Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples

2010 ◽  
Vol 79A (1) ◽  
pp. 14-24 ◽  
Author(s):  
F. D'Alessio ◽  
P. Mirabelli ◽  
M. Gorrese ◽  
G. Scalia ◽  
M. Gemei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Flow Cytometry Analysis ◽  
Human Cord Blood ◽  
Hematopoietic Stem ◽  
Blood Samples ◽  
Polychromatic Flow Cytometry

scholarly journals CD34 Antigen and the MPL Receptor Expression Defines a Novel Class of Human Cord Blood-Derived Primitive Hematopoietic Stem Cells

2017 ◽  
Vol 26 (6) ◽  
pp. 1043-1058 ◽  
Author(s):  
Yoshikazu Matsuoka ◽  
Masaya Takahashi ◽  
Keisuke Sumide ◽  
Hiroshi Kawamura ◽  
Ryusuke Nakatsuka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Receptor Expression ◽  
Human Cord Blood ◽  
Hematopoietic Stem ◽  
Cd34 Antigen

scholarly journals Flow cytometry analysis of murine hematopoietic stem cells

2012 ◽  
Vol 83A (1) ◽  
pp. 27-37 ◽  
Author(s):  
Allison Mayle ◽  
Min Luo ◽  
Mira Jeong ◽  
Margaret A. Goodell
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Hematopoietic Stem Cells ◽  
Flow Cytometry Analysis ◽  
Hematopoietic Stem

Human cord blood-derived primitive CD34-negative hematopoietic stem cells (HSCs) are myeloid-biased long-term HSCs residing at the apex of human HSC hierarchy

2015 ◽  
Vol 43 (9) ◽  
pp. S79 ◽  
Author(s):  
Yoshikazu Matsuoka ◽  
Keisuke Sumide ◽  
Hiroshi Kawamura ◽  
Ryusuke Nakatsuka ◽  
Tatsuya Fujioka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Human Cord Blood ◽  
Hematopoietic Stem

Human hematopoiesis in murine embryos after injecting human cord blood–derived hematopoietic stem cells into murine blastocysts

Blood ◽  
2002 ◽  
Vol 99 (2) ◽  
pp. 719-721 ◽  
Author(s):  
Friedrich Harder ◽  
Reinhard Henschler ◽  
Ilse Junghahn ◽  
Marinus C. Lamers ◽  
Albrecht M. Müller
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Developmental Stages ◽  
Specific Gene ◽  
Human Cord Blood ◽  
Human Donor ◽  
Fetal Sheep ◽  
Hematopoietic Stem ◽  
Murine Embryos

Abstract At different developmental stages, candidate human hematopoietic stem cells (HSCs) are present within the CD34+ CD38− population. By means of xenotransplantation, such CD34+CD38− cells were recently shown to engraft the hematopoietic system of fetal sheep and nonobese diabetic severe combined immunodeficient adult mice. Here it is demonstrated that, after their injection into murine blastocysts, human cord blood (CB)–derived CD34+and CD34+ CD38− cells repopulate the hematopoietic tissues of nonimmunocompromised murine embryos and that human donor contribution can persist to adulthood. It is further observed that human hematopoietic progenitor cells are present in murine hematopoietic tissues of midgestational chimeric embryos and that progeny of the injected human HSCs activate erythroid-specific gene expression. Thus, the early murine embryo provides a suitable environment for the survival and differentiation of human CB CD34+ CD38− cells.

Deregulation of RARγ and RARγ-Specific miRNAs in AML.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3182-3182
Author(s):  
Angela N. Barrett ◽  
Rajeev Gupta ◽  
Annegret Glasow ◽  
David Grimwade ◽  
Marieke von Lindern ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Acute Promyelocytic Leukemia ◽  
Cell Lines ◽  
Promyelocytic Leukemia ◽  
Differentiation Therapy ◽  
Human Cord Blood ◽  
Hematopoietic Stem ◽  
Myeloid Progenitors

