scholarly journals A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Eva Pallinger ◽  
Zoltan Bognar ◽  
Jozsef Bodis ◽  
Timea Csabai ◽  
Nelli Farkas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Embryo Transfer ◽  
Rapid Flow

A rapid flow cytometry method to assess bacterial abundance in agricultural soil

2015 ◽  
Vol 88 ◽  
pp. 60-68 ◽  
Author(s):  
Mélanie Bressan ◽  
Isabelle Trinsoutrot Gattin ◽  
Sylvie Desaire ◽  
Lisa Castel ◽  
Christophe Gangneux ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Agricultural Soil ◽  
Bacterial Abundance ◽  
Rapid Flow

scholarly journals Rapid Flow Cytometry-Based Test for the Diagnosis of Lipopolysaccharide Responsive Beige-Like Anchor (LRBA) Deficiency

2018 ◽  
Vol 9 ◽  
Author(s):  
Laura Gámez-Díaz ◽  
Elena C. Sigmund ◽  
Veronika Reiser ◽  
Werner Vach ◽  
Sophie Jung ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rapid Flow ◽  
Lrba Deficiency

scholarly journals Clinical research assessment by flow cytometry of biomarkers for infectious stratification in an Emergency Department

2019 ◽  
Vol 13 (16) ◽  
pp. 1373-1386 ◽  
Author(s):  
Pénélope Bourgoin ◽  
Thomas Soliveres ◽  
Dalia Ahriz ◽  
Isabelle Arnoux ◽  
Christian Meisel ◽  
...  
Keyword(s):  
Emergency Department ◽  
Flow Cytometry ◽  
Clinical Research ◽  
Bacterial Infections ◽  
Viral Infections ◽  
Point Of Care ◽  
New Combination ◽  
Research Assessment ◽  
Rapid Flow ◽  
Clinical Records

Aim: Management of patients with infections within the Emergency Department (ED) is challenging for practitioners, as the identification of infectious causes remains difficult with current techniques. A new combination of two biomarkers was tested with a new rapid flow cytometry technique. Materials & methods: Subjects from the ED were tested for their CD64 on neutrophils (nCD64) and CD169 on monocytes (mCD169) levels and results were compared to their clinical records. Results: Among 139 patients, 29% had confirmed bacterial infections and 5% viral infections. nCD64 and mCD169 respectively showed 88 and 86% sensitivity and 90 and 100% specificity for identifying subjects in bacterial or viral conditions. Conclusion: This point-of-care technique could allow better management of patients in the ED.

scholarly journals Rapid Flow Cytometry Method for Quantitation of LFA-1-Adhesive T Cells

2006 ◽  
Vol 13 (3) ◽  
pp. 403-408 ◽  
Author(s):  
Brian Crucian ◽  
Mayra Nelman-Gonzalez ◽  
Clarence Sams
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
T Cell Subsets ◽  
Activated T Cells ◽  
Peripheral Blood Mononuclear ◽  
Simple Method ◽  
Rapid Flow

ABSTRACT Adhesion molecules are important for leukocyte endothelial attachment and migration to sites of inflammation. The LFA-1 (CD11a and CD18) integrin molecule is constitutively expressed on the T-cell surface. Following T-cell activation, a rapid conformational change of LFA-1 to an “adhesive” state occurs, allowing LFA-1 binding to intracellular cell adhesion molecule type 1 (ICAM-1)-expressing targets, such as antigen-presenting cells. For this study, a rapid flow cytometry method for the quantitation of LFA-1-adhesive T cells following activation was developed. Purified ICAM-1 was bound to 4.5-μm-diameter beads. Following peripheral blood mononuclear cell activation culture (phorbol myristate acetate and ionomycin), the cells were incubated with the ICAM-1 beads, which allowed attachment to occur. The T cell-bead complexes were then resolved from unbound T cells by flow cytometry. Multicolor analysis allowed a complete phenotypic analysis of the adhesive T-cell subsets. Experimental controls indicated that the T cell-bead attachment was LFA-1 and ICAM-1 specific. Very little binding between unactivated T cells and ICAM beads or between activated T cells and plain beads was observed. The kinetics of the response was extremely rapid, with nearly maximal numbers of adhesive T cells observed following 5 min of activation. Scanning electron microscopy analysis was used to characterize legitimate bead-cell binding. By using multicolor cytometry, the responding adhesive T-cell population was usually identified as a distinct subset of T cells with the following phenotype: CD3+ CD4+ or CD8+ CD19− CD16− CD45RO+ CD62L+ CD27+ CD57−. A rapid and simple method for the scoring of LFA-1-adhesive T cells was developed and may have significant utility for immune function studies.

scholarly journals CD64 and CD169 could help differentiate bacterial from viral infections in Emergency Department

2020 ◽  
Author(s):  
Pénélope Bourgoin ◽  
Thomas Soliveres ◽  
Alexandra Barbaresi ◽  
Anderson Loundou ◽  
Isabelle Arnoux ◽  
...  
Keyword(s):  
Emergency Department ◽  
Flow Cytometry ◽  
Bacterial Infections ◽  
Viral Infections ◽  
Rapid Flow ◽  
Syncytial Virus ◽  
Potential Improvement ◽  
Clinical Records ◽  
Diagnostic Outcomes ◽  
Febrile Syndrome

ABSTRACTBackgroundThe identification of a bacterial, viral or even non-infectious cause is essential in the management of febrile syndrome in the emergency department (ED) setting, especially in epidemic contexts such as flu or CoVID-19.ObjectivesThe aim of this study was to assess discriminative performances of two biomarkers, CD64 on neutrophils (nCD64) and CD169 on monocytes (mCD169), using a new flow cytometry procedure, in patients presenting with fever to the ED. Human leucocyte antigen-DR on monocytes (mHLA-DR), HLA-ABC ratio (rHLA-ABC), and CD64 on monocytes (mCD64) were also assessed.Methods85 adult patients presenting with potential infection were included during the 2019 flu season in the ED of La Timone Hospital. They were divided into four diagnostic outcomes according to their clinical records: no-infection, bacterial infection, viral infection and co-infection.ResultsmCD169 was elevated in patients suffering from Flu A virus or Respiratory Syncytial Virus, while nCD64 was mainly found elevated in subjects with Streptococcus pneumoniae. In total, 38 (45%) patients were diagnosed with bacterial infections, 11 (13%) with viral infections and 29 (34%) with co-infections. nCD64 and mCD169 showed 90% and 80% sensitivity, and 78% and 91% specificity, respectively, for identifying patients with bacterial or viral infections. Other biomarkers had lower discriminative performances.ConclusionsnCD64 and mCD169 have potential for accurately distinguishing bacterial and acute viral infections. Combined in an easy and rapid flow cytometry procedure, they constitute a potential improvement for infection management in the ED setting, and could even help for the triage of patients during emerging epidemics.

scholarly journals Rapid Flow Cytometry of Gastrointestinal Stromal Tumours Closely Matches the Modified Fletcher Classification

2021 ◽  
Vol 41 (1) ◽  
pp. 131-136
Author(s):  
KIYOAKI TANIGUCHI ◽  
AKANE SUZUKI ◽  
AKIKO SERIZAWA ◽  
SHO KOTAKE ◽  
SHUNICHI ITO ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Gastrointestinal Stromal Tumours ◽  
Rapid Flow
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