scholarly journals The influence of pollen viability on seed set and fruit mass in strawberry (fragaria x ananassa duch.)

2012 ◽  
Vol 61 (1) ◽  
pp. 79-84
Author(s):  
Elżbieta Kaczmarska ◽  
Agnieszka M. Dobrowolska ◽  
Jerzy A. Hortyński
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Great Influence ◽  
Pollen Grains ◽  
Fragaria X Ananassa ◽  
Strawberry Fruit ◽  
Viable Pollen ◽  
Small Fruit ◽  
Horticultural Plant ◽  
Fruit Mass

This research was conducted in 2006 in the Experimental Station of the Department of Genetics and Horticultural Plant Breeding in Felin near Lublin. It included 7 cultivars of strawberry ('Pastel', 'Salut', 'Teresa', 'Jota', 'Paula', 'Senga Sengana', 'Plena') and two selected clones: 2395 and 3995 that belonged to the Department. Analysis of pollen fertility was carried out on the basis of materials collected on five occasions: on May 17 (at the beginning of florescence), May 20 and May 24 (the peak of florescence) and on June 2 and June 13 (the end of florescence). Smear preparations stained with 2% acetocarmin and glycerin solution (1:1) were used to test pollen viability. The highest percent of viable pollen in 2006 was in cultivar 'Jota' (70.88%). The lowest viability had pollen of 'Teresa' cultivar (33.83%). The average content of viable pollen grains was the lowest on May 20 (36.79%). The highest pollen fertility was noted on June 2 (62.15%), the percent of fertile pollen on a similar level was observed on May 17 and 24 and June13. The cultivar 'Jota' was characterized by an increased level of pollen fertility that was quite high during the whole period of florescence. The number of seeds has a great influence on the proper development of spurious strawberry fruit. The mass of seeds from a single fruit has a great influence on the mass of fruits in both large and small fruit categories. Pollen viability affected the mass of seeds on big fruits (r = 0.444), but there is no clear direct relationship between pollen fertility and mass of strawberry fruit(r = -0.193 and r = -0.052).

scholarly journals Biology of Flowering, Fruit and Viability of Pollen of Varieties and Forms of Quince Cultivated on the Territory of the Nakhchivan Autonomous Republic

2021 ◽  
Vol 7 (3) ◽  
pp. 64-69
Author(s):  
L. Bayramov
Keyword(s):  
Pollen Fertility ◽  
Pollen Germination ◽  
Glucose Solution ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Weather Conditions ◽  
Distilled Water ◽  
Water Control ◽  
Germinated Pollen ◽  
Autonomous Republic

Abstract. The zones of distribution of varieties and forms of quince on the territory of the Nakhchivan Autonomous Republic have been established, phenological observations have been carried out, their flowering and fruiting have been studied. On the territory of the Autonomous Republic, flowering of varieties and forms of quince begins in the second decade of April, depending on the distribution zone, with an average daily temperature of 12–13 °C and lasts 12–13 days, depending on weather conditions. Each flower has 10–12 stamens arranged in one row. The article also studied the viability of pollen in a number of quince varieties. Pollen viability was studied in the varieties Sary, Tursh, Ordubad, Gara and wild forms. Pollen fertility was determined by staining with acetocarmine. Pollen germinates in 2–5–10–15 and 20% glucose solution. Counting of germinated pollen grains was carried out under a microscope. The study showed that of all the experimental varieties, the pollen fertility of the Sary quince and Tursh quince varieties is high (up to 96.6–97.1%). The best medium for the germination of quince pollen is a 10–15% glucose solution. Pollen germination in this solution reaches 47.4–88.0%. In distilled water (control), the germination of quince pollen reached from 9.7% to 35.6% for varieties. Quince pollen remains viable for 31–43 days.

scholarly journals Reproductive characterization of interspecific hybrids among Capsicum species

2011 ◽  
Vol 11 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Carlos Eduardo da Silva Monteiro ◽  
Telma Nair Santana Pereira ◽  
Karina Pereira de Campos
Keyword(s):  
Male Sterility ◽  
Interspecific Hybrids ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Male Sterile ◽  
Viable Pollen ◽  
Hybrid Combination ◽  
Capsicum Species ◽  
The Cross

