scholarly journals Results of a Multicenter Evaluation of a Rapid Flow Cytometry Potency Assay for Cryopreserved Cord Blood Units: A BEST Collaborative Study

2021 ◽  
Vol 10 (S1) ◽  
Author(s):  
Renée Bazin ◽  
Carl Simard ◽  
Diane Fournier ◽  
Patrick Trépanier
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Collaborative Study ◽  
Potency Assay ◽  
Rapid Flow

scholarly journals Optimization of the Aldehyde Dehydrogenase-Based Cord Blood Potency Assay Using Red Blood Cell Depletion

2016 ◽  
Vol 22 (3) ◽  
pp. S424-S425
Author(s):  
Kevin Shoulars ◽  
Tracy Gentry ◽  
Ben Lam ◽  
Bert Wognum ◽  
Steve Szilvassy ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cord Blood ◽  
Aldehyde Dehydrogenase ◽  
Red Blood Cell ◽  
Cell Depletion ◽  
Potency Assay

scholarly journals Cord Blood Extracellular Vesicles Analyzed by Flow Cytometry with Thresholding Using 405 nm or 488 nm Laser Leads to Concurrent Results

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1320
Author(s):  
Kristýna Pekárková ◽  
Jakub Soukup ◽  
Marie Kostelanská ◽  
Jan Širc ◽  
Zbyněk Straňák ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Extracellular Vesicles ◽  
Correlation Coefficients ◽  
Flow Cytometer ◽  
Liquid Biopsies ◽  
Physiological Conditions ◽  
Flow Cytometric ◽  
Term Births ◽  
And Control

Extracellular vesicles (EVs) from liquid biopsies are extensively analyzed by flow cytometry, a technology that is continuously evolving. Thresholding utilizing a violet 405 nm laser side scatter (VSSC) has recently been implemented. Here, we collected set of large EV (lEV) samples from cord blood, which we analyzed using a standard flow cytometer improved via a 405 nm laser side scatter. Samples were analyzed using two distinct thresholding methods—one based on VSSC, and one based on VSSC combined with fluorescence thresholding on stained phosphatidylserine. Through these thresholding methods, we compared lEVs from pre-term births and control cord blood. Double-labeled lEVs with platelet CD36+/CD41+, activated platelet CD41+/CD62P+ and endothelial CD31+/CD105+ antibodies were used. Apart from comparing the two groups together, we also correlated measured lEVs with the thresholding methods. We also correlated the results of this study with data analyzed in our previous study in which we used a conventional 488 nm laser SSC. We did not find any difference between the two cord blood groups. However, we found highly concurrent data via our correlation of the thresholding methods, with correlation coefficients ranging from 0.80 to 0.96 even though the numbers of detected lEVs differed between thresholding methods. In conclusion, our approaches to thresholding provided concurrent data and it seems that improving the cytometer with the use of a VSSC increases its sensitivity, despite not being particularly critical to the validity of flow cytometric studies that compare pathological and physiological conditions in liquid biopsies.

scholarly journals A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Eva Pallinger ◽  
Zoltan Bognar ◽  
Jozsef Bodis ◽  
Timea Csabai ◽  
Nelli Farkas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Embryo Transfer ◽  
Rapid Flow

scholarly journals Development and validation of a rapid, aldehyde dehydrogenase bright–based cord blood potency assay

Blood ◽  
2016 ◽  
Vol 127 (19) ◽  
pp. 2346-2354 ◽  
Author(s):  
Kevin Shoulars ◽  
Pamela Noldner ◽  
Jesse D. Troy ◽  
Lynn Cheatham ◽  
Amanda Parrish ◽  
...  
Keyword(s):  
Cord Blood ◽  
Aldehyde Dehydrogenase ◽  
Blood Bank ◽  
Potency Assay ◽  
Blood Content ◽  
Cord Blood Bank ◽  
Key Points ◽  
Development And Validation

Key Points Cord blood content of ALDHbr cells correlates well with CFUs and may act as a surrogate potency assay for cord blood units. ALDHbr cells in segments are assayed rapidly, allowing potency results to be used for release of the unit from a public cord blood bank.

A rapid flow cytometry method to assess bacterial abundance in agricultural soil

2015 ◽  
Vol 88 ◽  
pp. 60-68 ◽  
Author(s):  
Mélanie Bressan ◽  
Isabelle Trinsoutrot Gattin ◽  
Sylvie Desaire ◽  
Lisa Castel ◽  
Christophe Gangneux ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Agricultural Soil ◽  
Bacterial Abundance ◽  
Rapid Flow

scholarly journals Rapid Flow Cytometry-Based Test for the Diagnosis of Lipopolysaccharide Responsive Beige-Like Anchor (LRBA) Deficiency

2018 ◽  
Vol 9 ◽  
Author(s):  
Laura Gámez-Díaz ◽  
Elena C. Sigmund ◽  
Veronika Reiser ◽  
Werner Vach ◽  
Sophie Jung ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rapid Flow ◽  
Lrba Deficiency
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