Meetings of medical societies; Kazan branch of the All-Russian Society of Microbiologists and Epidemiologists. Meeting 14 / XII 36

1937 ◽  
Vol 33 (7) ◽  
pp. 942-942
Author(s):  
V. Popov
Keyword(s):  
Nutrient Medium ◽  
New Method ◽  
Russian Society ◽  
Medical Societies ◽  
Pure Cultures

Dr. P.P. Horst. On a new method for isolating pure cultures of tbc bacilli.The speaker reported on the use of a nutrient medium made by the method of Assoc. Mazura for the isolation of pure cultures of TBK.

A new method for isolating pure cultures

1926 ◽  
Vol 22 (5-6) ◽  
pp. 733
Author(s):  
V. Popov
Keyword(s):  
Bacterial Cell ◽  
Nutrient Medium ◽  
New Method ◽  
Experimental Animals ◽  
Liquid Nutrient Medium ◽  
Glass Slides ◽  
Pure Cultures

Peterfi and Wamoscher (Zeit. Fr Hygiene, 1926, Bd. 106, H. 1), in order to obtain pure cultures, carried out the finest manipulations with bacteria under control in Dunkelfeld: using a special chamber from glass slides and a micropipette, any bacterial cell and, transferring it to another similar chamber with a liquid nutrient medium, the process of reproduction of the microbe (Einzellkulturen) was observed under a microscope. Using this method, the authors also succeeded in infecting experimental animals with only one pneumococcal cell in a number of cases.

scholarly journals A METHOD FOR THE PHYSIOLOGICAL STUDY OF TISSUES IN VITRO

1923 ◽  
Vol 38 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Alexis Carrel
Keyword(s):  
Nutrient Medium ◽  
Bacterial Contamination ◽  
Culture Medium ◽  
Residual Activity ◽  
Physical Factors ◽  
Dynamic Changes ◽  
Fluid Medium ◽  
Continuous Growth ◽  
Pure Cultures

1. A method has been developed which allows the continuous growth of pure strains of fibroblasts, epithelium, and leucocytes in a medium which undergoes but slight spontaneous deterioration. 2. The principle of the method is to leave the tissues undisturbed while the medium is changed. This was realized by special containers allowing the change of the medium without bacterial contamination and by the simultaneous use of a solid and a fluid medium. 3. The curve of growth of pure cultures of fibroblasts and epithelial cells in a nutrient medium is a parabola; in a non-nutrient medium, it is S-shaped and expresses the residual activity of the tissues. Leucocytes invade the culture medium progressively, as do bacteria, but never aggregate in a tissue. 4. The method is used for the study of the morphological and dynamic changes occurring in tissues under the influence of chemical and physical factors.

Letter to the editor

1908 ◽  
Vol 8 (11-12) ◽  
pp. 624
Keyword(s):  
Russian Society ◽  
Letter To The Editor ◽  
Medical Societies ◽  
Draft Statute

The Board of the Society of Russian Doctors in memory of N.I. Pirogov asks you not to refuse to print in your edition of the "Basic provisions of the draft statute" of the All-Russian Society of Physicians in memory of N.I. Pirogov and the following appeal to medical societies and individual doctors of Pirogov: The congress of doctors elected a commission of 12 persons, which, together with the Board of the Pirogov Society, should revise the charter of the Pirogov Society and develop a draft charter of the Pirogov All-Russian Union of Physicians.

Meetings of medical societies; Kazan branch of the All-Russian Society of Microbiologists and Epidemiologists. Meeting 26 / XI 1936

1937 ◽  
Vol 33 (7) ◽  
pp. 942-942
Author(s):  
V. Popov
Keyword(s):  
Russian Society ◽  
Medical Societies

Opening remarks dedicated to the memory of prof. N. P. Rufimsky, says the chairman of the society prof. R. R. Geltzer.

scholarly journals Decapsulation of Dextran by Destruction of Polyelectrolyte Microcapsule Nanoscale Shell by Bacillus subtilis Bacteria

Nanomaterials ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 12
Author(s):  
Egor V. Musin ◽  
Aleksandr L. Kim ◽  
Alexey V. Dubrovskii ◽  
Ekaterina B. Kudryashova ◽  
Sergey A. Tikhonenko
Keyword(s):  
Bacillus Subtilis ◽  
Nutrient Medium ◽  
Bacterial Spore ◽  
New Method ◽  
Bacterial Spores ◽  
Polyelectrolyte Microcapsules ◽  
Membrane Shell ◽  
Polyelectrolyte Microcapsule ◽  
Polyallylamine Hydrochloride

