Fractionation of Polymeric Procyanidins from Lowbush Blueberry and Quantification of Procyanidins in Selected Foods with an Optimized Normal-Phase HPLC−MS Fluorescent Detection Method

2002 ◽  
Vol 50 (17) ◽  
pp. 4852-4860 ◽  
Author(s):  
Liwei Gu ◽  
Mark Kelm ◽  
John F. Hammerstone ◽  
Gary Beecher ◽  
David Cunningham ◽  
...  
Keyword(s):  
Detection Method ◽  
Normal Phase ◽  
Fluorescent Detection ◽  
Lowbush Blueberry ◽  
Normal Phase Hplc

Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Landreau ◽  
S Bertrand ◽  
C Simoes-Pires ◽  
L Marcourt ◽  
TD Bach ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Acetylcholinesterase Activity ◽  
Normal Phase ◽  
Normal Phase Hplc

Enantioseparation of novel chiral tetrahedral clusters on an amylose tris-(3,5-dimethylphenylcarbamate) chiral stationary phase by normal phase HPLC

2010 ◽  
Vol 22 (10) ◽  
pp. 1070-1074 ◽  
Author(s):  
Wen-Zhi Li ◽  
Xia Wang ◽  
Wei-Qiang Zhang ◽  
Chen Li-Ren ◽  
Yong-Min Li ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Chiral Stationary Phase ◽  
Normal Phase ◽  
Normal Phase Hplc

Diacylglycerols interfere in normal phase HPLC analysis of lipoxygenase products of docosahexaenoic or arachidonic acids

1986 ◽  
Vol 32 (6) ◽  
pp. 813-827 ◽  
Author(s):  
Magda Claeys ◽  
Haydee E.P. Bazan ◽  
Dale L. Birkle ◽  
Nicolas G. Bazan
Keyword(s):  
Hplc Analysis ◽  
Normal Phase ◽  
Lipoxygenase Products ◽  
Normal Phase Hplc ◽  
Arachidonic Acids

Extraction, Cleanup, and Quantitative Determination of Aflatoxins in Corn

1980 ◽  
Vol 63 (3) ◽  
pp. 631-633 ◽  
Author(s):  
James E Thean ◽  
David R Lorenz ◽  
David M Wilson ◽  
Kathleen Rodgers ◽  
Richard C Gueldner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Ammonium Sulfate ◽  
Silica Gel ◽  
Normal Phase ◽  
Aqueous Methanol ◽  
Normal Phase Hplc ◽  
Water Saturated

Abstract A method is proposed for extraction and cleanup of corn samples for the quantitation of 4 aflatoxins by high pressure liquid chromatography (HPLC). After aqueous methanol extraction, ammonium sulfate treatment, and partition of aflatoxins into chloroform, sample extracts are partially purified on Sep-Pak cartridges or small columns packed with HPLC grade silica; cleanup requires only 13 mL solvent/sample. Aflatoxins B1, B2, G1, and G2 in the purified extract are resolved in ca 10 min by normal phase HPLC on a microparticulate (5 μm) silica gel column with a 50% water-saturated chloroform-cyclohexaneacetonitrile- ethanol solvent, and are measured by ultraviolet fluorescence in a silica gel-packed flowcell. Recoveries of added aflatoxins B1, B2, G1, and G2 were 84–118 % at levels of 1.5–125 μg/kg

Simultaneous determination of endogenous retinoic acid isomers and retinol in human plasma by isocratic normal-phase HPLC with ultraviolet detection

1994 ◽  
Vol 40 (1) ◽  
pp. 48-51 ◽  
Author(s):  
E Meyer ◽  
W E Lambert ◽  
A P De Leenheer
Keyword(s):  
Retinoic Acid ◽  
Human Plasma ◽  
Simple Procedure ◽  
Internal Standard ◽  
High Sensitivity ◽  
Normal Phase ◽  
Ultraviolet Detection ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Normal Phase Hplc

Abstract We describe a rapid and simple procedure for the simultaneous quantitation of endogenous 13-cis-retinoic acid, all-trans-retinoic acid, and retinol by isocratic normal-phase HPLC with ultraviolet detection, in 0.5 mL of human plasma. A silica adsorption column was eluted with n-hexane:2-propanol:acetic acid (200:0.7:0.135 by vol) at 0.9 mL/min, and the effluent monitored at 350 nm. The arotinoid ethylsulfonic acid Ro 15-1570 was used as the internal standard. High sensitivity, allowing quantitation of physiological concentrations, was achieved, particularly for the retinoic acid isomers. The detection limits were 0.5 microgram/L in plasma for both 13-cis- and trans-retinoic acid, and 10 micrograms/L for retinol. The CVs for between-day determinations of the lowest quality-control concentration (n = 12) were 4.8% for 13-cis-retinoic acid, 3.4% for trans-retinoic acid, and 3.0% for retinol. The mean (+/- SD) concentrations of 13-cis-retinoic acid (1.79 +/- 0.56 microgram/L), trans-retinoic acid (1.35 +/- 0.42 microgram/L), and retinol (533 +/- 58 micrograms/L) measured in plasma from 22 healthy volunteers agreed well with those previously reported.

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