Kinetics of Fc.epsilon.RI Dimer Formation by Specific Monoclonal Antibodies on Mast Cells

Biochemistry ◽  
1994 ◽  
Vol 33 (29) ◽  
pp. 8813-8825 ◽  
Author(s):  
Reinhard Schweitzer-Stenner ◽  
Enrique Ortega ◽  
Israel Pecht
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Dimer Formation ◽  
Kinetics Of

Monoclonal antibodies against rat mast cells differentiate between subtypes

1987 ◽  
Vol 20 (3-4) ◽  
pp. 216-218 ◽  
Author(s):  
H. Repke ◽  
N. Rossow ◽  
S. Savoly ◽  
J. Odarjuk ◽  
L. Karawajew ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Rat Mast Cells

Kinetics of protein phosphorylation and mediator secretion following antigen challenge of mast cells sensitized with monoclonal IgE or with reaginic serum

1997 ◽  
Vol 46 (13) ◽  
pp. 21-22
Author(s):  
C. Guérin-Marchand ◽  
T. Michon ◽  
B. Kolago ◽  
F. Marchand ◽  
A. Weyer ◽  
...  
Keyword(s):  
Mast Cells ◽  
Protein Phosphorylation ◽  
Antigen Challenge ◽  
Mediator Secretion ◽  
Kinetics Of

Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase

1990 ◽  
Vol 162 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Andrew F. Walls ◽  
David B. Jones ◽  
Julie H. Williams ◽  
Martin K. Church ◽  
Stephen T. Holgate
Keyword(s):  
Monoclonal Antibodies ◽  
Mast Cells ◽  
Fixed Tissue ◽  
Immunohistochemical Identification

scholarly journals Method for the Selective Measurement of Amino-Terminal Variants of Procalcitonin

2009 ◽  
Vol 55 (9) ◽  
pp. 1672-1679 ◽  
Author(s):  
Joachim Struck ◽  
Martina Strebelow ◽  
Sonja Tietz ◽  
Christine Alonso ◽  
Nils G Morgenthaler ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bacterial Infections ◽  
Cross Reactivity ◽  
Functional Assay ◽  
Human Endotoxemia ◽  
Amino Terminal ◽  
Endotoxemia Model ◽  
Sandwich Assays ◽  
Kinetics Of

Abstract Background: Procalcitonin (PCT) is an established marker for diagnosing and monitoring bacterial infections. Full-length PCT [116 amino acids that make up procalcitonin (PCT1–116)] can be truncated, leading to des-Ala-Pro-PCT (des-Alanin-Prolin-Procalcitonin; PCT3–116). Current immunoassays for PCT (“total PCT”) use antibodies directed against internal epitopes and are unable to distinguish amino-terminal PCT variants. Here we describe the development of monoclonal antibodies recognizing the amino-termini of PCT1–116 and PCT3–116 and their use in the selective measurement of these PCT species. Methods: With newly developed monoclonal antibodies against the amino-termini of PCT1–116 and PCT3–116, and an antibody against the katacalcin moiety of PCT, we developed and characterized immunoluminometric assays for the 2 PCT peptides. We comparatively assessed the kinetics of PCT variants in a human endotoxemia model. Results: Monoclonal antibodies against the amino-termini of PCT1–116 and PCT3–116 showed <1% cross-reactivity with other PCT-related peptides. The sandwich assays for PCT1–116 and PCT3–116 had functional assay sensitivities of 5 and 1.2 pmol/L, respectively, and exhibited recoveries within 20% of expected values. Plasma PCT1–116 was stable for 6 h at 22 °C and 24 h at 4 °C, and PCT3–116 was stable for at least 24 h at both temperatures. During experimental endotoxemia in healthy people, both PCT1–116 and PCT3–116 increased early in parallel with total PCT, but further increases in PCT1–116 were significantly slower than for PCT3–116 (P = 0.0049) and total PCT (P = 0.0024). Conclusions: The new assays selectively measure PCT1–116 and PCT3–116. Both PCT species increase early during endotoxemia but differ in their kinetics thereafter. The selective measurement of PCT species with different in vivo kinetics may be useful in improving PCT-guided therapies.

Kinetics of oxygen metabolism indices in the course of histamine secretion from rat mast cells

1990 ◽  
Vol 30 (1-2) ◽  
pp. 85-88 ◽  
Author(s):  
I. S. Gushchin ◽  
I. M. Petyaev ◽  
O. R. Tsinkalovsky
Keyword(s):  
Mast Cells ◽  
Oxygen Metabolism ◽  
Histamine Secretion ◽  
Kinetics Of ◽  
Rat Mast Cells
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