One of the prerequisites of successful address delivery is controlling the release of encapsulated drugs. The new method of bacterial spore encapsulation in polyelectrolyte microcapsules allows for degrading the nanoscale membrane shell of microcapsules. The possibility of encapsulating spore forms of Bacillus subtilis in polystyrenesulfonate sodium/ polyallylamine hydrochloride (PSS/PAH) polyelectrolyte microcapsules was demonstrated. The activation and growth on a nutrient medium of encapsulated bacterial spores led to 60% degradation of the microcapsules nanoscale membrane shell. As a result, 18.5% of Fluorescein isothiocyanatedextran was encapsulated into polyelectrolyte microcapsules, and 28.6% of the encapsulated concentration of FITC-dextran was released into the solution.

STRAIN SELECTION BRADYRHIZOBIUM JAPONICUM AND SINORHIZOBIUM FREDII TO RESISTANT TO AMMONIUM MOLYBDATE IN GROWING MEDIUM

1970 ◽  
pp. 53-56
Author(s):  
М. V. Yakimenko ◽  
S. А. Begun ◽  
А. I. Sorokina
Keyword(s):  
Nutrient Medium ◽  
Bradyrhizobium Japonicum ◽  
Ammonium Molybdate ◽  
Good Growth ◽  
Sinorhizobium Fredii ◽  
Poor Growth ◽  
Moderate Growth ◽  
Growth Intensity ◽  
Pure Cultures ◽  
Bacterial Mass

The article presents the results of the study of the resistance of Bradyrhizobium japonicum (Jordan, 1982) and Sinorhizobium fredii (Scholla, Elkan, 1984) strains, selection of the FSBSI ARSRI of Soybean, to ammonium molybdate in a nutrient medium. In the variant without introducing a molybdenum salt, from 47 strains of rhizobia of the B. japonicum species, 37 strains showed good growth, 3 strains-abundant and 7 strains-moderate growth of the bacterial mass stroke. When 1 g/l of ammonium molybdate was introduced into the nutrient medium, the growth intensity of the bacterial mass stroke of all tested B. japonicum decreased slightly; the number of moderately growing strains increased to 11, and one poorly growing strain appeared. When 10 g/l of ammonium molybdate was introduced into the nutrient medium, the strains of B. japonicum, showing abundant and good growth of bacterial mass, were not detected. 31 strains of rhizobia of this species have stopped their growth. In the variants with a nutrient medium MRS without a molybdenum salt and with 1 g/l molybdenum salt from 44 strains of rhizobia of the species S. fredii 93-95 % of pure cultures showed abundant and good bacterial mass stroke growth. When 10 g/l of ammonium molybdate was introduced into the nutrient medium, no rhizobia strains of the S. fredii species were found with abundant and good growth of the bacterial mass, 12 rhizobia strains of this species showed poor growth, and 4 strains stopped growing at this concentration of molybdenum salt in the nutrient environment. It was established that ammonium molybdate at a concentration of 1 g/l in a nutrient medium, practically does not affect the growth intensity of the bacterial mass stroke of strains B. japonicum and S. fredii.

Determination of Ammonia Inhibition in Fabric Impregnated with Antimicrobials

1975 ◽  
Vol 58 (5) ◽  
pp. 998-1000
Author(s):  
Frederic H Wilson ◽  
William Ball
Keyword(s):  
Proteus Mirabilis ◽  
Nutrient Medium ◽  
Color Change ◽  
New Method ◽  
Ammonia Inhibition ◽  
Petri Dishes ◽  
Overnight Culture ◽  
Circular Disks ◽  
Production Of Ammonia

Abstract A new method is described for determining the presence or absence of ammonia produced by urealytic microorganisms, using fabric which has been treated with an antimicrobial. Circular disks of fabric treated with various concentrations of an antimicrobial are placed in the bottoms of snap-on lid petri dishes. Nutrient medium containing urea, an indicator, and a dilution of an overnight culture of Proteus mirabilis is applied to the fabric disks. The lids are snapped on and the dishes are incubated at 37°C. By examination of the fabric disks at regular intervals of time for a color change from white to red, one may determine whether the concentration of antimicrobial in the fabric is sufficient to inhibit the production of ammonia by a urealytic microorganism.

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