The objective of this study was the reproductive characterization of Capsicum accessions as well as of interspecific hybrids, based on pollen viability. Hybrids were obtained between Capsicum species. Pollen viability was high in most accessions, indicating that meiosis is normal, resulting in viable pollen grains. The pollen viability of species C. pubescens was the lowest (27 %). The interspecific hybrids had varying degrees of pollen viability, from fertile combinations (C. chinense x C. frutescens and C. annuum x C. baccatum) to male sterile combinations. Pollen viability also varied within the hybrid combination according to accessions used in the cross. Results indicate that male sterility is one of the incompatibility barriers among Capsicum species since hybrids can be established, but may be male sterile.

scholarly journals Pollen grain traits of oil species from the Novi Sad collection

Genetika ◽  
2009 ◽  
Vol 41 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Jovanka Atlagic ◽  
Ana Marjanovic-Jeromela ◽  
Radovan Marinkovic ◽  
Sreten Terzic
Keyword(s):  
Staining Method ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Viable Pollen ◽  
Continuous Work ◽  
Novi Sad ◽  
Pollen Grain Morphology ◽  
Specific Number

The collection of oil species in Novi Sad contains 12 species represented with 1-4 cultivars or landraces. In the continuous work on this collection in the sense of breeding of some of those species and their usage as a source of 'desirable genes' we analyzed pollen grain morphology (shape and size), as well as pollen viability. To determine mentioned pollen traits we used Axiovert 40C microscope together with a software package (AxioVision LE; Rel.4.3.) for measurement of pollen length and width. Pollen viability was determined using a staining method (ALEXANDER, 1969). The results showed that species differ by pollen grain shape (round, egg-shaped, triangular and rod) as well as by shape of exine (thick and spiky, thick to thin). In some species there was a specific number of apertures present (1-11). The size of viable pollen grains ranged from 29,10/12,58? (coriander) to 176,63/169,94? (oil gourd), while non-viable pollen grains were always smaller (27,27/10,97? to 119,62/100,86?) at the same plant species. Pollen viability of most species was around 80%. Lowest pollen viability was found in white flax (56,98%), and the highest in oil pumpkin (91,43%).

scholarly journals Pollen Grain and Hybridization Studies in the Genus Capsicum

2016 ◽  
Vol 8 (1) ◽  
pp. 134-138 ◽  
Author(s):  
Tomi Lois OLATUNJI ◽  
Joseph Akintade MORAKINYO
Keyword(s):  
Success Rate ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Interspecific Crosses ◽  
Putative Hybrid ◽  
Gene Exchange ◽  
Viable Pollen ◽  
Pollination Success ◽  
Capsicum Species

The current study aimed to evaluate the pollen viability of the commonly cultivated varieties of Capsicum species and assessed the potentials for gene exchange among the genotypes through hybridization studies. Capsicum annuum var. abbreviatum, C. annuum var. acuminatum, C. annuum var. grossum and C. frutescens var. baccatum were the species and varieties used in this study. The present findings indicated that the percentage of pollen viability varied in the studied Capsicum genotypes. The highest pollen viability was obtained in C. annuum var. abbreviatum (96.3%), followed by C. annuum var. grossum (95%), and C. annuum var. acuminatum (91.1%). The lowest pollen viability was recorded in C. frutescens var. baccatum (86.2%). The pollen viability was high in most varieties indicating that meiosis is normal, resulting in viable pollen grains. Several intraspecific and interspecific crosses were performed among the Capsicum genotypes and three putative hybrid fruits were produced. Percentage successes obtained in the crosses were low and comparable in both intra and inter-specific crosses. In the entire crosses pattern, pollination success of 10% was recorded for C. frutescens var. baccatum and C. annuum var. acuminatum. Knowing the nature and viability of pollen grains may help in predicting the success rate of hybridization and the successful crosses between C. frutescens var. baccatum and C. annuum var. acuminatum suggest that these two varieties are the closest genetically. 

scholarly journals In vitro pollen viability of maize cultivars at different times of collection

2017 ◽  
Vol 47 (2) ◽  
Author(s):  
Emanoel Sanches Martins ◽  
Livia Maria Chamma Davide ◽  
Gian José Miranda ◽  
Jefferson de Oliveira Barizon ◽  
Francisco de Assis Souza Junior ◽  
...  
Keyword(s):  
Pollen Viability ◽  
Linear Regression Analysis ◽  
Pollen Grains ◽  
Viable Pollen ◽  
Average Percentage ◽  
Maize Cultivars ◽  
Solid Media ◽  
Tetrazolium Staining ◽  
Clustering Test

