Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells

1986 ◽  
Vol 17 (5-6) ◽  
pp. 418-426 ◽  
Author(s):  
G. Micklefield ◽  
W. König ◽  
Ph. Pfeiffer ◽  
A. Bohn
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Ige Receptor ◽  
Rbl Cells ◽  
Basophilic Leukemia ◽  
Rat Mast Cells

Monoclonal antibodies against rat mast cells differentiate between subtypes

1987 ◽  
Vol 20 (3-4) ◽  
pp. 216-218 ◽  
Author(s):  
H. Repke ◽  
N. Rossow ◽  
S. Savoly ◽  
J. Odarjuk ◽  
L. Karawajew ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Rat Mast Cells

scholarly journals Increased degranulation and phospholipase A2, C, and D activity in RBL cells stimulated through FcepsilonR1 is due to spreading and not simply adhesion

1997 ◽  
Vol 110 (6) ◽  
pp. 771-780 ◽  
Author(s):  
J.R. Apgar
Keyword(s):  
Inositol Phosphate ◽  
Class I ◽  
Leukemia Cells ◽  
Prostaglandin D2 ◽  
Dependent Manner ◽  
Ige Receptor ◽  
Eicosanoid Production ◽  
Rat Basophilic Leukemia Cells ◽  
Rbl Cells ◽  
Basophilic Leukemia

Rat basophilic leukemia cells will adhere to and spread out on fibronectin coated surfaces in an integrin dependent manner. Adhesion and spreading on fibronectin leads to increased degranulation, inositol phosphate production, phospholipase D activation, and increased production of prostaglandin D2 and leukotriene C4 when the cells are activated through the high affinity IgE receptor. Rat basophilic leukemia cells will also adhere to surfaces coated with anti-rat class I antibodies, poly-L-lysine, and a lectin purified from Tetragonolobus purpureas. In all cases, antigen activated cells, which were adherent, displayed increased signaling, degranulation and eicosanoid production as compared to cells which were non-adherent. Cells which adhere to either anti-rat class I antibodies or poly-L-lysine also spread even though this is not mediated through integrins. In contrast, adhesion to the lectin from Tetragonolobus did not cause any appreciable spreading unless the cells were also triggered through the IgE receptor. Cells were also able to bind to fibronectin immobilized on polystyrene beads which mimics adhesion but does not allow spreading. However, these cells exhibited no increased signaling, degranulation, or eicosanoid production. Furthermore, rat basophilic leukemia cells can be modified by incubating them in the presence of biotinylated-phosphatidylserine which becomes incorporated into the membrane. These modified cells will adhere to streptavidin coated plates while unmodified cells will not. However, these modified cells do not spread, even after activation with antigen, and they show no increased degranulation or production of eicosanoids. These results indicate that adhesion itself is not sufficient for upregulation of the cells in response to antigen and that spreading of the cells may be the critical component.

scholarly journals Association of the crosslinked IgE receptor with the membrane skeleton is independent of the known signaling mechanisms in rat basophilic leukemia cells.

1991 ◽  
Vol 2 (3) ◽  
pp. 181-191 ◽  
Author(s):  
J R Apgar
Keyword(s):  
Plasma Membranes ◽  
Second Messengers ◽  
Membrane Skeleton ◽  
Arachidonic Acid Metabolism ◽  
Receptor Interaction ◽  
Critical Event ◽  
Ige Receptor ◽  
Signaling Mechanisms ◽  
Rbl Cells ◽  
Basophilic Leukemia

Crosslinking of the IgE receptor on the surface of rat basophilic leukemia (RBL) cells by multivalent antigen induces an association of these receptors with the detergent-insoluble membrane skeleton. Detergent insolubility of the receptor can also be induced on purified plasma membranes isolated from RBL cells by the use of either IgE oligomers or IgE monomers plus multivalent antigen. The critical event in initiating this interaction between the receptor and the membrane skeleton is cross-linking of the receptor. This association is rapid, and, when triggered by multivalent antigen, it is quickly reversed by the addition of excess monovalent antigen. The fact that this association occurs with the use of purified plasma membranes indicates that all of the components necessary for this interaction are present in the plasma membrane and that intracellular components are not required. Although crosslinking of the receptor activates phospholipase C and phospholipase A2 leading to the generation of several second messengers, none of these signaling mechanisms appears to be involved in IgE receptor interaction with the membrane skeleton. This interaction cannot be induced by phorbol 12-myristate 13-acetate (PMA), ionomycin, or a combination of these two reagents, although this will result in degranulation. Furthermore, receptor detergent insolubility is temperature independent when triggered by multivalent antigen, thus indicating that enzyme-catalyzed reactions are not important. This was verified by the fact that a variety of inhibitors that block phosphatidylinositol metabolism, arachidonic acid metabolism, Ca2+ influx, and protein kinase C (PKC) activation had no effect on antigen-induced association of the receptor with the membrane skeleton. These results indicate that the signaling mechanisms leading to the degranulation response are not involved in the association of the crosslinked receptor with the membrane skeleton.

scholarly journals IgE-receptor activated chloride uptake in relation to histamine secretion from rat mast cells

1994 ◽  
Vol 111 (4) ◽  
pp. 1179-1183 ◽  
Author(s):  
U.G. Friis ◽  
T. Johansen ◽  
N.A. Hayes ◽  
J.C. Foreman
Keyword(s):  
Mast Cells ◽  
Ige Receptor ◽  
Histamine Secretion ◽  
Chloride Uptake ◽  
Rat Mast Cells

Relationship between histocompatibility antigens, other surface determinants and the IgE receptor on rat mast cells

1979 ◽  
Vol 9 (3) ◽  
pp. 219-224 ◽  
Author(s):  
Horst Mossmann ◽  
Balthasar Schmitz ◽  
Wolfgang Lauer ◽  
Uta Staufier ◽  
Marina Gehrung ◽  
...  
Keyword(s):  
Mast Cells ◽  
Ige Receptor ◽  
Histocompatibility Antigens ◽  
Rat Mast Cells

scholarly journals Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells.

1983 ◽  
Vol 80 (10) ◽  
pp. 3050-3053 ◽  
Author(s):  
B. L. Hempstead ◽  
C. W. Parker ◽  
A. Kulczycki
Keyword(s):  
Mast Cells ◽  
Ige Receptor ◽  
Rat Mast Cells
Sign in / Sign up

Export Citation Format

Share Document