scholarly journals Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris -(bathophenanthroline disulfonate): The poor man's fluorescent detection method

PROTEOMICS ◽  
2010 ◽  
Vol 11 (2) ◽  
pp. 324-328 ◽  
Author(s):  
Catherine Aude-Garcia ◽  
Véronique Collin-Faure ◽  
Sylvie Luche ◽  
Thierry Rabilloud
Keyword(s):  
Detection Method ◽  
Fluorescent Detection ◽  
The Poor ◽  
Bathophenanthroline Disulfonate

scholarly journals A highly sensitive near-infrared fluorescent detection method to analyze signalling pathways by reverse-phase protein array

PROTEOMICS ◽  
2009 ◽  
Vol 9 (24) ◽  
pp. 5446-5454 ◽  
Author(s):  
Laurence Dupuy ◽  
Christophe Gauthier ◽  
Guillaume Durand ◽  
Astrid Musnier ◽  
Domitille Heitzler ◽  
...  
Keyword(s):  
Near Infrared ◽  
Detection Method ◽  
Protein Array ◽  
Fluorescent Detection ◽  
Signalling Pathways ◽  
Reverse Phase Protein Array ◽  
Reverse Phase ◽  
Highly Sensitive ◽  
Phase Protein

A solvent-dependent fluorescent detection method for Fe3+ and Hg2+ based on a rhodamine B derivative

Talanta ◽  
2016 ◽  
Vol 154 ◽  
pp. 329-334 ◽  
Author(s):  
Xutian Li ◽  
Yue Yin ◽  
Junjie Deng ◽  
Huixian Zhong ◽  
Jian Tang ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Detection Method ◽  
Fluorescent Detection

The Contour Size Detection of Driver Airbag Based on Image Processing Technology

2013 ◽  
Vol 658 ◽  
pp. 546-550
Author(s):  
Xiao Dong Wang ◽  
Xiao Wei Chen ◽  
Wei Zhang ◽  
Bo Liu ◽  
Liang Dong An
Keyword(s):  
Image Processing ◽  
Template Matching ◽  
Detection Method ◽  
Detection Efficiency ◽  
Ccd Camera ◽  
Processing Technology ◽  
Edge Extraction ◽  
Image Preprocessing ◽  
The Poor ◽  
Image Processing Technology

In this paper we have developed a new methodology for detecting the contour size of driver airbag based on image processing technology and Machine vision. Through the CCD camera we can obtain the image, and then do the following operations by a computer, such as binarization, edge extraction and so on the other image preprocessing. This methodology uses intelligent template matching technology to detect the airbags and by comparing with the predefined parameter to determine whether the contour size is qualified .The experimental results show that: this new detection method solves the disadvantage of traditional detection method, such as the low detection efficiency, the detection precision is not high, the poor detection repeatability, the higher rate of detection miscarriage of justice.

scholarly journals Development of a fluorescent immunochromatographic assay based on quantum dots for the detection of fleroxacin

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22005-22013
Author(s):  
Qingbao Yang ◽  
Yanhua Qi ◽  
Jingming Zhou ◽  
Yumei Chen ◽  
Chao Liang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Fluorescent Probe ◽  
Rapid Detection ◽  
Detection Method ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Immunochromatographic Assay

(1) Water-soluble CdSe/ZnS QDs and an anti-FLE monoclonal antibody were used to prepare a fluorescent probe. (2) Primary rapid detection of FLE residues with visual fluorescent detection method.

scholarly journals Detection Method of Computer Room Personnel Based on Improved DERT

2021 ◽  
Author(s):  
Dan Su ◽  
Qiong-lan Na ◽  
Hui-min He ◽  
Yi-xi Yang
Keyword(s):  
Target Detection ◽  
Detection Method ◽  
Poor Performance ◽  
Small Target ◽  
Spatial Query ◽  
Two Stage ◽  
Slow Convergence ◽  
Small Target Detection ◽  
The Poor ◽  
Channel Information

Recently developed methods such as DETR [1] apply Transformer [2] structure to target detection. The performance of using Transformers for target detection (DETR) is similar to that of two-stage target detector. First of all, this paper attempts to apply Transformer to computer room personnel detection. The contributions of the improved DETR include: 1) in order to improve the poor performance of small target detection. Embed Depthwise Convolution in the encoder. When the coding feature is reconstructed, the channel information is retained. 2) in order to solve the problem of slow convergence in DETR training. This paper improves the cross-attention in DECODE and adds the spatial query module. It can accelerate the convergence of DETR. The convergence speed of the improved method is six times faster than that of the original DETR, and the mAP0.5 is improved by 3.1%.

