Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

ChemPhysChem ◽  
2010 ◽  
Vol 11 (5) ◽  
pp. 1011-1017 ◽  
Author(s):  
Lisa Simonsson ◽  
Peter Jönsson ◽  
Gudrun Stengel ◽  
Fredrik Höök
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Site Specific ◽  
Controlled Fusion

scholarly journals Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

2010 ◽  
Vol 98 (3) ◽  
pp. 673a
Author(s):  
Lisa V. Simonsson ◽  
Peter Jönsson ◽  
Gudrun Stengel ◽  
Fredrik Höök
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Site Specific ◽  
Controlled Fusion

scholarly journals Inside Cover: Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers (ChemPhysChem 5/2010)

ChemPhysChem ◽  
2010 ◽  
Vol 11 (5) ◽  
pp. 926-926 ◽  
Author(s):  
Lisa Simonsson ◽  
Peter Jönsson ◽  
Gudrun Stengel ◽  
Fredrik Höök
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Site Specific ◽  
Controlled Fusion

scholarly journals The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

Soft Matter ◽  
2018 ◽  
Vol 14 (28) ◽  
pp. 5764-5774 ◽  
Author(s):  
F. Mousseau ◽  
J.-F. Berret
Keyword(s):  
Surface Charge ◽  
Lipid Bilayers ◽  
Pulmonary Surfactant ◽  
Lipid Vesicles ◽  
Electrostatic Charge ◽  
Supported Lipid Bilayers ◽  
Pulmonary Surfactants ◽  
Inhaled Nanoparticles

Inhaled nanoparticles reaching the respiratory zone in the lungs enter first in contact with the pulmonary surfactant. It is shown here that nanoparticles and lipid vesicles formulated from different surfactant mimetics interact predominantlyviaelectrostatic charge mediated attraction and do not form supported lipid bilayers spontaneously.

scholarly journals Correlative microscopy reveals the nanoscale morphology of E. coli-derived supported lipid bilayers

2021 ◽  
Author(s):  
Karan Bali ◽  
Zeinab Mohamed ◽  
Anna-Maria Pappa ◽  
Susan Daniel ◽  
Clemens F. Kaminski ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Lipid Vesicles ◽  
Outer Membrane Vesicles ◽  
Atomic Scale ◽  
Membrane Properties ◽  
Supported Lipid Bilayers ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Natural And Synthetic

Supported lipid bilayers (SLBs) made from reconstituted lipid vesicles are an important tool in molecular biology. A breakthrough in the field has come with the use of vesicles derived from cell membranes to form SLBs. These new supported bilayers, consisting both of natural and synthetic components, provide a physiologically relevant system on which to study protein-protein interactions as well as protein-ligand interactions and other lipid membrane properties. These complex bilayer systems hold promise but have not yet been fully characterised in terms of their composition, ratio of natural to synthetic component and membrane protein content. Here, we describe a method of correlative atomic force (AFM) with structured illumination microscopy (SIM) for the accurate mapping of complex lipid bilayers that consist of a synthetic fraction and a fraction of lipids derived from Escherichia coli outer membrane vesicles (OMVs). We exploit the enhanced resolution and molecular specificity that SIM can offer to identify areas of interest in these bilayers and the atomic scale resolution that the AFM provides to create detailed topography maps of the bilayers. We are thus able to understand the way in which the two different lipid fractions (natural and synthetic) mix within the bilayers, quantify the amount of bacterial membrane incorporated in the bilayer and directly visualise the interaction of these bilayers with bacteria-specific, membrane-binding proteins. Our work sets the foundation for accurately understanding the composition and properties of OMV-derived SLBs and establishes correlative AFM/ SIM as a method for characterising complex systems at the nanoscale.

scholarly journals Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregation from Supported Lipid Bilayer Formation

2018 ◽  
Vol 2 (4) ◽  
pp. 50 ◽  
Author(s):  
Fanny Mousseau ◽  
Evdokia Oikonomou ◽  
Victor Baldim ◽  
Stéphane Mornet ◽  
Jean-François Berret
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Living Cells ◽  
Supported Lipid Bilayers ◽  
Supported Lipid Bilayer ◽  
Mixed Aggregates ◽  
Method Of Continuous Variation ◽  
The Impact ◽  
Analytical Expressions ◽  
Lipid Interaction