ABSTRACT: In the present study, we aimed to assess the in vitro viability of pollen grains from maize cultivars collected at different times and days in the field. Four cultivars (Sol da Manhã, XB 6012, XB 8010, and BRS 2020) were evaluated from the second to fifth day of anthesis in three times. Pollen samples were evaluated for their in vitro viability through standard germination test in liquid and solid media and tetrazolium staining. The experimental design was completely randomized, in 4x4x3 split-split plots with four replicates. Data were subjected to analysis of variance followed by a means clustering test and linear regression analysis. The average percentage of viable pollen varied according to the day, collection time, and cultivar. In general, XB 8010 and BRS 2020 had the highest frequency of viable pollen. The highest percentages of viable pollen were observed on the second day of anthesis at 10:00h.

scholarly journals Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

2004 ◽  
Vol 47 (5) ◽  
pp. 703-712 ◽  
Author(s):  
Milena Barcelos Cardoso ◽  
Eliane Kaltchuk-Santos ◽  
Elsa Cristina de Mundstock ◽  
Maria Helena Bodanese-Zanettini
Keyword(s):  
Chromosome Doubling ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Chromosome Counts ◽  
Viable Pollen ◽  
Stage Of Development ◽  
Initial Segmentation ◽  
Bud Size

Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).

Pollen fertility in Musa: Viability in cultivars grown in Southern Australia

2004 ◽  
Vol 55 (10) ◽  
pp. 1085 ◽  
Author(s):  
J. A. Fortescue ◽  
D. W. Turner
Keyword(s):  
Pollen Fertility ◽  
Pollen Viability ◽  
Diploid Species ◽  
Female Fertility ◽  
Viable Pollen ◽  
Meiotic Restitution ◽  
Different Levels

Pollen viability was examined by recording the number of viable as well as non-viable microspores in anthers just before anthesis, as it could be an efficient and rapid indicator of female fertility. It was thought that competency in meiotic restitution in microspores could indicate similar competency in megaspores. Pollen fertility was compared among seeded diploids and edible triploids of Musa and correlated with ovule fertility. Viability was examined using the Alexander’s pollen stain procedure. The seeded diploid species M. acuminata, M. balbisiana, and M. ornata had 3 times more viable pollen than the edible tetraploids (AAAB). M. balbisiana and M. ornata had significantly more viable pollen than M. acuminata. The tetraploids contained 3 times more viable pollen than the edible triploids AAA and ABB and 4 times more than the AAB cultivars. The genome A or B did not affect pollen viability within the triploid cultivars examined. The AAA triploid Gros Michel had the highest percentage of viable pollen at 13% and Green/Red the lowest at 3%. Pollen viability was influenced by meiotic disturbances and an association was made between viability of pollen and viability of embryo sacs. Pollen viability from anthers of Australian grown species and cultivars of Musa a. ssp. (undescribed subspecies) was compared with published reports of pollen viability from Indian grown species and cultivars. The pollen viability between the Indian cultivated and wild diploids of Musa a. ssp. was similar at 50–66%, but less than the pollen viability of Australian diploid Musa a. ssp of 84%. The Indian-grown triploid cultivars had 21–29% viable pollen and the tetraploids had 28% viable pollen, whereas the Australian grown triploids had 6–10% viable pollen and the tetraploids 29% viable pollen. There was a difference in pollen viability between genome groups and within genome groups. Different species and cultivars of Musa possess different levels of competency in the production of microspores, which correlated positively with levels of megaspore fertility in the same species and cultivars.

scholarly journals No Correlation Between Pollen Fertility and Viability: Differential Measures of Male Gametophytic Fitness in Cannabis sativa L.

