Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography

1987 ◽  
Vol 9 (2) ◽  
pp. 77-86 ◽  
Author(s):  
J. Van't Hof ◽  
S. S. Lamm ◽  
C. A. Bjerknes
Keyword(s):  
Pisum Sativum ◽  
Deae Cellulose ◽  
Replication Initiation ◽  
Root Cells

scholarly journals A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves

1981 ◽  
Vol 193 (1) ◽  
pp. 375-378 ◽  
Author(s):  
A R Ashton ◽  
L E Anderson
Keyword(s):  
Ionic Strength ◽  
Pisum Sativum ◽  
Deae Cellulose ◽  
High Concentrations ◽  
Rapid Purification ◽  
Low Ionic Strength

Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.

scholarly journals Synchrotron Radiation Spectroscopy and Transmission Electron Microscopy Techniques to Evaluate TiO2 NPs Incorporation, Speciation, and Impact on Root Cells Ultrastructure of Pisum sativum L. Plants

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 921
Author(s):  
Simonetta Muccifora ◽  
Hiram Castillo-Michel ◽  
Francesco Barbieri ◽  
Lorenza Bellani ◽  
Monica Ruffini Castiglione ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Synchrotron Radiation ◽  
Pisum Sativum ◽  
Emission Spectrometry ◽  
Root Cells ◽  
Tio2 Nps ◽  
X Ray ◽  
Pisum Sativum L ◽  
Transmission Electron

Biosolids (Bs) for use in agriculture are an important way for introducing and transferring TiO2 nanoparticles (NPs) to plants and food chain. Roots of Pisum sativum L. plants grown in Bs-amended soils spiked with TiO2 800 mg/kg as rutile NPs, anatase NPs, mixture of both NPs and submicron particles (SMPs) were investigated by Transmission Electron Microscopy (TEM), synchrotron radiation based micro X-ray Fluorescence and micro X-ray Absorption Near-Edge Structure (µXRF/µXANES) and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). TEM analysis showed damages in cells ultrastructure of all treated samples, although a more evident effect was observed with single anatase or rutile NPs treatments. Micro-XRF and TEM evidenced the presence of nano and SMPs mainly in the cortex cells near the rhizodermis. Micro-XRF/micro-XANES analysis revealed anatase, rutile, and ilmenite as the main TiO2 polymorphs in the original soil and Bs, and the preferential anatase uptake by the roots. For all treatments Ti concentration in the roots increased by 38–56%, however plants translocation factor (TF) increased mostly with NPs treatment (261–315%) and less with SMPs (about 85%), with respect to control. In addition, all samples showed a limited transfer of TiO2 to the shoots (very low TF value). These findings evidenced a potential toxicity of TiO2 NPs present in Bs and accumulating in soil, suggesting the necessity of appropriate regulations for the occurrence of NPs in Bs used in agriculture.

Replication of the rRNA and legumin genes in synchronized root cells of pea (Pisum sativum): evidence for transient EcoR I sites in replicating rRNA genes

1987 ◽  
Vol 8 (2) ◽  
pp. 133-143 ◽  
Author(s):  
J. Van't Hof ◽  
P. Hernandez ◽  
C. A. Bjerknes ◽  
E. K. Kraszewska ◽  
S. S. Lamm
Keyword(s):  
Pisum Sativum ◽  
Rrna Genes ◽  
Root Cells

Accumulation of lead in root cells of Pisum sativum

2008 ◽  
Vol 30 (5) ◽  
pp. 629-637 ◽  
Author(s):  
Arleta Małecka ◽  
Aneta Piechalak ◽  
Iwona Morkunas ◽  
Barbara Tomaszewska
Keyword(s):  
Pisum Sativum ◽  
Root Cells

scholarly journals The formation of symbiotic interface in root nodules of Pisum sativum L. and Medicago truncatula Gaertn

2020 ◽  
Author(s):  
A. V. Tsyganova ◽  
E. V. Seliverstova ◽  
N. J. Brewin ◽  
V. E. Tsyganov
Keyword(s):  
Pisum Sativum ◽  
Medicago Truncatula ◽  
Root Nodules ◽  
Nodule Development ◽  
Root Cells ◽  
Pisum Sativum L ◽  
Species Specific

The infection of root cells of legumes with rhizobia involves the gradual remodelling of the plant-microbial interface. General and species-specific features of symbiotic interface remodelling during nodule development were demonstrated.

