scholarly journals Development of a fluorescent immunochromatographic assay based on quantum dots for the detection of fleroxacin

RSC Advances ◽  
2021 ◽  
Vol 11 (36) ◽  
pp. 22005-22013
Author(s):  
Qingbao Yang ◽  
Yanhua Qi ◽  
Jingming Zhou ◽  
Yumei Chen ◽  
Chao Liang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Fluorescent Probe ◽  
Rapid Detection ◽  
Detection Method ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Immunochromatographic Assay

(1) Water-soluble CdSe/ZnS QDs and an anti-FLE monoclonal antibody were used to prepare a fluorescent probe. (2) Primary rapid detection of FLE residues with visual fluorescent detection method.

Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
qianqian lu ◽  
Xin-Xin Xu ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum ◽  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Immunochromatographic Strip

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and...

scholarly journals Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies

2012 ◽  
Vol 2 (1) ◽  
pp. 5 ◽  
Author(s):  
Fabio Cimaglia ◽  
Alessandro Aliverti ◽  
Maurizio Chiesa ◽  
Palmiro Poltronieri ◽  
Enrico De Lorenzis ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantum Dots ◽  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Detection System ◽  
Lateral Flow ◽  
Fluorescence Signal ◽  
Human Pathogens ◽  
Detection Zone ◽  
Diagnostic Investigation

A lateral flow (LF) device combined with quantum dots (QDs) technology was developed for rapid detection of a specific mycobacterial flavoprotein reductase (<em>fprA</em>). In order to develop the LF assay based on a double-antibody sandwich format, two monoclonal antibodies recognizing different epitopes located in separated <em>fprA</em> domains were identified. The first monoclonal antibody was immobilized onto the detection zone of a porous nitrocellulose membrane, whereas another monoclonal antibody was conjugated to QDs nanoparticles as a detection system. Using these monoclonal antibodies we recorded a good fluorescence signal, the intensity of which was directly proportional to the concentration of <em>fprA</em> protein. The use of antibodies conjugated with fluorescent semiconductor QDs via biotin-streptavidin bridge, allowed the detection of <em>fprA</em> protein at concentrations as low as 12.5 pg/μL in less than 10 min. The reported technology could be useful in the diagnostic investigation of <em>Mycobacterium tuberculosis</em> and other human pathogens in clinical specimens.

Water-soluble MoS2 quantum dots are a viable fluorescent probe for hypochlorite

2018 ◽  
Vol 185 (4) ◽  
Author(s):  
Yi Wang ◽  
Pu Zhang ◽  
Qing Lu ◽  
Yue Wang ◽  
Wensheng Fu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescent Probe ◽  
Water Soluble

scholarly journals Waveguide-Based Fluorescent Immunosensor for the Simultaneous Detection of Carbofuran and 3-Hydroxy-Carbofuran

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 191
Author(s):  
Weiming Sun ◽  
Lanhua Liu ◽  
Abdul Ghaffar Memon ◽  
Xiaohong Zhou ◽  
Hongwei Zhao
Keyword(s):  
Water Quality ◽  
Monoclonal Antibody ◽  
Human Health ◽  
Broad Spectrum ◽  
Rapid Detection ◽  
Detection Method ◽  
Simultaneous Detection ◽  
Agricultural Products ◽  
Linear Detection ◽  
High Binding Affinity

Carbofuran (CBF) is an efficient and broad-spectrum insecticide. As testing indicators for water quality and agricultural products, CBF and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are regulated by many countries. The detection of CBF and 3-OH-CBF is of great importance for the environment and human health. However, an immunosensor detection method for the simultaneous analysis of CBF and 3-OH-CBF remains unavailable. Herein, we report a waveguide-based fluorescent immunosensor for detecting CBF and 3-OH-CBF, synchronously. The immunosensor is based on a broad-spectrum monoclonal antibody with high binding affinity against CBF and 3-OH-CBF. The linear detection ranges for CBF and 3-OH-CBF are 0.29–2.69 and 0.12–4.59 μg/L, with limits of detection of 0.13 μg/L for CBF and 0.06 μg/L for 3-OH-CBF, respectively. The whole detection process for each cycle is approximately 30 min. The results show a good application prospect for the rapid detection of CBF and 3-OH-CBF in water or agricultural products.

Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe

2018 ◽  
Vol 185 (11) ◽  
Author(s):  
Fangmei Zhang ◽  
Pinyi Ma ◽  
Xinyu Deng ◽  
Ying Sun ◽  
Xinghua Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Uric Acid ◽  
Fluorescent Probe ◽  
Water Soluble ◽  
Enzymatic Determination

A sensitivity-enhanced heterologous immunochromatographic assay based on a monoclonal antibody for the rapid detection of histamine in saury samples

RSC Advances ◽  
2015 ◽  
Vol 5 (96) ◽  
pp. 78833-78840 ◽  
Author(s):  
Lin Luo ◽  
Jin-Yi Yang ◽  
Zhi-Li Xiao ◽  
Dao-Ping Zeng ◽  
Yong-Jun Li ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rapid Detection ◽  
Immunochromatographic Assay

An immunochromatograhic strip for rapid detection of histamine based on a sensitive monoclonal antibody against p-nitrobenzoylated histamine was firstly reported.

The selective deprotonation of carbon quantum dots for fluorescent detection of phosphate and visualization of latent fingerprints

Nanoscale ◽  
2021 ◽  
Author(s):  
Li Yang ◽  
Qi Zhang ◽  
Ying Han ◽  
Hongjuan Li ◽  
Shiguo Sun ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Rapid Determination ◽  
Carbon Quantum Dots ◽  
Water Soluble ◽  
Fluorescent Detection ◽  
Latent Fingerprints ◽  
Fluorescent Sensing ◽  
Turn On ◽  
Sensing Platform

We developed a water-soluble, stable and selective “turn-on” fluorescent sensing platform based on carbon quantum dots (CQDs) for rapid determination of the phosphate (Pi) in aqueous solutions and visualization of...

Colorimetric and near infrared fluorescent detection of cyanide by a new phenanthroimidazole–indolium conjugated probe

RSC Advances ◽  
2014 ◽  
Vol 4 (28) ◽  
pp. 14752-14757 ◽  
Author(s):  
Yi Zhang ◽  
Dehuan Yu ◽  
Guoqiang Feng
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Near Infrared ◽  
High Selectivity ◽  
Fluorescent Detection

A new dual colorimetric and fluorescent probe was developed for rapid detection of cyanide anions with high selectivity and sensitivity.

Synthesis of novel quantum dots from DNA under mild conditions and their use as a fluorescent probe for the detection of Fe3+ and in biological imaging

2015 ◽  
Vol 7 (15) ◽  
pp. 6274-6279 ◽  
Author(s):  
Guifang Cheng ◽  
Weibo Zhang ◽  
Ying Zhou ◽  
Qifeng Ge ◽  
Chaobiao Huang
Keyword(s):  
Aqueous Solution ◽  
Quantum Dots ◽  
Fluorescent Probe ◽  
Biological Imaging ◽  
Water Soluble ◽  
Iron Ions ◽  
Simple Method ◽  
Mild Conditions

We have demonstrated a simple method for the synthesis of water-soluble and well-dispersed fluorescent DNA-dots under mild conditions, which could be used as an effective fluorescent probe for the detection of iron ions with relatively good selectivity and sensitivity in an aqueous solution as well as in biological imaging applications.

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