Video-rate hyperspectral two-photon fluorescence microscopy for in vivo imaging

2018 ◽  
Author(s):  
Fengyuan Deng ◽  
Changqin Ding ◽  
Jerald C. Martin ◽  
Nicole M. Scarborough ◽  
Zhengtian Song ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Two-Photon Microscopy Allows Imaging and Characterization of Cochlear Microvasculature In Vivo

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Friedrich Ihler ◽  
Mattis Bertlich ◽  
Bernhard Weiss ◽  
Steffen Dietzel ◽  
Martin Canis
Keyword(s):  
Fluorescence Microscopy ◽  
Inner Ear ◽  
Ex Vivo ◽  
Small Scale ◽  
Published Data ◽  
Cochlear Blood Flow ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Impairment of cochlear blood flow has been discussed as factor in the pathophysiology of various inner ear disorders. However, the microscopic study of cochlear microcirculation is limited due to small scale and anatomical constraints. Here, two-photon fluorescence microscopy is applied to visualize cochlear microvessels. Guinea pigs were injected with Fluorescein isothiocyanate- or Texas red-dextrane as plasma marker. Intravital microscopy was performed in four animals and explanted cochleae from four animals were studied. The vascular architecture of the cochlea was visualized up to a depth of90.0±22.7 μm. Imaging yielded a mean contrast-to-noise ratio (CNR) of3.3±1.7. Mean diameter in vivo was16.5±6.0 μm for arterioles and8.0±2.4 μm for capillaries. In explanted cochleae, the diameter of radiating arterioles and capillaries was measured with12.2±1.6 μm and6.6±1.0 μm, respectively. The difference between capillaries and arterioles was statistically significant in both experimental setups (P<0.001andP=0.022, two-way ANOVA). Measured vessel diameters in vivo and ex vivo were in agreement with published data. We conclude that two-photon fluorescence microscopy allows the investigation of cochlear microvessels and is potentially a valuable tool for inner ear research.

Fast handheld two-photon fluorescence microendoscope with a 475 μm × 475 μm field of view for in vivo imaging

2008 ◽  
Vol 33 (12) ◽  
pp. 1333 ◽  
Author(s):  
Hongchun Bao ◽  
John Allen ◽  
Robert Pattie ◽  
Rod Vance ◽  
Min Gu
Keyword(s):  
In Vivo Imaging ◽  
Field Of View ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

2020 ◽  
Vol 17 (3) ◽  
pp. 287-290 ◽  
Author(s):  
Jianglai Wu ◽  
Yajie Liang ◽  
Shuo Chen ◽  
Ching-Lung Hsu ◽  
Mariya Chavarha ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neural Activity ◽  
Microscopy Imaging ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Synthesis and evaluation of novel two-photon fluorescence probes for in vivo imaging of amylin aggregates in the pancreas

2019 ◽  
Vol 170 ◽  
pp. 107615 ◽  
Author(s):  
Hiroyuki Watanabe ◽  
Yusuke Miki ◽  
Yoichi Shimizu ◽  
Hideo Saji ◽  
Masahiro Ono
Keyword(s):  
In Vivo Imaging ◽  
Fluorescence Probes ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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