Capillary Electrophoresis With Laser-Induced Fluorescent Detection Method Using Highly Emissive Probes for Analysis of Actinides in Radioactive Wastes

2011 ◽  
Author(s):  
Tomoko Haraga ◽  
Yuta Nakano ◽  
Masami Shibukawa ◽  
Yutaka Kameo ◽  
Kuniaki Takahashi ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Fuel Cycle ◽  
Detection Method ◽  
Radioactive Wastes ◽  
Fluorescent Detection ◽  
Highly Sensitive ◽  
Emissive Probes ◽  
First Time ◽  
Actinide Ions ◽  
Detection And Separation

Actinides are important nuclides for the analysis of radioactive wastes arising from nuclear fuel cycle facilities. In order to achieve simple and rapid analysis of actinides, capillary electrophoresis-laser-induced fluorescent detection method (CE-LIF) is one of the potential candidates. In this study, new emissive probes of actinide ions suitable for CE-LIF were developed for the first time. The detection and separation of americium and neptunium ions as model nuclides were examined using several new emissive complexing probes, each of which possessed a fluorophore and a different chelating moiety. With a pre-capillary complexation technique without addition of the probe to separation buffer electrolyte, the highly sensitive fluorescent detection of Am and Np was successfully achieved using acyclic and macrocyclic multidentate probes. The results suggests that the probe with an acyclic hexadentate chelating moiety is suitable for detection and separation of Am and Np. The detection limit of mid–ppt levels was determined.

scholarly journals Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 95 ◽  
Author(s):  
Shuangjiao Xu ◽  
Kehai Zhou ◽  
Dan Fang ◽  
Lei Ma
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Hplc Method ◽  
Fluorescent Detection ◽  
Copper Nanoclusters ◽  
Highly Sensitive ◽  
The Us ◽  
Fluorescence Quenching Mechanism ◽  
First Time

In this paper, fluorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the first time. Based on a fluorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, fluorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1–100 µM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 µM) defined by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.

scholarly journals A highly sensitive near-infrared fluorescent detection method to analyze signalling pathways by reverse-phase protein array

PROTEOMICS ◽  
2009 ◽  
Vol 9 (24) ◽  
pp. 5446-5454 ◽  
Author(s):  
Laurence Dupuy ◽  
Christophe Gauthier ◽  
Guillaume Durand ◽  
Astrid Musnier ◽  
Domitille Heitzler ◽  
...  
Keyword(s):  
Near Infrared ◽  
Detection Method ◽  
Protein Array ◽  
Fluorescent Detection ◽  
Signalling Pathways ◽  
Reverse Phase Protein Array ◽  
Reverse Phase ◽  
Highly Sensitive ◽  
Phase Protein

scholarly journals THE DEVELOPMENT OF TEST SYSTEMS FOR DETERMINING THE EXPRESSION OF THE BIRC5 AND HER-2/NEU GENES IN LABORATORY ANIMALS USING PCR WITH FLUORESCENCE DETECTION AND EVALUATION OF THEIR EFFECTIVENESS

2021 ◽  
Vol 20 (2) ◽  
pp. 65-70
Author(s):  
V.M. Semenov ◽  
◽  
V.V. Pabiarzhyn ◽  
E.S. Pashinskaya ◽  
S.K. Egorov ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Detection Method ◽  
Test System ◽  
Fluorescent Detection ◽  
Laboratory Animals ◽  
Test Systems ◽  
Highly Sensitive ◽  
Her 2 ◽  
The Given ◽  
Pcr Test

Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method. Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment. Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective. Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.

scholarly journals Anchoring zinc-doped carbon dots on a paper-based chip for highly sensitive fluorescence detection of copper ions

The Analyst ◽  
2021 ◽  
Vol 146 (20) ◽  
pp. 6297-6305
Author(s):  
Qinglan Miao ◽  
Ji Qi ◽  
Yuanyuan Li ◽  
Xinxia Fan ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Carbon Dots ◽  
Active Sites ◽  
Copper Ions ◽  
Fluorescent Detection ◽  
Zn Doping ◽  
Highly Sensitive ◽  
Doped Carbon Dots

A novel paper-based chip that anchored zinc-doped carbon dots was constructed for sensitive and stable fluorescent detection of Cu2+. Zn doping increased the active sites for simplifying the modification of carbon dots.

scholarly journals Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1207
Author(s):  
Hong Jae Cheon ◽  
Quynh Huong Nguyen ◽  
Moon Il Kim
Keyword(s):  
Magnetic Nanoparticles ◽  
High Sensitivity ◽  
High Specificity ◽  
Choline Oxidase ◽  
Fluorescent Detection ◽  
One Pot ◽  
Site Structure ◽  
Specificity And Sensitivity ◽  
Highly Sensitive ◽  
Peroxidase Mimics

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.

Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection

2006 ◽  
Vol 1113 (1-2) ◽  
pp. 251-254 ◽  
Author(s):  
Xiangyi Huang ◽  
Jifang Weng ◽  
Fuming Sang ◽  
Xingtao Song ◽  
Chengxi Cao ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantum Dot ◽  
Fluorescent Detection

Highly sensitive detection method of retinoblastoma genetic predisposition and biomarkers

2021 ◽  
Author(s):  
Jessica Le Gall ◽  
Catherine Dehainault ◽  
Camille Benoist ◽  
Alexandre Matet ◽  
Livia Lumbroso-Le Rouic ◽  
...  
Keyword(s):  
Genetic Predisposition ◽  
Detection Method ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection
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