Production of monoclonal antibodies to lactate dehydrogenase (LDH) isoenzymes for immunohistochemical study on fixed tissue section

1989 ◽  
Vol 21 (11) ◽  
pp. 638-644 ◽  
Author(s):  
Langxing Pan ◽  
Peter C. L. Beverley ◽  
Lynda G. Bobrow ◽  
Dallas M. Swallow ◽  
Peter G. Isaacson
Keyword(s):  
Monoclonal Antibodies ◽  
Lactate Dehydrogenase ◽  
Tissue Section ◽  
Immunohistochemical Study ◽  
Fixed Tissue ◽  
Ldh Isoenzymes

Total lactate dehydrogenase and its isoenzymes in serum in the presence of penicillamine and other sulfhydryl compounds.

1977 ◽  
Vol 23 (2) ◽  
pp. 229-233 ◽  
Author(s):  
L L Gershbein ◽  
K G Raikoff
Keyword(s):  
Lactate Dehydrogenase ◽  
Disc Electrophoresis ◽  
The Other ◽  
Rat Serum ◽  
Initial Concentration ◽  
Ldh Isoenzymes ◽  
Allosteric Effectors ◽  
Sulfhydryl Compounds

Abstract Toward delineation of changes in total lactate dehydrogenase (LDH) and in the distribution of LDH isoenzymes as assessed by polyacrylamide disc electrophoresis, we inbucated human and rat sera with various agents, notably sulfhydryl compounds. Although artefacts were apparent when these agents were used without preliminary adjustment of pH, we saw little alteration in total unitage when one or two volumes of serum was mixed with one volume of any of several thiols, especially penicillamine, at an initial concentration of 0.4 mol/liter and pH 7.0-7.5. Under these conditions, penicillamine caused a loss in LDH-5 after incubation for 1 h at 25 degrees C together with small decreases in mobility of the other four isoenzymes toward the anode. A zymosan region appeared below the albumin and tracking dye area. With longer periods of incubation of rat serum with penicillamine or alpha-mercaptosuccinate, a novel band in the zymogram was noted just above the LDH-4 peak. The observations are discussed in terms of allosteric effectors.

scholarly journals Optimization of immunohistochemical techniques to detect extracellular matrix proteins in fixed skin specimens.

1989 ◽  
Vol 37 (1) ◽  
pp. 105-113 ◽  
Author(s):  
J M Folkvord ◽  
D Viders ◽  
A Coleman-Smith ◽  
R A Clark
Keyword(s):  
Extracellular Matrix ◽  
Tissue Section ◽  
Antigen Expression ◽  
Treatment Technique ◽  
Tissue Architecture ◽  
Fixed Tissue ◽  
Total Recovery ◽  
Salt Extraction ◽  
Immunohistochemical Techniques ◽  
Formalin Fixed

Complete antigen visualization in the context of well-preserved tissue architecture is the goal of all immunohistochemical techniques. Frozen tissue section techniques achieve optimal antigen visualization but preserve tissue architecture poorly. On the other hand, formalin-fixed tissue section techniques preserve tissue architecture very well but result in antigen masking. Enzymatic digestion or salt extraction of formalin-fixed sections has been used to reestablish antigen expression. Recently acid-alcohol-fixed tissue has been used as a successful compromise between tissue architecture preservation and the visualization of cytoskeletal antigens. In an attempt to find an improved immunohistochemical process for non-cytoskeletal antigens, we compared avidin-biotin immunofluorescence staining in frozen, formalin-fixed, and acid-alcohol-fixed tissues. The fixed tissues were either untreated or treated with enzyme digestion or salt extraction. For this study, we examined healing cutaneous wounds in Yorkshire pigs with antibodies to fibronectin, laminin, von Willebrand factor VIII, and keratin. Although tissue architecture was poor, frozen sections provided the best antigen visualization and were therefore used as the standard for complete antigen expression. Formalin-fixed tissues had excellent tissue architecture, but most antigens were completely masked. Pre-treatment technique only partially overcame the antigen masking caused by formalin. In contrast, acid-alcohol fixation preserved tissue architecture almost as well as formalin and sometimes allowed complete antigen visualization; however, laminin and fibronectin were partially masked. Total recovery of the expression of these antigens could be obtained by pre-treating the acid-alcohol-fixed tissue with either hyaluronidase or 1 M NaCl. Therefore, acid-alcohol-fixed tissue appears best for extracellular matrix (ECM) protein immunostaining as well as for cytoskeletal staining. However, certain ECM antigens require hyaluronidase or 1 M NaCl treatment for optimal visualization.

Generation of anti-DC-SIGN monoclonal antibodies capable of blocking HIV-1 gp120 binding and reactive on formalin-fixed tissue

2011 ◽  
Vol 135 (1-2) ◽  
pp. 165-172 ◽  
Author(s):  
Bertrand Canard ◽  
Hortense Vachon ◽  
Thomas Fontaine ◽  
Jean-Jacques Pin ◽  
Stéphane Paul ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Gp120 Binding ◽  
Hiv 1 ◽  
Formalin Fixed

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

2012 ◽  
Vol 111 (4) ◽  
pp. 1645-1650 ◽  
Author(s):  
Jong-Hyun Kim ◽  
Jinyoung Lee ◽  
Hae-Jin Sohn ◽  
Hyun-Ok Song ◽  
Jung-Yeon Kim ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Sandwich Elisa

Serum lactate dehydrogenase (S-LDH) and S-LDH isoenzymes in patients with testicular germ cell tumors

1983 ◽  
Vol 189 (2) ◽  
pp. 326-333 ◽  
Author(s):  
Finn E. von Eyben ◽  
Gunnar Skude ◽  
Sophie D. Fosså ◽  
Olbjørn Klepp ◽  
Ole Børmer
Keyword(s):  
Lactate Dehydrogenase ◽  
Germ Cell ◽  
Germ Cell Tumors ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell ◽  
Ldh Isoenzymes
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