Replication of the rRNA and legumin genes in synchronized root cells of pea (Pisum sativum): evidence for transient EcoR I sites in replicating rRNA genes

1987 ◽  
Vol 8 (2) ◽  
pp. 133-143 ◽  
Author(s):  
J. Van't Hof ◽  
P. Hernandez ◽  
C. A. Bjerknes ◽  
E. K. Kraszewska ◽  
S. S. Lamm
Keyword(s):  
Pisum Sativum ◽  
Rrna Genes ◽  
Root Cells

scholarly journals Synchrotron Radiation Spectroscopy and Transmission Electron Microscopy Techniques to Evaluate TiO2 NPs Incorporation, Speciation, and Impact on Root Cells Ultrastructure of Pisum sativum L. Plants

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 921
Author(s):  
Simonetta Muccifora ◽  
Hiram Castillo-Michel ◽  
Francesco Barbieri ◽  
Lorenza Bellani ◽  
Monica Ruffini Castiglione ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Synchrotron Radiation ◽  
Pisum Sativum ◽  
Emission Spectrometry ◽  
Root Cells ◽  
Tio2 Nps ◽  
X Ray ◽  
Pisum Sativum L ◽  
Transmission Electron

Biosolids (Bs) for use in agriculture are an important way for introducing and transferring TiO2 nanoparticles (NPs) to plants and food chain. Roots of Pisum sativum L. plants grown in Bs-amended soils spiked with TiO2 800 mg/kg as rutile NPs, anatase NPs, mixture of both NPs and submicron particles (SMPs) were investigated by Transmission Electron Microscopy (TEM), synchrotron radiation based micro X-ray Fluorescence and micro X-ray Absorption Near-Edge Structure (µXRF/µXANES) and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). TEM analysis showed damages in cells ultrastructure of all treated samples, although a more evident effect was observed with single anatase or rutile NPs treatments. Micro-XRF and TEM evidenced the presence of nano and SMPs mainly in the cortex cells near the rhizodermis. Micro-XRF/micro-XANES analysis revealed anatase, rutile, and ilmenite as the main TiO2 polymorphs in the original soil and Bs, and the preferential anatase uptake by the roots. For all treatments Ti concentration in the roots increased by 38–56%, however plants translocation factor (TF) increased mostly with NPs treatment (261–315%) and less with SMPs (about 85%), with respect to control. In addition, all samples showed a limited transfer of TiO2 to the shoots (very low TF value). These findings evidenced a potential toxicity of TiO2 NPs present in Bs and accumulating in soil, suggesting the necessity of appropriate regulations for the occurrence of NPs in Bs used in agriculture.

Accumulation of lead in root cells of Pisum sativum

2008 ◽  
Vol 30 (5) ◽  
pp. 629-637 ◽  
Author(s):  
Arleta Małecka ◽  
Aneta Piechalak ◽  
Iwona Morkunas ◽  
Barbara Tomaszewska
Keyword(s):  
Pisum Sativum ◽  
Root Cells

scholarly journals The formation of symbiotic interface in root nodules of Pisum sativum L. and Medicago truncatula Gaertn

2020 ◽  
Author(s):  
A. V. Tsyganova ◽  
E. V. Seliverstova ◽  
N. J. Brewin ◽  
V. E. Tsyganov
Keyword(s):  
Pisum Sativum ◽  
Medicago Truncatula ◽  
Root Nodules ◽  
Nodule Development ◽  
Root Cells ◽  
Pisum Sativum L ◽  
Species Specific

The infection of root cells of legumes with rhizobia involves the gradual remodelling of the plant-microbial interface. General and species-specific features of symbiotic interface remodelling during nodule development were demonstrated.

scholarly journals Two distinct transcriptional activities of pea (Pisum sativum) chloroplasts share immunochemically related functional polypeptides

1992 ◽  
Vol 286 (3) ◽  
pp. 833-841 ◽  
Author(s):  
S Lakhani ◽  
N C Khanna ◽  
K K Tewari
Keyword(s):  
Pisum Sativum ◽  
Rna Polymerase ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Mobility Shift ◽  
Molecular Masses ◽  
Transcription In Vitro ◽  
Functional Components ◽  
16 S Rrna

