A Novel Imaging Approach for Single-Cell Real-Time Analysis of Oncolytic Virus Replication and Efficacy in Cancer Cells

2021 ◽  
Vol 32 (3-4) ◽  
pp. 166-177
Author(s):  
Lorraine Quillien ◽  
Sokunthea Top ◽  
Sandrine Kappler-Gratias ◽  
Agathe Redouté ◽  
Nelson Dusetti ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Real Time ◽  
Virus Replication ◽  
Oncolytic Virus ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Imaging Approach

scholarly journals Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics

2019 ◽  
Author(s):  
Nicole C. Robb ◽  
Aartjan J.W. te Velthuis ◽  
Ervin Fodor ◽  
Achillefs N. Kapanidis
Keyword(s):  
Real Time ◽  
Virus Replication ◽  
Dynamic Equilibrium ◽  
Influenza Viruses ◽  
Time Analysis ◽  
Template Strand ◽  
Real Time Analysis ◽  
Influenza Virus Replication ◽  
Multiple Conformations

ABSTRACTThe viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; further, little is known about cRNA promoter conformations. To probe the RNA conformations adopted during initial replication, we monitored single, surface-immobilised vRNA and cRNA initiation complexes in real-time. Our results show that, while the 3’ terminus of the vRNA promoter exists in dynamic equilibrium between pre-initiation and initiation conformations, the cRNA promoter exhibited very limited dynamics. Two residues in the proximal 3’ region of the cRNA promoter (residues absent in the vRNA promoter) allowed the cRNA template strand to reach further into the active site, limiting promoter dynamics. Our results highlight promoter-dependent differences in influenza initiation mechanisms, and advance our understanding of virus replication.

scholarly journals Single Cell Analysis: Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion (Small 26/2018)

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1870119 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cytokine Secretion ◽  
Cell Analysis ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1800698 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cytokine Secretion ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

scholarly journals Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics

2019 ◽  
Vol 47 (12) ◽  
pp. 6466-6477 ◽  
Author(s):  
Nicole C Robb ◽  
Aartjan J W te Velthuis ◽  
Ervin Fodor ◽  
Achillefs N Kapanidis
Keyword(s):  
Real Time ◽  
Virus Replication ◽  
Dynamic Equilibrium ◽  
Influenza Viruses ◽  
Time Analysis ◽  
Template Strand ◽  
Real Time Analysis ◽  
Influenza Virus Replication ◽  
Multiple Conformations

AbstractThe viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; further, little is known about cRNA promoter conformations. To probe the RNA conformations adopted during initial replication, we monitored single, surface-immobilized vRNA and cRNA initiation complexes in real-time. Our results show that, while the 3′ terminus of the vRNA promoter exists in dynamic equilibrium between pre-initiation and initiation conformations, the cRNA promoter exhibited very limited dynamics. Two residues in the proximal 3′ region of the cRNA promoter (residues absent in the vRNA promoter) allowed the cRNA template strand to reach further into the active site, limiting promoter dynamics. Our results highlight promoter-dependent differences in influenza initiation mechanisms, and advance our understanding of virus replication.

scholarly journals Real-time analysis of the effects of toxic, therapeutic and sub-therapeutic concentrations of digitoxin on lung cancer cells

2014 ◽  
Vol 59 ◽  
pp. 192-199 ◽  
Author(s):  
R. Eldawud ◽  
T.A. Stueckle ◽  
S. Manivannan ◽  
H. Elbaz ◽  
M. Chen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Lung Cancer Cells ◽  
Time Analysis ◽  
Real Time Analysis

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

scholarly journals Real-Time Analysis of Animal Feeding Behavior With a Low-Calculation-Power CPU

2020 ◽  
Vol 67 (4) ◽  
pp. 1197-1205 ◽  
Author(s):  
Yuki Totani ◽  
Susumu Kotani ◽  
Kei Odai ◽  
Etsuro Ito ◽  
Manabu Sakakibara
Keyword(s):  
Feeding Behavior ◽  
Real Time ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Animal Feeding
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