Plant Peptidoglucans Affecting the Phenotypic Expression of Rhizobial Nitrogenase

1980 ◽  
Vol 7 (3) ◽  
pp. 251 ◽  
Author(s):  
R Storey ◽  
M Reporter
Keyword(s):  
Nitrogenase Activity ◽  
Phenotypic Expression ◽  
Deae Cellulose ◽  
H2 Production ◽  
Rhizobium Japonicum ◽  
Small Peptide ◽  
Root Cells ◽  
Rhizobium Strains ◽  
Hydrolysis Of

Dialysable substances capable of influencing rhizobial nitrogenase activity in vitro were obtained from Glycine max root cells during transfilter coculture with Rhizobium japonicum. These substances from the liquid plant-conditioned medium were chromatographed on Sephadex G-25, DEAE- cellulose and carboxymethylcellulose and on a concanavalin A-Sepharose column. The separated active column fractions initiated the phenotypic expression of nitrogenase activity (C2H2 reduction, H2 production) in different Rhizobium strains. Hydrolysis of these column fractions showed them to contain a small peptide and a glucan. Analysis of active fractions also showed the presence of bound copper. It was concluded that the plant fractions involved in stimulating rhizobial nitrogenase activity were peptidoglucans; at least one active fraction may also be a copper metallothionein.

scholarly journals Purification and characterization of N-acetylglutamate 5-phosphotransferase from pea (Pisum sativum) cotyledons

1981 ◽  
Vol 195 (1) ◽  
pp. 71-81 ◽  
Author(s):  
G McKay ◽  
P D Shargool
Keyword(s):  
Pisum Sativum ◽  
Polyacrylamide Gel Electrophoresis ◽  
Deae Cellulose ◽  
Kinetic Studies ◽  
Exchange Chromatography ◽  
Purification And Characterization ◽  
Coomassie Blue ◽  
Km Value ◽  
Acetylglutamate Kinase

N-Acetylglutamate 5-phosphotransferase (acetylglutamate kinase, EC 2.7.2.8) has been isolated from pea (Pisum sativum) cotyledons and purified 312-fold by using heat treatment, (NH4)2SO4 fractionation, affinity chromatography on ATP--Sepharose and ion-exchange chromatography on DEAE-cellulose. This preparation was shown on polyacrylamide-gel electrophoresis to yield one band staining with Coomassie Blue. The enzyme was shown by a variety of techniques to be composed of two different kinds of subunits, of mol.wts. 43000 and 53000 respectively. These subunits are arranged to give either a dimeric or tetrameric enzyme composed of equal numbers of each type of subunit. The dimeric and tetrameric enzyme forms are thought to be interconvertible, the equilibrium between these forms being influenced by the type of ligand bound to the subunits. Kinetic studies performed on the purified enzyme, indicated a random Bi Bi type of mechanism. The enzyme displayed apparent negative co-operativity with respect to one of its substrates, N-acetylglutamate; as a result, two Km values were found for this substrate, one at 1.9 X 10(-3) M and the other at 6.2 X 10(-3) M. A single Km value for ATP was found to be 1.7 X 10(-3) M. Allosteric regulation by arginine was also shown. A model, based on the Koshland, Némethy & Filmer [(1966) Biochemistry 5, 365-385] Sequential model, which adequately describes the kinetic and structural properties of N-acetylglutamate 5-phosphotransferase, is presented.

scholarly journals Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
May Labeeb ◽  
Abdelfattah Badr ◽  
Soliman A. Haroun ◽  
Magdy Z. Mattar ◽  
Aziza S. El-kholy
Keyword(s):  
Silver Nanoparticles ◽  
Pisum Sativum ◽  
Point Mutations ◽  
Cell Ultrastructure ◽  
Root Cells ◽  
Transmission Electron ◽  
Inter Simple Sequence Repeats ◽  
Low Concentrations ◽  
The Impact ◽  
Simple Sequence

Abstract Background Silver nanoparticles (AgNPs) are the most widely used nanomaterial in agricultural and environmental applications. In this study, the impact of AgNPs solutions at 20 mg/L, 40 mg/L, 80 mg/L, and 160 mg/L on cell ultrastructure have been examined in pea (Pisum sativum L) using a transmission electron microscope (TEM). The effect of AgNPs treatments on the α, β esterase (EST), and peroxidase (POX) enzymes expression as well as gain or loss of inter-simple sequence repeats (ISSRs) markers has been described. Results Different structural malformations in the cell wall and mitochondria, as well as plasmolysis and vacuolation were recorded in root cells. Damaged chloroplast and mitochondria were frequently observed in leaves and the osmiophilic plastoglobuli were more observed as AgNPs concentration increased. Starch grains increased by the treatment with 20 mg/L AgNPs. The expressions of α, β EST, and POX were slightly changed but considerable polymorphism in ISSR profiles, using 17 different primers, were scored indicating gain or loss of gene loci as a result of AgNPs treatments. This indicates considerable variations in genomic DNA and point mutations that may be induced by AgNPs as a genotoxic nanomaterial. Conclusion AgNPs may be used to induce genetic variation at low concentrations. However, considerations should be given to the uncontrolled use of nanoparticles and calls for evaluating their impact on plant growth and potential genotoxicity are justified.

Sign in / Sign up

Export Citation Format

Share Document