scholarly journals Development of a submerged culture method for high production of acid- stable α-amylase and glucoamylase usingAspergillus kawachiiwithout glucose concentration control

2012 ◽  
Vol 118 (4) ◽  
pp. 346-351 ◽  
Author(s):  
S. Masuda ◽  
H. Shoji
Keyword(s):  
Glucose Concentration ◽  
Submerged Culture ◽  
Culture Method ◽  
High Production ◽  
Acid Stable

Characteristics of acid-stable α-amylase production by submerged culture of Aspergillus kawachii

1993 ◽  
Vol 76 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Shigetoshi Sudo ◽  
Takeaki Ishikawa ◽  
Yuko Takayasu-Sakamoto ◽  
Kazuo Sato ◽  
Toshiteru Oba
Keyword(s):  
Submerged Culture ◽  
Amylase Production ◽  
Aspergillus Kawachii ◽  
Acid Stable

scholarly journals Potential of marula (Sclerocarya birrea subsp. caffra) waste for the production of vinegar through surface and submerged fermentation

2018 ◽  
Vol 114 (11/12) ◽  
Author(s):  
Tumisi J. Molelekoa ◽  
Thierry Regnier ◽  
Laura S. da Silva ◽  
Wilma A. Augustyn
Keyword(s):  
South Africa ◽  
Acetic Acid ◽  
Bioactive Compounds ◽  
Submerged Fermentation ◽  
Submerged Culture ◽  
Starter Culture ◽  
Culture Method ◽  
Limpopo Province ◽  
Surface Culture ◽  
Culture Techniques

Although there is an abundance of indigenous fruits in South Africa, knowledge of their potential uses is mainly restricted to within communities. In this study, marula fruit-processing waste by-products (fruit pulp residue and skin) were used as substrates in surface culture and submerged fermentation methods to produce vinegar (acetic acid) using spontaneous and starter culture techniques. The study revealed the possibility of producing vinegar through both methods of fermentation, with yields of acetic acid ranging between 41 000 mg/L and 57 000 mg/L (surface culture method) and between 41 000 and 54 000 mg/L (submerged culture method). Furthermore, the physicochemical property analyses revealed marula vinegar to be a potential source of bioactive compounds (total phenolics 0.289–0.356 mg/L GAE and total flavonoids 0.146–0.153 mg/L CAE) which displayed a potent antiradical activity against DPPH•: 78.85% for surface culture and 73.03% submerged culture, respectively. The sensory panel recommended application of the vinegar in products such as salad dressing and mayonnaise. Finally, we have demonstrated that the surface culture method using the inoculation technique is more suitable for the production of high-quality vinegar, with possible consideration for commercialisation. Significance: Marula fruit has high economic importance for South Africa, particularly for the Limpopo Province. Marula waste can be a source of bioactive compounds, yet comparatively little is reported on the potential use of the waste to produce vinegar. Self-development of communities through viable and easy to produce commodities from marula fruit needs to be implemented and prioritised in the Limpopo Province.

scholarly journals Determination the optimum condition for production of superoxide dismutase (SOD) from a local isolate of staphylococcus aureus

2009 ◽  
Vol 3 (2) ◽  
pp. 63-75
Author(s):  
Hala Abd- Alkariem Rasheed ◽  
Ghazi Munim Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Superoxide Dismutase ◽  
Submerged Culture ◽  
Enzyme Production ◽  
Seminal Fluid ◽  
Nitrogen Sources ◽  
Culture Method ◽  
Optimal Time ◽  
Vaginal Swabs ◽  
Initial Ph

