Yeast killer plasmid pGKL2: molecular analysis of UCS5, a cytoplasmic promoter element essential for ORF5 gene function

1996 ◽  
Vol 250 (3) ◽  
pp. 286-294 ◽  
Author(s):  
R. Schaffrath ◽  
P. A. Meacock ◽  
F. Meinhardt
Keyword(s):  
Molecular Analysis ◽  
Gene Function ◽  
Promoter Element ◽  
Orf5 Gene ◽  
Killer Plasmid ◽  
Yeast Killer

Yeast killer plasmid mutations affecting toxin secretion and activity and toxin immunity function

1982 ◽  
Vol 2 (4) ◽  
pp. 346-354
Author(s):  
H Bussey ◽  
W Sacks ◽  
D Galley ◽  
D Saville
Keyword(s):  
Molecular Weight ◽  
In Vitro Translation ◽  
Heat Curing ◽  
In The Wild ◽  
Toxin Secretion ◽  
Killer Plasmid ◽  
Yeast Killer ◽  
Immunity Function

M double-stranded RNA (MdsRNA) plasmid mutants were obtained by mutagenesis and screening of a diploid killer culture partially heat cured of the plasmid, so that a high proportion of the cells could be expected to have only on M plasmid. Mutants with neutral (nonkiller [K-], immune [R+]) or suicide (killer [K+], sensitive [R-] phenotypes were examined. All mutants became K- R- sensitives on heat curing of the MdsRNA plasmid, and showed cytoplasmic inheritance by random spore analysis. In some cases, M plasmid mutations were indicated by altered mobility of the MdsRNA by agarose gel electrophoresis or by altered size of in vitro translation products from denatured dsRNA. Neutral mutants were of two types: nonsecretors of the toxin protein or secretors of an inactive toxin. Of three neutral nonsecretors examined, one (NLP-1), probably a nonsense mutation, made a smaller protoxin precursor in vitro and in vivo, and two made full-size protoxin molecules. The in vivo protoxin of 43,000 molecular weight was unstable in the wild type and kinetically showed a precursor-product relationship to the processed, secreted 11,000-molecular-weight toxin. In one nonsecretor (N1), the protoxin appeared more stable in a pulse-chase experiment, and could be altered in a recognition site required for protein processing.

scholarly journals yeast killer plasmid pGKL1 encodes a DNA polymerase belonging to the family B DNA polymerases

1987 ◽  
Vol 15 (21) ◽  
pp. 9088-9088 ◽  
Author(s):  
Guhung Jung ◽  
Mark C. Leavitt ◽  
Junetsu Ito
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerases ◽  
The Family ◽  
Killer Plasmid ◽  
Yeast Killer

Kluyveromyces lactis killer plasmid pGKL2: Molecular analysis of an essential gene, ORF5

Yeast ◽  
1995 ◽  
Vol 11 (7) ◽  
pp. 615-628 ◽  
Author(s):  
Raffael Schaffrath ◽  
Peter A. Meacock
Keyword(s):  
Molecular Analysis ◽  
Essential Gene ◽  
Kluyveromyces Lactis ◽  
Killer Plasmid

scholarly journals Transcription apparatus of the yeast killer DNA plasmids: Architecture, function, and evolutionary origin

2018 ◽  
Author(s):  
Michal Sýkora ◽  
Martin Pospíšek ◽  
Josef Novák ◽  
Silvia Mrvová ◽  
Libor Krásný ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Kluyveromyces Lactis ◽  
Evolutionary Origin ◽  
Structural Elements ◽  
Extrachromosomal Elements ◽  
Mrna Capping ◽  
Capping Enzyme ◽  
Killer Plasmid ◽  
Yeast Killer

ABSTRACTTranscription of extrachromosomal elements such as organelles, viruses, and plasmids is dependent on cellular RNA polymerase (RNAP) or intrinsic RNAP encoded by these elements. The yeastKluyveromyces lactiscontains killer DNA plasmids that bear putative non-canonical RNAP genes. Here, we describe the architecture and evolutionary origin of this transcription machinery. We show that the two RNAP subunits interactin vivo, and this complex interacts with another two plasmid-encoded proteins - the mRNA capping enzyme, and a putative helicase which interacts with plasmid-specific DNA. Further, we identify a promoter element that causes 5’ polyadenylation of plasmid-specific transcriptsviaRNAP slippage during transcription initiation, and structural elements that precede the termination sites. As a result, we present a first model of the yeast killer plasmid transcription initiation and intrinsic termination. Finally, we demonstrate that plasmid RNAP and its promoters display high similarity to poxviral RNAP and promoters of early poxviral genes, respectively.

scholarly journals Yeast killer plasmid mutations affecting toxin secretion and activity and toxin immunity function.

1982 ◽  
Vol 2 (4) ◽  
pp. 346-354 ◽  
Author(s):  
H Bussey ◽  
W Sacks ◽  
D Galley ◽  
D Saville
Keyword(s):  
Molecular Weight ◽  
In Vitro Translation ◽  
Heat Curing ◽  
In The Wild ◽  
Toxin Secretion ◽  
Killer Plasmid ◽  
Yeast Killer ◽  
Immunity Function

M double-stranded RNA (MdsRNA) plasmid mutants were obtained by mutagenesis and screening of a diploid killer culture partially heat cured of the plasmid, so that a high proportion of the cells could be expected to have only on M plasmid. Mutants with neutral (nonkiller [K-], immune [R+]) or suicide (killer [K+], sensitive [R-] phenotypes were examined. All mutants became K- R- sensitives on heat curing of the MdsRNA plasmid, and showed cytoplasmic inheritance by random spore analysis. In some cases, M plasmid mutations were indicated by altered mobility of the MdsRNA by agarose gel electrophoresis or by altered size of in vitro translation products from denatured dsRNA. Neutral mutants were of two types: nonsecretors of the toxin protein or secretors of an inactive toxin. Of three neutral nonsecretors examined, one (NLP-1), probably a nonsense mutation, made a smaller protoxin precursor in vitro and in vivo, and two made full-size protoxin molecules. The in vivo protoxin of 43,000 molecular weight was unstable in the wild type and kinetically showed a precursor-product relationship to the processed, secreted 11,000-molecular-weight toxin. In one nonsecretor (N1), the protoxin appeared more stable in a pulse-chase experiment, and could be altered in a recognition site required for protein processing.

Molecular analysis of neurotransmitter release

1998 ◽  
Vol 33 ◽  
pp. 29-41 ◽  
Author(s):  
Giampietro Schiavo ◽  
Gudrun Stenbeck
Keyword(s):  
Molecular Analysis ◽  
Neurotransmitter Release

Molecular analysis of the lissencephaly gene 1 (LIS-1) in medulloblastomas

1996 ◽  
Vol 22 (3) ◽  
pp. 233-242 ◽  
Author(s):  
A. Koch ◽  
J. Tonn ◽  
J. A. Kraus ◽  
N. Sarensen ◽  
S. Albrecht ◽  
...  
Keyword(s):  
Molecular Analysis
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