2021 ◽  
Author(s):  
Sydney B Wizenberg ◽  
Michelle Dang ◽  
Lesley Geills Campbell
Keyword(s):  
Pollen Fertility ◽  
Storage Time ◽  
Cannabis Sativa ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Relative Fitness ◽  
Male Fitness ◽  
Single Measure ◽  
Viability Analysis ◽  
Population Evolution

Pollen grains are male gametophytes, an ephemeral haploid generation of plants, commonly engaging in competition for a limited supply of ovules. Since differential male fertility may influence the direction and pace of population evolution, the relative fitness of pollen is regularly estimated as either pollen viability, the proportion of pollen containing intact cytoplasm's and regenerative nuclei, or pollen fertility, the frequency of pollen germinating under standardized conditions. Here, we estimated the relative fitness of pollen in a dioecious, wind-pollinated model system, Cannabis sativa, by characterizing pollen fertility and viability from multiple sires. Pollen fertility quickly declined within two weeks of anther dehiscence, and pollen stored under freezer conditions did not germinate regardless of storage time. In contrast, pollen viability declined slowly and persisted longer than the lifetime of a sporophyte plant under both room temperature and freezer conditions. Pollen samples that underwent both fertility and viability analysis displayed no significant correlation, implying researchers cannot predict pollen fertility from pollen viability, nor infer male gametophytic fitness from a single measure. Our work demonstrates two approaches to measure proxies of male fitness in C. sativa, and identifies new questions around what are valuable estimates of male fitness in plants.

scholarly journals The effects of fosetyl-Al application on morphology and viability of Lycopersicon esculentum Mill. pollen

2008 ◽  
Vol 54 (No. 8) ◽  
pp. 336-340 ◽  
Author(s):  
İ. Öztürk Çalı
Keyword(s):  
Lycopersicon Esculentum ◽  
Pollen Fertility ◽  
Pollen Viability ◽  
Polar View ◽  
The Other ◽  
Control Group ◽  
Recommended Dosage ◽  
Viable Pollen ◽  
Pollen Types ◽  
Viability Level

In the present study, the effects of fungicide Aliette WG 800 [80% fosetyl-Al (aluminium tris-o-ethyl phosphonate)], widely used against <I>Phytophtora infestans</I> on tomatoes grown in greenhouse in Turkey, were studied on the morphology and viability of tomato (<I>Lycopersicon esculentum</I> Mill.) pollens. The fungicide was applied to tomatoes grown in greenhouse at recommended dosage (200 g/100 l water) and at double the recommended dosage (400 g/100 l water). The fungicide caused changes in the morphological structures of tomato pollens. Some pollen morphological structures that are not observed in the control group were encountered in the pollens in equatorial view and in polar view at 200 g/100 l treated groups. On the other hand, pollen viability level decreased as the dosage increased. Especially, non-viable pollen types such as wrinkled pollen or pollen with abnormal shape were encountered in the fungicide groups. It was expected that the pollen fertility as well as yield would decrease in future.

Development of the Anther and Ovule in Capsicum L

1978 ◽  
Vol 26 (3) ◽  
pp. 433 ◽  
Author(s):  
P Dharamadhaj ◽  
N Prakash
Keyword(s):  
Pollen Fertility ◽  
Embryo Sac ◽  
Pollen Grains ◽  
The Other ◽  
Flower Buds ◽  
Viable Pollen ◽  
Male And Female

The structure of the anther and ovule and the development of the male and female gametophytes have been studied in seven cultivated varieties of Capsicum-C. annuum L. var. acuminatum Fingerh. (long red cayenne), C. annuum L. cv. Floral Gem, C. annuum L, var. grossum (L.) Sendt. (giant bell), C. annuum var. longum (DC.) Sendt. (long sweet yellow), C. baccatum L. var. pendulum (Willd.) Eshbaugh, C. frutescens L, var. baccatum (L.) Irish and C. frutescens L, cv. Tabasco. Marked differences in the development were observed between flower buds formed in summer and in winter. In the anthers of winter flower buds of C. annuum var, acuminatum the pollen grains show nuclear multiplication and pollens with all nuclear numbers between two and eight are represented. However, 'pollen embryo sacs' as such are not formed. The normal viable pollen grains are binucleate. The pollen fertility in summer is 93.6% while in winter, on the same plants, it is only 31.3 %. In the development of the ovule there are no marked differences amongst the varieties except in size. Coexistence of mono-, bi- and tetrasporic embryo sac developments has been found in C. annuum var. acuminatum in which monospory is dominant in summer but bispory in winter. Monosporic and bisporic embryo sacs coexist in C. baccatum var. pendulum and C. frutescens var. baccatum. The other varieties show only monosporic development.

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