Thirty three isolates classified as Staphylococcus aureus were isolated from 183 different samples that included wounds, burns, boils, abscesses, ear, nose, vaginal swabs, blood, urine samples, sputum, seminal fluid and cerebrospinal fluid. The ability of these isolates to produce superoxide dismutase (SOD) was tested by using submerged culture. It has been found that the isolate S.aureus HM86 has the highest productivity of the enzyme. The optimal condition for SOD enzyme production from the isolate S.aureus HM86 by the submerged culture method were determined using (0.5 %) Mannose as carbon source and peptone (0.5%) and pancreatic digest of casein (2%) as nitrogen sources with initial pH of 7 after 12 hours of incubation at 37oC, in shaker incubator at 150 rpm and with aeration ratio (1: 6.6). It has been found that the best method for enzyme extraction is by using ultrasonication for 15 min, the optimal time for addition of paraquat at concentration of 0.5 mM is after 6 and 8 hours of growth.

scholarly journals Cage culture of Oreochromis Mossambicus (Tilapia) in back water of river Godavari, Nanded, Maharashtra India

2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Jayvardhan V Balkhande
Keyword(s):  
Culture Method ◽  
Oreochromis Mossambicus ◽  
Cage Culture ◽  
High Production ◽  
River Godavari ◽  
Day By Day

Cage culture practice is an important technology for culture of finfish and non fin fish organisms. It is gaining importance day by day. This was a first attempt to check the suitability of cage culture in back water of Godavari. This water remains present up to March and April; hence this experiment was carried out from October 2012 to April 2013 (180 days) at Dhangar Takli on the bank of river Godavari. Results showed that Oreochromis mossambicus fish was useful fish for cage culture practice in this area. Although, the Monosex culture of Oreochromis mossambicus may be more applicable because this fish is prolific breeder. If we alter the breeding of fish then this will may help to increase the growth of the fish. Oreochromis mossambicus commonly called as freshwater pomfret. People of this area consume this fish is in high quantity; hence for high production this cage culture method may be helpful.

scholarly journals Production of Diphtheria Toxin by the Submerged Culture Method

Nature ◽  
1946 ◽  
Vol 157 (3983) ◽  
pp. 264-264
Author(s):  
F. V. LINGGOOD
Keyword(s):  
Diphtheria Toxin ◽  
Submerged Culture ◽  
Culture Method

Simultaneous production of glucoamylase and acid-stable α-amylase using novel submerged culture of Aspergillus kawachii NBRC4308

2007 ◽  
Vol 103 (2) ◽  
pp. 203-205 ◽  
Author(s):  
Hiroshi Shoji ◽  
Toshikazu Sugimoto ◽  
Kenji Hosoi ◽  
Kazunori Shibata ◽  
Masayuki Tanabe ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Simultaneous Production ◽  
Aspergillus Kawachii ◽  
Acid Stable

6-Aminopenicillanic acid II. Formation of 6-aminopenicillanic acid by Emericellopsis minima (Stolk) and related fungi

1961 ◽  
Vol 154 (957) ◽  
pp. 490-497 ◽  
Keyword(s):  
Submerged Culture ◽  
Penicillin Amidase ◽  
Amidase Activity ◽  
Acid Stable

In submerged culture, Emericellopsis minima (Stolk) I. M. I. 69015 produced a substance resembling 6-aminopenicillanic acid in its behaviour on paper chromatograms and in its properties after reaction with phenylacetyl chloride. The substance was destroyed by peni­cillinase but was acid-stable. The culture also produced cephalosporin N and two unidentified antibiotics which have been named emericellopsin A and B . When other fungi of the genera Emericellopsis and Gephalosporium were examined in a similar manner, C , salmosynnematum and Cephalosporium I. M. I. 49137 were also found to produce small amounts of a substance resembling 6-aminopenicillanic acid. Examination of Emericellopsis minima (Stolk) also showed the presence of penicillin amidase activity in this culture.

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

“Blastosphere”: A new culture method for human fetal hepatic progenitor cells

2001 ◽  
Vol 120 (5) ◽  
pp. A542-A543
Author(s):  
T HIROSE ◽  
K YASUCHIKA ◽  
T FUJIKAWA ◽  
H FUJII ◽  
S OE ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Culture Method ◽  
Hepatic Progenitor Cells
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