH on markers of GC differentiation in mouse, rat, and human GCs and estradiol production in rat GCs. In humans, SIK inhibition strongly enhanced FSH actions in GCs of patients with normal or abnormal ovarian function. The knockdown of SIK2, but not SIK1 or SIK3, synergized with FSH on the induction of markers of GC differentiation. SIK inhibition boosted gonadotropin-induced GC differentiation in vivo, while the genomic knockout of SIK2 led to a significant increase in the number of ovulated oocytes. Conversely, SIK3 knockout females were infertile, FSH insensitive, and had abnormal folliculogenesis. These findings reveal novel roles for SIKs in the regulation of GC differentiation and female fertility, and contribute to our understanding of the mechanisms regulated by FSH. Furthermore, these data suggest that specific pharmacological modulation of SIK2 activity could be of benefit to treat ovulatory defects in humans and to increase the propagation of endangered species and farm mammals.

Study on the homology of the genomes of tetraploid Asiatic lilies (Lilium) using FISH

Genome ◽  
2015 ◽  
Vol 58 (11) ◽  
pp. 453-461 ◽  
Author(s):  
Shujun Zhou ◽  
Lei Zhong ◽  
Lu Zhang ◽  
Zhenghua Xu ◽  
Xuxin Liu ◽  
...  
Keyword(s):  
Chromosome Doubling ◽  
Pollen Viability ◽  
Diploid Species ◽  
45S Rdna ◽  
Chromosomal Association

Asiatic lily cultivars, bred by hybridization and (or) chromosome doubling of species of section Sinomartagon of Lilium, are diploid, triploid, or tetraploid, but the homology of the genomes among species of section Sinomartagon and Asiatic lilies remains unclear. In the present research, two tetraploid Asiatic cultivars were analyzed, using 45S rDNA as probe, for their FISH karyotypes and their chromosomal association, anaphase I, telophase II, and pollen viability were surveyed to assess the multivalent segregation. Chromosomal assortment of six progenies of the two tetraploid cultivars were also investigated. The results showed that the tetraploid cultivars had similar FISH karyotypes, they predominantly formed multivalents, and these were equally separated because their anaphase I, telophase II, and pollen viability were similar to those of diploid species. Apart from minor variations, FISH karyotypes of progenies were similar to each other and to their parents. Based on these results and considering the high crossability among species of section Sinomartagon and (or) Asiatic lilies, we concluded that species of section Sinomartagon and their resulting cultivars share a common genome; thus, polyploidy Asiatic lilies are autopolyploid.

scholarly journals Characterization and Field Performance of 15 Strawberry Germplasm under Bangladesh Conditions

2014 ◽  
Vol 11 (2) ◽  
pp. 81-94 ◽  
Author(s):  
M Moshiur Rahman ◽  
M Mizanur Rahman ◽  
M Mofazzal Hossain ◽  
MA Khaleque Mian ◽  
Q Abdul Khaliq
Keyword(s):  
Plant Height ◽  
Fruit Set ◽  
Pollen Viability ◽  
Field Performance ◽  
Viable Pollen ◽  
Days To Flowering ◽  
Qualitative And Quantitative ◽  
Quantitative Characters ◽  
Number Of Leaves

