Kluyveromyces lactis killer plasmid pGKL2: Molecular analysis of an essential gene, ORF5

Yeast ◽  
1995 ◽  
Vol 11 (7) ◽  
pp. 615-628 ◽  
Author(s):  
Raffael Schaffrath ◽  
Peter A. Meacock
Keyword(s):  
Molecular Analysis ◽  
Essential Gene ◽  
Kluyveromyces Lactis ◽  
Killer Plasmid

Heterologous gene expression on the linear DNA killer plasmid from Kluyveromyces lactis

1991 ◽  
Vol 19 (2) ◽  
pp. 109-118 ◽  
Author(s):  
J. K�mper ◽  
K. Esser ◽  
N. Gunge ◽  
F. Meinhardt
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Kluyveromyces Lactis ◽  
Heterologous Gene ◽  
Linear Dna ◽  
Killer Plasmid

scholarly journals Molecular analysis of an essential gene upstream ofrpoNinRhizobiumNGR234

1991 ◽  
Vol 82 (2) ◽  
pp. 195-201
Author(s):  
Jan-Christoph Slooten ◽  
John Stanley
Keyword(s):  
Molecular Analysis ◽  
Essential Gene

scholarly journals Transcription apparatus of the yeast killer DNA plasmids: Architecture, function, and evolutionary origin

2018 ◽  
Author(s):  
Michal Sýkora ◽  
Martin Pospíšek ◽  
Josef Novák ◽  
Silvia Mrvová ◽  
Libor Krásný ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Kluyveromyces Lactis ◽  
Evolutionary Origin ◽  
Structural Elements ◽  
Extrachromosomal Elements ◽  
Mrna Capping ◽  
Capping Enzyme ◽  
Killer Plasmid ◽  
Yeast Killer

ABSTRACTTranscription of extrachromosomal elements such as organelles, viruses, and plasmids is dependent on cellular RNA polymerase (RNAP) or intrinsic RNAP encoded by these elements. The yeastKluyveromyces lactiscontains killer DNA plasmids that bear putative non-canonical RNAP genes. Here, we describe the architecture and evolutionary origin of this transcription machinery. We show that the two RNAP subunits interactin vivo, and this complex interacts with another two plasmid-encoded proteins - the mRNA capping enzyme, and a putative helicase which interacts with plasmid-specific DNA. Further, we identify a promoter element that causes 5’ polyadenylation of plasmid-specific transcriptsviaRNAP slippage during transcription initiation, and structural elements that precede the termination sites. As a result, we present a first model of the yeast killer plasmid transcription initiation and intrinsic termination. Finally, we demonstrate that plasmid RNAP and its promoters display high similarity to poxviral RNAP and promoters of early poxviral genes, respectively.

Isolation and molecular analysis of the gene for cytochrome c 1 from Kluyveromyces lactis

1996 ◽  
Vol 30 (2) ◽  
pp. 145-150 ◽  
Author(s):  
Yvetta Gbelsk� ◽  
Katar�na Horv�thov� ◽  
Quirina J. M. van der Aart ◽  
B. J. M. Zonneveld ◽  
H. Y. Steensma ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Molecular Analysis ◽  
Kluyveromyces Lactis

scholarly journals Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis

1988 ◽  
Vol 16 (13) ◽  
pp. 5863-5878 ◽  
Author(s):  
M. Tommasino ◽  
S. Ricci ◽  
C.L. Galeotti
Keyword(s):  
Genome Organization ◽  
Kluyveromyces Lactis ◽  
Killer Plasmid

scholarly journals Transformation of Kluyveromyces lactis by killer plasmid DNA.

1983 ◽  
Vol 154 (2) ◽  
pp. 737-742 ◽  
Author(s):  
L de Louvencourt ◽  
H Fukuhara ◽  
H Heslot ◽  
M Wesolowski
Keyword(s):  
Plasmid Dna ◽  
Kluyveromyces Lactis ◽  
Killer Plasmid
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