Abstract All-trans-retinoic acid (ATRA) plays important regulatory roles in hematopoiesis and is successfully used in differentiation therapy of acute promyelocytic leukemia (APL). Although ATRA effectively inhibits growth and stimulates myelomonocytic differentiation of myeloid progenitors, it is equally potent in causing expansion of multipotent hematopoietic stem cells. Results of studies utilizing mice that lack expression of a specific RAR and/or RAR subtype specific retinoids, as well as work addressing the molecular pathogenesis of acute promyelocytic leukemia (APL), indicate that the effects of ATRA on hematopoietic stem cells and myeloid progenitors are differentially mediated via the RARγ and RARα, respectively. Using qPCR we have now shown that RARγ is expressed in human cord blood derived stem cells but not in more mature myeloid progenitors or myelomonocytic cells. This change in the RARγ expression levels is paralleled by a reciprocal change in expression of RARγ specific miRNA, which we have identified and validated using multiple experimental strategies, including RARγ 3′UTR based reporter assays. RARγ is also expressed in blasts derived from non-APL AML patients (over 80% of cases examined) and AML cell lines, but is not expressed in ATRA responsive APL cell lines. The expression of RARγ miRNA, on the other hand, is markedly decreased in AML blasts when compared to the levels detected in cord blood derived CD34+ myeloid progenitor cells. Taken together our results suggest that finely tuned and miRNA mediated down-regulation of RARγ expression in the myelomonocytic lineage provides a switch from pro-proliferation to RARα mediated pro-differentiation effects of ATRA. We predict that use of a RARα specific agonist, possibly in conjunction with a strategy that negatively targets RARγ (as with RARγ selective antagonist or siRNA), would be most effective in retinoid based differentiation therapy of non APL-AML.

Identification of Single Human Hematopoietic Stem Cells Capable of Long-Term Multilineage Engraftment and Self-Renewal.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 816-816
Author(s):  
Faiyaz Notta ◽  
Sergei Doulatov ◽  
John E. Dick
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Conflicts Of Interest ◽  
Single Cells ◽  
Human Cord Blood ◽  
Hematopoietic Stem ◽  
Human Hscs ◽  
Insight Into

Abstract Abstract 816 A fundamental tenet that has guided our insight into the biology of hematopoietic stem cells (HSCs) over the past 50 years is the principle that an HSC can only be assayed by functional repopulation of an irradiated host1. In its strictest definition, only a HSC can provide long-term reconstitution of all the major lineages following single cell transplantation. However, the existing strategies for human HSC isolation lack quantitation and do not submit to this rigorous standard, thus precluding further biological analysis. Here, we report the prospective and quantitative analysis of human cord blood (CB) HSCs transplanted into female NOD/SCID/IL-2Rgcnull mice. We identify integrin a6 (CD49f) as a novel marker of cord blood (CB) HSCs and report that single Lin-CD34+CD38-CD90+CD45RA-RholoCD49fhi cells can reconstitute myeloid, B-, and T-cell lineages for 18 weeks. 5 of 29 mice transplanted with single cells gave rise to human cells indicating that approximately 20% of cells in this fraction are HSCs. This advance finally enables utilization of near-homogeneous populations of human HSCs to gain insight into their biology and to harness them for stem cell-based therapeutics. Disclosures: No relevant conflicts of interest to declare.

Hypoxia improves expansion potential of human cord blood-derived hematopoietic stem cells and marrow repopulation efficiency

2012 ◽  
Vol 88 (5) ◽  
pp. 396-405 ◽  
Author(s):  
Sushmita Roy ◽  
Manjul Tripathy ◽  
Nitin Mathur ◽  
Asish Jain ◽  
Asok Mukhopadhyay
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Human Cord Blood ◽  
Hematopoietic Stem

scholarly journals GPI-80 expression highly purifies human cord blood–derived primitive CD34-negative hematopoietic stem cells

Blood ◽  
2016 ◽  
Vol 128 (18) ◽  
pp. 2258-2260 ◽  
Author(s):  
Yoshikazu Matsuoka ◽  
Keisuke Sumide ◽  
Hiroshi Kawamura ◽  
Ryusuke Nakatsuka ◽  
Tatsuya Fujioka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Human Cord Blood ◽  
Hematopoietic Stem
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