Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1800698 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cytokine Secretion ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

scholarly journals Single Cell Analysis: Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion (Small 26/2018)

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1870119 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cytokine Secretion ◽  
Cell Analysis ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

A Novel Imaging Approach for Single-Cell Real-Time Analysis of Oncolytic Virus Replication and Efficacy in Cancer Cells

2021 ◽  
Vol 32 (3-4) ◽  
pp. 166-177
Author(s):  
Lorraine Quillien ◽  
Sokunthea Top ◽  
Sandrine Kappler-Gratias ◽  
Agathe Redouté ◽  
Nelson Dusetti ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Real Time ◽  
Virus Replication ◽  
Oncolytic Virus ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Imaging Approach

scholarly journals Quantifying single-cell secretion in real time using resonant hyperspectral imaging

2018 ◽  
Vol 115 (52) ◽  
pp. 13204-13209 ◽  
Author(s):  
José Juan-Colás ◽  
Ian S. Hitchcock ◽  
Mark Coles ◽  
Steven Johnson ◽  
Thomas F. Krauss
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Cell Communication ◽  
Label Free ◽  
Cell Secretion ◽  
Physiological Disorder ◽  
Real Time Analysis ◽  
Technology Heterogeneity

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

Protein Microarray Copying: Easy on‐Demand Protein Microarray Generation Compatible with Fluorescence and Label‐Free Real‐Time Analysis

ChemBioChem ◽  
2019 ◽  
Author(s):  
Normann Kilb ◽  
Tobias Herz ◽  
Jürgen Burger ◽  
Johannes Woehrle ◽  
Philipp A. Meyer ◽  
...  
Keyword(s):  
Real Time ◽  
Protein Microarray ◽  
Label Free ◽  
Time Analysis ◽  
On Demand ◽  
Real Time Analysis

Plasmonic nanohole array biosensor for label-free and real-time analysis of live cell secretion

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2208-2217 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Cenk I. Özdemir ◽  
Alexander Belushkin ◽  
Filiz Yesilköy ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Real Time ◽  
Live Cell ◽  
Label Free ◽  
Cell Secretion ◽  
Time Analysis ◽  
Nanohole Array ◽  
Real Time Analysis ◽  
Array Biosensor

A new microfluidic-integrated nanoplasmonic biosensor for the study of cell signaling in a label-free and real-time manner.

scholarly journals Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

Micromachines ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 107 ◽  
Author(s):  
Chen Zhu ◽  
Xi Luo ◽  
Wilfred Villariza Espulgar ◽  
Shohei Koyama ◽  
Atsushi Kumanogoh ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Clinical Diagnostics ◽  
Cytokine Secretion ◽  
Localized Surface Plasmon ◽  
Single Cell Level ◽  
Label Free ◽  
Real Time Monitoring ◽  
Cell Level

Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.

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