Plasmonic nanohole array biosensor for label-free and real-time analysis of live cell secretion

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2208-2217 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Cenk I. Özdemir ◽  
Alexander Belushkin ◽  
Filiz Yesilköy ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Real Time ◽  
Live Cell ◽  
Label Free ◽  
Cell Secretion ◽  
Time Analysis ◽  
Nanohole Array ◽  
Real Time Analysis ◽  
Array Biosensor

A new microfluidic-integrated nanoplasmonic biosensor for the study of cell signaling in a label-free and real-time manner.

scholarly journals Quantifying single-cell secretion in real time using resonant hyperspectral imaging

2018 ◽  
Vol 115 (52) ◽  
pp. 13204-13209 ◽  
Author(s):  
José Juan-Colás ◽  
Ian S. Hitchcock ◽  
Mark Coles ◽  
Steven Johnson ◽  
Thomas F. Krauss
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Cell Communication ◽  
Label Free ◽  
Cell Secretion ◽  
Physiological Disorder ◽  
Real Time Analysis ◽  
Technology Heterogeneity

Cell communication is primarily regulated by secreted proteins, whose inhomogeneous secretion often indicates physiological disorder. Parallel monitoring of innate protein-secretion kinetics from individual cells is thus crucial to unravel systemic malfunctions. Here, we report a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology. Heterogeneity in physiological thrombopoietin expression from individual HepG2 liver cells in response to platelet desialylation was quantified demonstrating how mapping real-time protein secretion can provide a simple, yet powerful approach for studying complex physiological systems regulating protein production at single-cell resolution.

scholarly journals Real-time analysis of endosomal lipid transport by live cell scintillation proximity assay

2004 ◽  
Vol 45 (11) ◽  
pp. 2151-2158 ◽  
Author(s):  
Walter Stockinger ◽  
Adam B. Castoreno ◽  
Yan Wang ◽  
Joanne C. Pagnon ◽  
Axel Nohturfft
Keyword(s):  
Real Time ◽  
Live Cell ◽  
Lipid Transport ◽  
Time Analysis ◽  
Scintillation Proximity Assay ◽  
Real Time Analysis

scholarly journals Single Cell Analysis: Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion (Small 26/2018)

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1870119 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cytokine Secretion ◽  
Cell Analysis ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1800698 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cytokine Secretion ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis

Protein Microarray Copying: Easy on‐Demand Protein Microarray Generation Compatible with Fluorescence and Label‐Free Real‐Time Analysis

ChemBioChem ◽  
2019 ◽  
Author(s):  
Normann Kilb ◽  
Tobias Herz ◽  
Jürgen Burger ◽  
Johannes Woehrle ◽  
Philipp A. Meyer ◽  
...  
Keyword(s):  
Real Time ◽  
Protein Microarray ◽  
Label Free ◽  
Time Analysis ◽  
On Demand ◽  
Real Time Analysis

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

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