New method of separating the typhoid bacillus from the bacillus coli communis, with notes on some tests for the typhoid bacillus in pure cultures

1897 ◽  
Vol 4 (4) ◽  
pp. 429-438
Author(s):  
F. Wallis Stoddart
Keyword(s):  
New Method ◽  
Typhoid Bacillus ◽  
Pure Cultures ◽  
Bacillus Coli

Meetings of medical societies; Kazan branch of the All-Russian Society of Microbiologists and Epidemiologists. Meeting 14 / XII 36

1937 ◽  
Vol 33 (7) ◽  
pp. 942-942
Author(s):  
V. Popov
Keyword(s):  
Nutrient Medium ◽  
New Method ◽  
Russian Society ◽  
Medical Societies ◽  
Pure Cultures

Dr. P.P. Horst. On a new method for isolating pure cultures of tbc bacilli.The speaker reported on the use of a nutrient medium made by the method of Assoc. Mazura for the isolation of pure cultures of TBK.

scholarly journals THE ACTION OF X-RAYS ON EUPLOTES TAYLORI AND ASSOCIATED BACTERIA

1933 ◽  
Vol 16 (3) ◽  
pp. 397-406 ◽  
Author(s):  
Morden G. Brown ◽  
J. Murray Luck ◽  
Grace Sheets ◽  
C. V. Taylor
Keyword(s):  
Pseudomonas Fluorescens ◽  
Lethal Dose ◽  
Ciliary Activity ◽  
X Rays ◽  
Associated Bacteria ◽  
Pure Cultures ◽  
Bacillus Coli ◽  
Minimum Lethal Dose

1. The minimum lethal dose of x-rays for Euplotes taylori was determined. Under the conditions of this investigation a 220 second exposure (2110 Roentgen units per second) was required to kill the protozoon. Much less exposure was sufficient to kill the associated bacteria. This difference in resistance permits the sterilization of protozoa with comparative ease. 2. Irradiation of Euplotes for 100 to 220 seconds caused a complete but temporary cessation of ciliary activity in many of the organisms, the percentage so affected increasing with the length of irradiation. 3. Pure cultures of Pseudomonas fluorescens and Bacillus coli, K13, separately irradiated, were found to be killed much more readily than protozoa,—the former in 15 seconds exposure (2530 Roentgen units per second) and the latter in 45 seconds. 4. The death of these organisms by irradiation was not due to the action of toxic products in the medium since separately irradiated media were not found to be toxic. 5. Irradiated bacteria were found unsatisfactory for the nutrition of Euplotes, previously sterilized either by irradiation or washing.

The Ætiology of the Confusional Syndrome and the Use of T.A.B. Vaccine

1938 ◽  
Vol 84 (350-351) ◽  
pp. 563-573 ◽  
Author(s):  
D. N. Parfitt
Keyword(s):  
Typhoid Fever ◽  
Specific Protein ◽  
Foreign Protein ◽  
Typhoid Vaccine ◽  
Protein Therapy ◽  
Gram Negative ◽  
Typhoid Bacillus ◽  
Other Substances ◽  
Bacillus Coli ◽  
Gram Negative Organisms

Since the discovery that Bacillus coli vaccine (1) or albumose (2) were as effective as typhoid vaccine (3) in the treatment of typhoid fever, the use of non-specific protein therapy has been popular for almost any disease for which there is no other satisfactory form of treatment. T.A.B. vaccine, a mixture of typhoid and paratyphoid A and B organisms, has been found more regular and reliable in its results than typhoid vaccine (4); but milk, antitoxic sera, peptone and a variety of other substances have been used, and even malaria is considered a form of this treatment, segmentation of the parasites producing the foreign protein (5). It has been observed that Gram-negative organisms, such as the gonococcus and typhoid bacillus, produce more marked reactions when given intravenously than the Gram-positive organisms, such as staphylococci, streptococci and pneumococci (5).

scholarly journals THE INFLUENCE OF ENVIRONMENT UPON THE BIOLOGICAL PROCESSES OF THE VARIOUS MEMBERS OF THE COLON GROUP OF BACILLI

