scholarly journals Single Cell Analysis: Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion (Small 26/2018)

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1870119 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cytokine Secretion ◽  
Cell Analysis ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1800698 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cytokine Secretion ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

A Novel Imaging Approach for Single-Cell Real-Time Analysis of Oncolytic Virus Replication and Efficacy in Cancer Cells

2021 ◽  
Vol 32 (3-4) ◽  
pp. 166-177
Author(s):  
Lorraine Quillien ◽  
Sokunthea Top ◽  
Sandrine Kappler-Gratias ◽  
Agathe Redouté ◽  
Nelson Dusetti ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Real Time ◽  
Virus Replication ◽  
Oncolytic Virus ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Imaging Approach

scholarly journals Quantifying single-cell secretion in real time using resonant hyperspectral imaging

2018 ◽  
Vol 115 (52) ◽  
pp. 13204-13209 ◽  
Author(s):  
José Juan-Colás ◽  
Ian S. Hitchcock ◽  
Mark Coles ◽  
Steven Johnson ◽  
Thomas F. Krauss
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Cell Communication ◽  
Label Free ◽  
Cell Secretion ◽  
Physiological Disorder ◽  
Real Time Analysis ◽  
Technology Heterogeneity

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

High-Throughput, Long-Term Imaging of Salmonella Infecting Macrophages in a Micro-Environmental System

2006 ◽  
Author(s):  
Shih-Hui Chao ◽  
Tim J. Strovas ◽  
Ting-She M. Wang ◽  
Kendan A. Jones-Isaac ◽  
Susan L. Fink ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Laser Scanning ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Cellular Functions ◽  
Laser Scanning Confocal Microscope ◽  
History Of ◽  
Multiple Variables

Real-time single cell analysis is necessary to understand dynamic cellular functions in time and space. Such analyses require the simultaneous measurement of multiple variables in real-time, due to heterogeneity in cellular populations. We report the application of using a micro-environmental chamber on an automatic laser scanning confocal microscope to observe murine macrophage cells in incubation conditions for more than 18 hours. The motorized stage of the microscope was programmed to scan through pre-defined monitoring locations to increase the observation throughput. The acquired images were post-processed to extract the information of each cell. In contrast to current single-cell technologies, such as fluorescence-activated cell sorter (FACS) based systems, the reported architecture records the history of the physiological responses of individual cells.

scholarly journals Real Time Single Cell Analysis of Bid Cleavage and Bid Translocation during Caspase-dependent and Neuronal Caspase-independent Apoptosis

2005 ◽  
Vol 281 (9) ◽  
pp. 5837-5844 ◽  
Author(s):  
Manus W. Ward ◽  
Markus Rehm ◽  
Heiko Duessmann ◽  
Slavomir Kacmar ◽  
Caoimhin G. Concannon ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cell Analysis
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