Fifteen strawberry germplasm collected from local and exotic sources were evaluated for different qualitative and quantitative characters. The germplasm showed variation for majority of the studied traits. Plant height during peak harvest ranged from 14.67 to 25.00 cm and FA 007 exhibited the tallest plants. The maximum number of leaves plant-1 was produced by FA 006 (46.67). The maximum number of runners plant-1 was obtained in FA 003 (68.67) followed by FA 004 (63.00), while the highest number of crown plant-1 was found in FA 007 (15.33) followed by FA 006 (14.67). Days to flowering varied from 48.33 to 102.30 among the germplasm and FA 008 required minimum (48.33) days for flowering. The germplasm FA 004 produced the highest number of flower trusses (35.50 plant-1) while it was the lowest in FA 010 (5.00 plant-1). The number of flowers plant-1 was found maximum in FA 003 (168.00 plant-1) and minimum in FA 010 (40.00 plant-1). Among the germplasm pollen viability varied significantly and maximum viable pollen was recorded in FA 010 (84 %), while it was lowest in FA 004 (12.00 %). The highest per cent fruit set was recorded in BARI Strawberry-1 (86 %) while, FA 013 (39 %) showed the lowest. Among the germplasm the highest yield plant-1 was recorded from FA 005 (737.70 g) followed by FA 006 (702.30 g) and was significantly higher than others while the lowest yield plant-1 was recorded from FA 013 (52.00 g), FA 014 (69.00 g), FA 009 (81.33 g) and FA 010 (121.30 g). DOI: http://dx.doi.org/10.3329/sja.v11i2.18404 SAARC J. Agri., 11(2): 81-94 (2013)

scholarly journals Pollen grain traits of oil species from the Novi Sad collection

Genetika ◽  
2009 ◽  
Vol 41 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Jovanka Atlagic ◽  
Ana Marjanovic-Jeromela ◽  
Radovan Marinkovic ◽  
Sreten Terzic
Keyword(s):  
Staining Method ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Viable Pollen ◽  
Continuous Work ◽  
Novi Sad ◽  
Pollen Grain Morphology ◽  
Specific Number

The collection of oil species in Novi Sad contains 12 species represented with 1-4 cultivars or landraces. In the continuous work on this collection in the sense of breeding of some of those species and their usage as a source of 'desirable genes' we analyzed pollen grain morphology (shape and size), as well as pollen viability. To determine mentioned pollen traits we used Axiovert 40C microscope together with a software package (AxioVision LE; Rel.4.3.) for measurement of pollen length and width. Pollen viability was determined using a staining method (ALEXANDER, 1969). The results showed that species differ by pollen grain shape (round, egg-shaped, triangular and rod) as well as by shape of exine (thick and spiky, thick to thin). In some species there was a specific number of apertures present (1-11). The size of viable pollen grains ranged from 29,10/12,58? (coriander) to 176,63/169,94? (oil gourd), while non-viable pollen grains were always smaller (27,27/10,97? to 119,62/100,86?) at the same plant species. Pollen viability of most species was around 80%. Lowest pollen viability was found in white flax (56,98%), and the highest in oil pumpkin (91,43%).

scholarly journals Pollen Viability of Selected Diploid Watermelon Pollenizer Cultivars

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 274-275 ◽  
Author(s):  
Josh H. Freeman ◽  
Stephen M. Olson ◽  
Eileen A. Kabelka
Keyword(s):  
Fruit Quality ◽  
Fruit Set ◽  
Citrullus Lanatus ◽  
Pollen Viability ◽  
Viable Pollen ◽  
Diploid Cultivar ◽  
Triploid Watermelon

In the Spring and Fall 2006, the pollen viability of four diploid watermelon pollenizers was evaluated in Quincy, FL. Triploid watermelon plants [Citrullus lanatus (Thunb.) Matsum. & Nakai.] do not produce sufficient viable pollen to pollenize themselves and a diploid cultivar must be interplanted as a pollen source. Recent studies have illustrated differences in triploid watermelon yields as a result of the pollenizer cultivar used. The viability of the pollen produced by pollenizer cultivars may greatly influence the fruit set and fruit quality in the triploid watermelon crop. Pollen samples were taken from ‘Companion’, ‘Jenny’, ‘Mickylee’, and ‘SP-1’ and were stained to determine their viability. There were no significant differences in pollen viability among cultivars and all cultivars had high average viability. Pollen viability was never lower than 95% for any cultivar. This study indicates that pollen viability of the cultivars evaluated should not influence their effectiveness as pollenizers.

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