1897 ◽  
Vol 2 (5) ◽  
pp. 549-591 ◽  
Author(s):  
Adelaide Ward Peckham
Keyword(s):  
The Other ◽  
Biological Processes ◽  
Functional Equilibrium ◽  
Typhoid Bacillus ◽  
Artificial Environment ◽  
Serum Test ◽  
Bacillus Coli ◽  
Biological Relationship ◽  
Increased Activity ◽  
Methods Of Treatment

Assuming the typical colon bacillus and the typical typhoid bacillus to represent the types of this group that present the greatest divergences in biological peculiarities, we conclude, as others also have done, that there is a series of closely related forms that may be regarded as intermediate or transitional and which serve to establish a biological relationship, either near or remote, between these two typical members. From our own studies we are inclined to regard the typical colon bacillus as the type of this group, for the reason that its functional equilibrium, as observed in the intestine, is so permanent a quality that it may readily be perpetuated under what is ordinarily regarded as favorable artificial circumstances, and that with the continuance of such conditions there is no conspicuous tendency on the part of this organism to deviate from what we regard as its norm; whereas, on the other hand, with all the other members of this group with which we have worked, there is not only a lack of uniformity in the adjustment of the functions, but such as exists is readily disturbed under artificial environment; though it must be borne in mind that even with the typical colon bacillus we have also shown functional modifications to be possible under particular conditions. When the members of the colon group are cultivated under circumstances favorable to the development of both the function of fermentation and that of proteolysis, fermentation invariably takes precedence and no evidence of proteolysis is manifested until after fermentation has ceased. The cultivation of all the members of the colon group under circumstances that favor the development of one function, viz. that of proteolysis, at the expense of another, viz. that of fermentation, results first in an apparent increase of vigor; but this is of temporary duration and is quickly followed by the decline and death of the cell. The result of this increased activity of the proteolytic function is the formation of much larger amounts of indol by typical colon cultures than has ever been obtained by us by any other method. By the method of experimentation through which we were enabled to accentuate the proteolytic activity of the typical colon bacillus, as caused by an increase of indol formation, we have also induced the function of indol formation not only in atypical colon bacilli that had been devoid of it, but in every specimen of typical typhoid bacilli to which we had access as well. We feel justified in regarding one of the differential tests between the typhoid and colon bacillus, namely that of indol formation on the part of the latter and the absence of this function from the former, as of questionable value, for the reason, as shown above, that by particular methods of cultivation indol production has been shown to accompany the development of a number of specimens that we have every reason to regard as genuine typhoid bacilli. As a result of our own experiments, together with the observations of others, there can be no doubt that the bacillus coli communis at times possesses pathogenic properties, and that by artificial methods of treatment it may often be brought from a condition of benignity to one of virulence. The spleen of a typhoid patient has always been regarded as the only trustworthy source from which to obtain the typical typhoid bacillus. While we believe this to be true, still our investigations show that other members of the colon group may also be present in this viscus; in fact, from such spleens we have isolated practically all of the varieties of this group with which we are acquainted. From our experience, the value of the serum test for the differentiation of typhoid and colon bacilli would seem to be questionable. We are inclined, however, to attribute the irregularities recorded above as due more to the method of application than to defects of the principles involved; for, as stated, by the use of dried blood, as in our experiments, it is not possible to make the test with constant and accurate, or even approximately accurate, dilutions of the serum. Our irregularities may be in part due to this defect. We therefore lay less stress upon this than upon the other features of our work.

A new method for isolating pure cultures

1926 ◽  
Vol 22 (5-6) ◽  
pp. 733
Author(s):  
V. Popov
Keyword(s):  
Bacterial Cell ◽  
Nutrient Medium ◽  
New Method ◽  
Experimental Animals ◽  
Liquid Nutrient Medium ◽  
Glass Slides ◽  
Pure Cultures

Peterfi and Wamoscher (Zeit. Fr Hygiene, 1926, Bd. 106, H. 1), in order to obtain pure cultures, carried out the finest manipulations with bacteria under control in Dunkelfeld: using a special chamber from glass slides and a micropipette, any bacterial cell and, transferring it to another similar chamber with a liquid nutrient medium, the process of reproduction of the microbe (Einzellkulturen) was observed under a microscope. Using this method, the authors also succeeded in infecting experimental animals with only one pneumococcal cell in a number of cases.

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