The impact of nanomaterials on lung fluids, or on the plasma membrane of living cells, has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Currently, there is a need to have simple protocols that can readily evaluate the structures made from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between experiments and modeling is that electrostatics play a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

scholarly journals Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregationfrom Supported Lipid Bilayer Formation

2018 ◽  
Author(s):  
Fanny Mousseau ◽  
Evdokia Oikonomou ◽  
Victor Baldim ◽  
Stéphane Mornet ◽  
Jean-François Berret
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Living Cells ◽  
Supported Lipid Bilayers ◽  
Supported Lipid Bilayer ◽  
Mixed Aggregates ◽  
Method Of Continuous Variation ◽  
The Impact ◽  
Analytical Expressions ◽  
Lipid Interaction

The impact of nanomaterials on lung fluids or on the plasma membrane of living cells has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Today, there is a need to have simple protocols that can readily assess the nature of structures obtained from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between modeling and experimental measurements is that electrostatics plays a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

Engineered Site-specific Vesicular Systems for Colonic Delivery: Trends and Implications

2020 ◽  
Vol 26 (42) ◽  
pp. 5441-5455
Author(s):  
Honey Goel ◽  
Karan Razdan ◽  
Richu Singla ◽  
Sushama Talegaonkar ◽  
Rajneet Kaur Khurana ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Targeted Delivery ◽  
Oral Delivery ◽  
Low Cost ◽  
Lipid Vesicles ◽  
Oral Route ◽  
Peptidase Activity ◽  
Colonic Delivery ◽  
Site Specific ◽  
Drug Degradation

Steering drug-loaded, site-specific, coated lipid vesicles to the target receptor sites have the potential of plummeting adverse effects and improving the pharmacological response in diverse pathologies of the large bowel, especially the colon. Colonic delivery via oral route has its own challenges, often governed by several glitches such as drug degradation or absorption in the upper GIT, instability of proteins/peptides due to high molecular weight, and peptidase activity in the stomach. Consequently, colon-specific coated liposomal systems (CSLS) offer a potential alternate for not only site-specificity, but protection from proteolytic activity, and prolonged residence time for greater systemic bioavailability. On the other hand, liposomal delivery via the oral route is also cumbersome owing to several barriers such as instability in GIT, difficulty in crossing membranes, and issues related to production at the pilot scale. New advancements in the field of CSLS have successfully improved the stability and permeability of liposomes for oral delivery via modulating the compositions of lipid bilayers, adding polymers or ligands. Despite this ostensible propitiousness, no commercial oral CSLS has advanced from bench to bedside for targeted delivery to the colon as yet. Nevertheless, CSLS has quite fascinated the manufacturers owing to its potential industrial viability, simplistic and low-cost design. Hence, this review aims to decipher the convolutions involved in the engineering process of industrially viable CSLS for colonic delivery.

scholarly journals Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques

2008 ◽  
Vol 3 (2) ◽  
pp. FA108-FA116 ◽  
Author(s):  
Fredrik Höök ◽  
Gudrun Stengel ◽  
Andreas B. Dahlin ◽  
Anders Gunnarsson ◽  
Magnus P. Jonsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Vesicles ◽  
Vesicle Fusion ◽  
Supported Lipid Bilayers ◽  
Microscopy Techniques

Spatial-Resolution Limits in Mass Spectrometry Imaging of Supported Lipid Bilayers and Individual Lipid Vesicles

2010 ◽  
Vol 82 (6) ◽  
pp. 2426-2433 ◽  
Author(s):  
Anders Gunnarsson ◽  
Felix Kollmer ◽  
Sascha Sohn ◽  
Fredrik Höök ◽  
Peter Sjövall
Keyword(s):  
Mass Spectrometry ◽  
Lipid Bilayers ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Lipid Vesicles ◽  
Supported Lipid Bilayers ◽  
Resolution Limits

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

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