An RNA polymerase activity has been purified from pea (Pisum sativum) chloroplast extracts with a distinct transcriptional specificity for a chloroplast messenger gene. This activity (ms-RNA pol) differs from the pea RNA polymerase preparation reported by Sun, Shapiro, Wu & Tewari [(1986) Plant Mol. Biol. 6, 429-439], which specifically transcribes only the rRNA gene (rb-RNA pol). The specificity of transcription has been assessed by the synthesis in vitro of discrete transcripts of predicted sizes using cloned promoter regions of the chloroplast psbA and 16 S rRNA genes. The ms-RNA pol preparation, with polypeptides ranging in apparent molecular mass from 22 to 180 kDa, correctly initiates transcription from recombinant plasmids containing the psbA promoter and does not support 16 S rRNA promoter-directed transcription. The two activities differ also in their response to Mn2+ ions. To investigate whether the two transcriptional activities share common functional polypeptides, monoclonal antibodies were developed against the rb-RNA pol preparation. Three clones were selected on the basis of their ability to inhibit transcription in vitro of the 16 S rRNA gene by rb-RNA pol. The antibodies from these clones independently recognized three polypeptides with molecular masses of 27, 90 and 95 kDa on immunoblots. Antibodies cross-reacting with the 90 kDa polypeptide completely eliminated the specific retardation of an end-labelled 16 S rRNA promoter fragment in a mobility-shift assay, whereas the antibodies against the 95 kDa polypeptide resulted in the formation of a ternary complex (enzyme-DNA-antibody). The antibodies cross-reacting with the 27 kDa polypeptide, however, did not alter the mobility of the retarded DNA-enzyme complex on the gel. These antibodies also inhibited transcription in vitro of the psbA gene by ms-RNA pol and recognized polypeptides of identical molecular masses in the ms-RNA pol. These results show that the three polypeptides are functional components of the chloroplast transcriptional complex and appear to be involved in the transcription of both rRNA and mRNA genes. Transcriptional specificity is probably conferred by ancillary transcription factor(s) which remain to be identified.

scholarly journals RIBOSOMAL DNA AMOUNTS IN PISUM SATIVUM

Genetics ◽  
1975 ◽  
Vol 81 (3) ◽  
pp. 485-492
Author(s):  
C A Cullis ◽  
D Roy Davies
Keyword(s):  
Pisum Sativum ◽  
Ribosomal Dna ◽  
Dna Content ◽  
Leaf Size ◽  
Rrna Genes ◽  
Root Tips ◽  
The One ◽  
Cotyledon Cells ◽  
Dna Amounts

ABSTRACT Different varieties of peas have different proportions of rDNA in their genomes; there is no obvious correlation between the proportions and seed or leaf size. The rDNA proportions in root tips, seedlings, leaves and in the cotyledon cells of high DNA content, were compared in four varieties. In three, there was no difference between tissues; the fourth showed an amplification of rRNA genes in the cells of high DNA content of the seed cotyledon, and also in the cells of young but not of older leaves. The fourth variety was the one that had the lowest proportion of rDNA of all those examined. Studies of the tissues of hybrids between genotypes with "low" and "high" proportions of rDNA showed that heterozygotes had the "high" value, showing again the occurrence of an amplification phenomenon.

scholarly journals Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
May Labeeb ◽  
Abdelfattah Badr ◽  
Soliman A. Haroun ◽  
Magdy Z. Mattar ◽  
Aziza S. El-kholy
Keyword(s):  
Silver Nanoparticles ◽  
Pisum Sativum ◽  
Point Mutations ◽  
Cell Ultrastructure ◽  
Root Cells ◽  
Transmission Electron ◽  
Inter Simple Sequence Repeats ◽  
Low Concentrations ◽  
The Impact ◽  
Simple Sequence

Abstract Background Silver nanoparticles (AgNPs) are the most widely used nanomaterial in agricultural and environmental applications. In this study, the impact of AgNPs solutions at 20 mg/L, 40 mg/L, 80 mg/L, and 160 mg/L on cell ultrastructure have been examined in pea (Pisum sativum L) using a transmission electron microscope (TEM). The effect of AgNPs treatments on the α, β esterase (EST), and peroxidase (POX) enzymes expression as well as gain or loss of inter-simple sequence repeats (ISSRs) markers has been described. Results Different structural malformations in the cell wall and mitochondria, as well as plasmolysis and vacuolation were recorded in root cells. Damaged chloroplast and mitochondria were frequently observed in leaves and the osmiophilic plastoglobuli were more observed as AgNPs concentration increased. Starch grains increased by the treatment with 20 mg/L AgNPs. The expressions of α, β EST, and POX were slightly changed but considerable polymorphism in ISSR profiles, using 17 different primers, were scored indicating gain or loss of gene loci as a result of AgNPs treatments. This indicates considerable variations in genomic DNA and point mutations that may be induced by AgNPs as a genotoxic nanomaterial. Conclusion AgNPs may be used to induce genetic variation at low concentrations. However, considerations should be given to the uncontrolled use of nanoparticles and calls for evaluating their impact on plant growth and potential genotoxicity are justified.

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