Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase

1990 ◽  
Vol 162 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Andrew F. Walls ◽  
David B. Jones ◽  
Julie H. Williams ◽  
Martin K. Church ◽  
Stephen T. Holgate
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Fixed Tissue ◽  
Immunohistochemical Identification

Generation of anti-DC-SIGN monoclonal antibodies capable of blocking HIV-1 gp120 binding and reactive on formalin-fixed tissue

2011 ◽  
Vol 135 (1-2) ◽  
pp. 165-172 ◽  
Author(s):  
Bertrand Canard ◽  
Hortense Vachon ◽  
Thomas Fontaine ◽  
Jean-Jacques Pin ◽  
Stéphane Paul ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Gp120 Binding ◽  
Hiv 1 ◽  
Formalin Fixed

Monoclonal antibodies against rat mast cells differentiate between subtypes

1987 ◽  
Vol 20 (3-4) ◽  
pp. 216-218 ◽  
Author(s):  
H. Repke ◽  
N. Rossow ◽  
S. Savoly ◽  
J. Odarjuk ◽  
L. Karawajew ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Rat Mast Cells

Kinetics of Fc.epsilon.RI Dimer Formation by Specific Monoclonal Antibodies on Mast Cells

Biochemistry ◽  
1994 ◽  
Vol 33 (29) ◽  
pp. 8813-8825 ◽  
Author(s):  
Reinhard Schweitzer-Stenner ◽  
Enrique Ortega ◽  
Israel Pecht
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Dimer Formation ◽  
Kinetics Of

scholarly journals Immunohistochemical identification of mast cells in paraffin- and epon-embedded tissues using platelet factor 41.

1982 ◽  
Vol 30 (2) ◽  
pp. 185-188 ◽  
Author(s):  
S T Shaw ◽  
P C Roche ◽  
G Schmer ◽  
L K Macaulay
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Human Platelet ◽  
Rabbit Antiserum ◽  
Platelet Factor 4 ◽  
Immunoperoxidase Staining ◽  
Immunoperoxidase Method ◽  
Platelet Factor ◽  
Immunohistochemical Identification

An immunoperoxidase method has been developed for staining heparin in the granules of mast cells. The method employs human platelet factor 4 (or anti-heparin) and a rabbit antiserum to this polypeptide. Platelet factor 4 binds to mast cell heparin and provides the basis for immunoperoxidase staining using the rabbit antiserum. Preliminary studies of mast cells in various tissues indicate that the stain is quite specific for the content of mast cell granules, presumably heparin and possibly other glycosaminoglycans.

Production of monoclonal antibodies to lactate dehydrogenase (LDH) isoenzymes for immunohistochemical study on fixed tissue section

1989 ◽  
Vol 21 (11) ◽  
pp. 638-644 ◽  
Author(s):  
Langxing Pan ◽  
Peter C. L. Beverley ◽  
Lynda G. Bobrow ◽  
Dallas M. Swallow ◽  
Peter G. Isaacson
Keyword(s):  
Monoclonal Antibodies ◽  
Lactate Dehydrogenase ◽  
Tissue Section ◽  
Immunohistochemical Study ◽  
Fixed Tissue ◽  
Ldh Isoenzymes

scholarly journals Production of Specific Monoclonal Antibodies toAspergillus Species and Their Use in Immunohistochemical Identification of Aspergillosis

1999 ◽  
Vol 37 (4) ◽  
pp. 1221-1223 ◽  
Author(s):  
L. E. Fenelon ◽  
A. J. Hamilton ◽  
J. I. Figueroa ◽  
M. A. Bartholomew ◽  
M. H. Allen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoperoxidase Staining ◽  
Frozen Sections ◽  
Clinical Specimens ◽  
Murine Monoclonal Antibodies ◽  
Immunohistochemical Identification

Two anti-Aspergillus murine monoclonal antibodies (MAbs), designated 164G and 611F, have been produced; both specifically recognize cytoplasmic antigens of A. fumigatus, A. flavus, and A. niger by enzyme-linked immunosorbent assay. The MAbs can identify Aspergillus spp. both in frozen sections by immunofluorescence and in paraffin-embedded clinical specimens by immunofluorescence and immunoperoxidase staining.

Do Lipocalins Play a Role in Mast Cell Physiology?.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1645-1645
Author(s):  
Lennart E. Logdberg ◽  
Bo Akerstrom ◽  
Gregory A. Hair ◽  
Maria Allhorn ◽  
Tatyana Vikulina ◽  
...  
Keyword(s):  
Mast Cell ◽  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Cell Surface ◽  
Gene Transcription ◽  
Cell Surface Receptor ◽  
Cell Surface Expression ◽  
Cell Physiology ◽  
Myeloma Protein ◽  
Surface Receptor

Abstract Introduction. Aiming to identify molecular properties of allergens responsible for their “intrinsic allergenicity”, we focus on a subset of xenogeneic lipocalins (LCs) that comprise the major mammalian respiratory allergens. These structurally and functionally homologous molecules likely possess conserved molecular motifs promoting IgE-dependent allergy. We hypothesize that such LC “allergenicity” depends on non-IgE interactions of LCs with components of the innate immune system. Herein we describe a possible basis for such interactions between LCs and mast cells. Materials and Methods. Two sources of human mast cells were used; primary cultures derived from peripheral blood CD34+ progenitor cells; or the LAD-2 cell line. Cells were cultured in serum-free medium with recombinant human stem cell factor (SCF; 100 ng/ml). Monoclonal antibodies to human gp330/megalin (MAb E11) were a kind gift of Prof. Lars Rask (Uppsala University, Sweden). Cell surface protein expression was assessed by flow cytometry and gene transcription was measured by real-time PCR. Results. Monoclonal antibodies to an endocytic cell surface receptor (megalin, also known as low-density lipoprotein receptor-related protein (LRP)-2) known to bind multiple LCs stained the mast cell lines. This putative expression of megalin by the mast cells corresponded to their transcription of megalin mRNA as shown as by PCR. Moreover, mast cell megalin gene transcription could be induced (≥ 1000-fold) by overnight culture with monomeric IgE myeloma protein (100 ng/ml), and such induction of the megalin message correlated with both an increase in cell surface expression of the molecule and the specific binding of a purified human LC (a-1 microglobulin). Conclusion. Megalin, an LC-binding cell surface receptor, appears to be constitutively expressed by both progenitor and mature mast cells, and its expression seems to be strongly upregulated by culture with monomeric IgE. This is consistent with a role for direct mast cell-LC interactions in the development of IgE-dependent allergy. In addition, and also of potential clinical relevance, endogenous LCs may play a functional role in normal mast cell physiology.

Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells

1986 ◽  
Vol 17 (5-6) ◽  
pp. 418-426 ◽  
Author(s):  
G. Micklefield ◽  
W. König ◽  
Ph. Pfeiffer ◽  
A. Bohn
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Ige Receptor ◽  
Rbl Cells ◽  
Basophilic Leukemia ◽  
Rat Mast Cells
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