Capillary Electrophoresis With Laser-Induced Fluorescent Detection Method Using Highly Emissive Probes for Analysis of Actinides in Radioactive Wastes

2011 ◽  
Author(s):  
Tomoko Haraga ◽  
Yuta Nakano ◽  
Masami Shibukawa ◽  
Yutaka Kameo ◽  
Kuniaki Takahashi ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Fuel Cycle ◽  
Detection Method ◽  
Radioactive Wastes ◽  
Fluorescent Detection ◽  
Highly Sensitive ◽  
Emissive Probes ◽  
First Time ◽  
Actinide Ions ◽  
Detection And Separation

Actinides are important nuclides for the analysis of radioactive wastes arising from nuclear fuel cycle facilities. In order to achieve simple and rapid analysis of actinides, capillary electrophoresis-laser-induced fluorescent detection method (CE-LIF) is one of the potential candidates. In this study, new emissive probes of actinide ions suitable for CE-LIF were developed for the first time. The detection and separation of americium and neptunium ions as model nuclides were examined using several new emissive complexing probes, each of which possessed a fluorophore and a different chelating moiety. With a pre-capillary complexation technique without addition of the probe to separation buffer electrolyte, the highly sensitive fluorescent detection of Am and Np was successfully achieved using acyclic and macrocyclic multidentate probes. The results suggests that the probe with an acyclic hexadentate chelating moiety is suitable for detection and separation of Am and Np. The detection limit of mid–ppt levels was determined.

Magnetoresistive sensors and magnetic nanoparticles for biotechnology

2005 ◽  
Vol 20 (12) ◽  
pp. 3294-3302 ◽  
Author(s):  
Guenter Reiss ◽  
Hubert Brueckl ◽  
Andreas Huetten ◽  
Joerg Schotter ◽  
Monika Brzeska ◽  
...  
Keyword(s):  
Single Molecule ◽  
Giant Magnetoresistance ◽  
Detection Method ◽  
Automated Analysis ◽  
Fluorescent Detection ◽  
Detection Methods ◽  
Stray Field ◽  
Magnetoresistive Sensor ◽  
Target Molecules ◽  
Magnetic Markers

Magnetoresistive biosensors use a new detection method for molecular recognition reactions based on two recently developed techniques and devices: Magnetic markers and XMR sensors, where XMR means either giant magnetoresistance (GMR) or tunneling magnetoresistance (TMR). The markers are specifically attached to the target molecules, and their magnetic stray field is picked up by an embedded magnetoresistive sensor as a change of the electrical resistance. Compared to established, e.g., fluorescent, detection methods, magnetic biosensors have a number of advantages, including low molecular detection limits, flexibility, and the direct availability of an electronic signal suitable for further automated analysis. This makes them a promising choice for the detection units of future widespread and easy-to-use lab-on-a-chip systems or biochips. In this article, we discuss recent advances in this field and compare possible approaches toward single molecule detection.

scholarly journals THE DEVELOPMENT OF TEST SYSTEMS FOR DETERMINING THE EXPRESSION OF THE BIRC5 AND HER-2/NEU GENES IN LABORATORY ANIMALS USING PCR WITH FLUORESCENCE DETECTION AND EVALUATION OF THEIR EFFECTIVENESS

2021 ◽  
Vol 20 (2) ◽  
pp. 65-70
Author(s):  
V.M. Semenov ◽  
◽  
V.V. Pabiarzhyn ◽  
E.S. Pashinskaya ◽  
S.K. Egorov ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Detection Method ◽  
Test System ◽  
Fluorescent Detection ◽  
Laboratory Animals ◽  
Test Systems ◽  
Highly Sensitive ◽  
Her 2 ◽  
The Given ◽  
Pcr Test

Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method. Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment. Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective. Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.

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